scholarly journals Transcript processing internal to a mitochondrial open reading frame is correlated with fertility restoration in male-sterile sorghum

1996 ◽  
Vol 10 (1) ◽  
pp. 123-133 ◽  
Author(s):  
Hoang V. Tang ◽  
Daryl R. Pring ◽  
Lynn C. Shaw ◽  
Reggie A. Salazar ◽  
Figuhr R. Muza ◽  
...  
Keyword(s):  
Fertility Restoration ◽  
Open Reading Frame ◽  
Male Sterile ◽  
Reading Frame ◽  
Transcript Processing

scholarly journals Cosegregation of Single Genes Associated with Fertility Restoration and Transcript Processing of Sorghum Mitochondrial orf107 and urf209

Genetics ◽  
1998 ◽  
Vol 150 (1) ◽  
pp. 383-391 ◽  
Author(s):  
Hoang V Tang ◽  
Ruying Chang ◽  
Daryl R Pring
Keyword(s):  
Fertility Restoration ◽  
Mitochondrial Gene ◽  
Pollen Viability ◽  
Single Gene ◽  
Dominant Gene ◽  
Nuclear Gene ◽  
Male Sterile ◽  
Reading Frame ◽  
Transcript Processing ◽  
Backcross Populations

Abstract Defective nuclear-cytoplasmic interactions leading to aberrant microgametogenesis in sorghum carrying the IS1112C male-sterile cytoplasm occur very late in pollen maturation. Amelioration of this condition, the restoration of pollen viability, involves a novel two-gene gametophytic system, wherein genes designated Rf3 and Rf4 are required for viability of individual gametes. Rf3 is tightly linked to, or represents, a single gene that regulates a transcript processing activity that cleaves transcriptsof orf107, a chimeric mitochondrial open reading frame specific to IS1112C. The mitochondrial gene urf 209 is also subject to nucleus-specific enhanced transcript processing, 5′ to the gene, conferred by a single dominant gene designated Mmt1. Examinations of transcript patterns in F2 and two backcross populations indicated cosegregation of the augmented orf107 and urf209 processing activities in IS1112C. Several sorghum lines that do not restore fertility or confer orf107 transcript processing do exhibit urf209 transcript processing, indicating that the activities are distinguishable. We conclude that the nuclear gene(s) conferring enhanced orf107 and urf209 processing activities are tightly linked in IS1112C. Alternatively, the similarity in apparent regulatory action of the genes may indicate allelic differences wherein the IS1112C Rf3 allele may differ from alleles of maintainer lines by the capability to regulate both orf107 and urf209 processing activities.

scholarly journals Engineering the AEG1 promoter from cotton to develop male sterile lines

2021 ◽  
Author(s):  
Kamlesh Kumar Soni ◽  
Amita Kush Mehrotra ◽  
Pradeep Kumar Burma
Keyword(s):  
Seed Production ◽  
Reporter Gene ◽  
Tissue Specificity ◽  
Specific Activity ◽  
Open Reading Frame ◽  
Gene Analysis ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Reading Frame ◽  
Regulatory Module

This work reports on modifying the Upstream Regulatory Module (URM, 1.5 Kb region upstream of the open reading frame) of Anther Expressing Gene 1 (AEG1) from cotton to achieve anther specific activity. AEG1 was identified in a previous study aimed to isolate a promoter with tapetum specific activity. Such a promoter could then be used to express barnase and barstar genes for developing male sterile and restorer lines for hybrid seed production in cotton. The AEG1 URM was observed to be active in tapetum as well as in roots making it unusable to drive the expression of barnase gene. Analysis of the URM showed the presence of several root specific motifs. Two modified AEG1 URMs were developed, by removing or mutating these motifs and its activity checked in tobacco. The activity of one of the modified URMs, AEG1(DelBmut) was restricted to the anther tissue as observed using the reporter gene beta-glucuronidase. The study also demonstrates that male sterile lines could be developed in tobacco using the AEG1(DelBmut) URM to express the barnase gene. This work thus shows the possibility of engineering promoters to achieve tissue specificity and to develop male sterile lines in cotton.

scholarly journals Rf8 and Rf* Mediate Unique T-urf13-Transcript Accumulation, Revealing a Conserved Motif Associated With RNA Processing and Restoration of Pollen Fertility in T-Cytoplasm Maize

