scholarly journals The CbrA-CbrB two-component regulatory system controls the utilization of multiple carbon and nitrogen sources in Pseudomonas aeruginosa

2001 ◽  
Vol 40 (4) ◽  
pp. 917-931 ◽  
Author(s):  
Takayuki Nishijyo ◽  
Dieter Haas ◽  
Yoshifumi Itoh
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Regulatory System ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Two Component

scholarly journals Glycine Betaine Transmethylase Mutant of Pseudomonas aeruginosa

2002 ◽  
Vol 184 (15) ◽  
pp. 4301-4303 ◽  
Author(s):  
Ana L. Serra ◽  
Javier F. Mariscotti ◽  
José L. Barra ◽  
Gloria I. Lucchesi ◽  
Carlos E. Domenech ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Sequence Analysis ◽  
Glycine Betaine ◽  
Nitrogen Sources ◽  
Open Reading Frame ◽  
Carbon And Nitrogen Sources ◽  
Reading Frame ◽  
Carbon And Nitrogen ◽  
Molecular Approaches ◽  
Knockout Gene

ABSTRACT The gene for glycine betaine transmethylase (gbt) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout gene corresponded to an open reading frame (ORF) named PA3082 in the genome of P. aeruginosa PAO1. The translated product of this ORF displayed similarity to transferases of different microorganisms. Mutation in gbt blocked the utilization of choline and glycine betaine as carbon and nitrogen sources.

scholarly journals Effects of carbon and nitrogen sources on the proteome of Pseudomonas aeruginosa PA1 during rhamnolipid production

2010 ◽  
Vol 45 (9) ◽  
pp. 1504-1510 ◽  
Author(s):  
Rodrigo Siqueira Reis ◽  
Surza Lucia Gonçalves da Rocha ◽  
Donat Alexander Chapeaurouge ◽  
Gilberto Barbosa Domont ◽  
Lidia Maria Melo Santa Anna ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Rhamnolipid Production

scholarly journals Metabolism of Chicken Feathers and Concomitant Electricity Generation by Pseudomonas aeruginosa by Employing Microbial Fuel Cell (MFC)

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Venkatesh Chaturvedi ◽  
Pradeep Verma
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Nitrogen Sources ◽  
Electricity Production ◽  
Malt Extract ◽  
Feather Degradation ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen ◽  
Chicken Feathers

Keratinolytic potential of Pseudomonas aeruginosa strain SDS3 has been evaluated for the metabolism of chicken feathers. Results indicated that strain SDS3 showed complete metabolism of 0.1 and 0.5% (w/v) chicken feathers in minimal medium. Feathers were metabolized up to 80% at 1% (w/v) concentration. Maximum soluble protein (480.8±17.1 μg/mL) and keratinase (15.4±0.25 U/mL) were observed in the presence of 1% chicken feathers after five days of incubation. The effect of carbon and nitrogen sources showed that feather degradation was stimulated by complex carbon/nitrogen sources such as starch, malt extract, tryptone, and beef extract and was inhibited by simple carbon and nitrogen sources. Electricity production by employing chicken feathers as a substrate in microbial fuel cell (MFC) was evaluated. It was observed that maximum voltage corresponding to 141 mV was observed after 14 days of incubation. Maximum power density of 1206.78 mW/m2 and maximum current density of 8.6 mA/m2 were observed. The results clearly indicate that chicken feathers can be successfully employed as a cheap substrate for electricity production in MFC. This is the first report showing employment of chicken feathers as substrate in MFC.

Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of Pseudomonas aeruginosa

2002 ◽  
Vol 98-100 (1-9) ◽  
pp. 1025-1036 ◽  
Author(s):  
Alexandre S. Santos ◽  
Ana Paula W. Sampaio ◽  
Gina S. Vasquez ◽  
Lídia M. Santa Anna ◽  
Nei Pereira Jr. ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

Evaluation of Different Carbon and Nitrogen Sources in Production of Rhamnolipids by a Strain of Pseudomonas aeruginosa

2002 ◽  
pp. 1025-1035 ◽  
Author(s):  
Alexandre S. Santos ◽  
Ana Paula W. Sampaio ◽  
Gina S. Vasquez ◽  
Lídia M. Santa Anna ◽  
Nei Pereira ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Nitrogen Sources ◽  
Carbon And Nitrogen Sources ◽  
Carbon And Nitrogen

scholarly journals High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

2018 ◽  
Vol 69 ◽  
pp. 1-11 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Roselei Claudete Fontana ◽  
Simone Mendonça ◽  
Félix Gonçalves de Siqueira ◽  
Aldo José Pinheiro Dillon ◽  
...  
Keyword(s):  
Nitrogen Sources ◽  
White Rot ◽  
Stirred Tank ◽  
Carbon And Nitrogen Sources ◽  
Stirred Tank Bioreactor ◽  
Carbon And Nitrogen ◽  
High Level ◽  
Level Production

AgmR controls transcription of a regulon with several operons essential for ethanol oxidation in Pseudomonas aeruginosa ATCC 17933

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1851-1857 ◽  
Author(s):  
Nicole Gliese ◽  
Viola Khodaverdi ◽  
Max Schobert ◽  
Helmut Görisch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Ethanol Oxidation ◽  
Response Regulator ◽  
Regulatory System ◽  
Pyrroloquinoline Quinone ◽  
Kanamycin Resistance ◽  
Kanamycin Resistance Cassette ◽  
Two Component ◽  
Ethanol Dehydrogenase ◽  
Oxidation System

The response regulator AgmR was identified to be involved in the regulation of the quinoprotein ethanol oxidation system of Pseudomonas aeruginosa ATCC 17933. Interruption of the agmR gene by insertion of a kanamycin-resistance cassette resulted in mutant NG3, unable to grow on ethanol. After complementation with the intact agmR gene, growth on ethanol was restored. Transcriptional lacZ fusions were used to identify four operons which are regulated by the AgmR protein: the exaA operon encodes the pyrroloquinoline quinone (PQQ)-dependent ethanol dehydrogenase, the exaBC operon encodes a soluble cytochrome c 550 and an aldehyde dehydrogenase, the pqqABCDE operon carries the PQQ biosynthetic genes, and operon exaDE encodes a two-component regulatory system which controls transcription of the exaA operon. Transcription of exaA was restored by transformation of NG3 with a pUCP20T derivative carrying the exaDE genes under lac-promoter control. These data indicate that the AgmR response regulator and the exaDE two-component regulatory system are organized in a hierarchical manner. Gene PA1977, which appears to form an operon with the agmR gene, was found to be non-essential for growth on ethanol.

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