Genetics ◽  
1997 ◽  
Vol 147 (3) ◽  
pp. 1367-1379 ◽  
Author(s):  
Carren L Dill ◽  
Roger P Wise ◽  
Patrick S Schnable
Keyword(s):  
Pollen Fertility ◽  
Fertility Restoration ◽  
Nuclear Gene ◽  
F1 Hybrids ◽  
Mitochondrial Rna ◽  
Male Sterile ◽  
Transcript Accumulation ◽  
Reading Frame ◽  
Conserved Motif ◽  
Maize Germplasm

Rf8 is a newly described nuclear gene that can substitute for Rf1 to partially restore pollen fertility to male-sterile, T-cytoplasm maize. Families segregating for Rf8 were used to investigate the mechanism of this fertility restoration and to compare it to the restoration conditioned by Rf1. Although Rf8 is unlinked to the rf1 locus, it also alters T-urf13 mitochondrial transcript accumulation and reduces the accumulation of the URF13 protein. Like the 1.6- and 0.6-kilobase (kb) T-urf13 transcripts that accumulate in T-cytoplasm plants carrying Rf1, 1.42- and 0.42-kb transcripts accumulate in plants that are partially restored by Rf8. A survey of T-cytoplasm maize lines, inbreds, and F1 hybrids by mitochondrial RNA gel blot analyses revealed that Rf8, is rare in maize germplasm. These surveys revealed the presence of another rare, weak restorer factor, Rf*, which is uniquely associated with the accumulation of 1.4- and 0.4-kb T-urf13 transcripts. Primer extension analyses position the 5′ termini of the 1.42/0.42-kb and 1.4/0.4-kb transcripts at +137 and +159 nucleotides, respectively, 3′ of the AUG initiation codon of the T-urf13 reading frame. The conserved motif, 5′-CNACNNU-3′, overlaps the 5′ termini of the Rf1-, Rf8-, and Rf*-associated transcripts and the 380 nucleotide, Rf3-associated orf107 transcript from cytoplasmic male sterility sorghum. These results demonstrate that multiple unlinked, nuclear genes can have similar but distinct effects on the expression of the unique T-urf13 mitochondrial coding sequence to restore pollen fertility to T-cytoplasm maize.

Influence of nuclear background on transcription of a chimeric gene (orf256) and coxI in fertile and cytoplasmic male sterile wheats

Genome ◽  
1994 ◽  
Vol 37 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Jiasheng Song ◽  
Charles Hedgcoth
Keyword(s):  
Mitochondrial Dna ◽  
Open Reading Frame ◽  
Mitochondrial Rna ◽  
Coding Region ◽  
Male Sterile ◽  
Reading Frame ◽  
Cytoplasmic Male Sterile ◽  
Triticum Timopheevi ◽  
Nuclear Background ◽  
Flanking Region

Crosses between Triticum timopheevi, as maternal donor, and T. aestivum can lead to cytoplasmic male sterile (cms) plants. The T. timopheevi derived mitochondrial DNA from parental, cms, and fertility-restored lines differs from that of T. aestivum derived mtDNA in the coxI gene region. Our previous results for cms lines showed that there is an open reading frame, orf256, upstream from coxI in T. timopheevi derived mtDNA that is not present in T. aestivum DNA. The 5′ flanking region and the first 33 nucleotides of the coding region of orf256 are identical to the corresponding region of T. aestivum coxI, whereas the rest of orf256, including the 3′ flank, is not related to coxI. Also, the organization of orf256 and coxI on a HindIII fragment from T. timopheevi derived mtDNA are identical in T. timopheevi, cms, and fertility-restored lines. We now report that the DNA sequence of orf256 is identical in T. timopheevi, cms, and fertility-restored lines. Major transcripts in cms and fertility-restored lines encode both orf256 and coxI with 5′ termini like coxI mRNA of T. aestivum, whereas parental mitochondria from T. timopheevi have major transcripts with 5′ termini within the orf256 coding region. Mitochondria from cms and fertility-restored lines have the potential to produce a protein that would not be present in parental T. timopheevi or in T. aestivum.Key words: cytoplasmic male sterility, wheat, mitochondrial DNA, mitochondrial RNA, coxI.

Sign in / Sign up

Export Citation Format

Share Document