scholarly journals Kinetics of enzyme acylation and deacylation in the penicillin acylase-catalyzed synthesis of beta-lactam antibiotics

2003 ◽  
Vol 270 (18) ◽  
pp. 3675-3683 ◽  
Author(s):  
Wynand B. L. Alkema ◽  
Erik de Vries ◽  
Rene Floris ◽  
Dick B. Janssen
Keyword(s):  
Penicillin Acylase ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics ◽  
Kinetics Of

scholarly journals Beta-lactamase of Mycobacterium fortuitum: kinetics of production and relationship with resistance to beta-lactam antibiotics.

1991 ◽  
Vol 35 (9) ◽  
pp. 1760-1764 ◽  
Author(s):  
L Fattorini ◽  
G Scardaci ◽  
S H Jin ◽  
G Amicosante ◽  
N Franceschini ◽  
...  
Keyword(s):  
Mycobacterium Fortuitum ◽  
Beta Lactamase ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics ◽  
Kinetics Of

scholarly journals The kinetics of non-stoichiometric bursts of β-lactam hydrolysis catalysed by class C β-lactamases

1993 ◽  
Vol 295 (1) ◽  
pp. 295-304 ◽  
Author(s):  
M G P Page
Keyword(s):  
Beta Lactam ◽  
Beta Lactamases ◽  
Beta Lactam Antibiotics ◽  
Steady State Rate ◽  
State Rate ◽  
Rate Of Hydrolysis ◽  
Class C ◽  
Burst Kinetics ◽  
Kinetics Of ◽  
Hydrolysis Of

Class C beta-lactamases from Pseudomonas aeruginosa and several species of the Enterobacteriaceae have been observed to undergo a rapid burst in hydrolysis of beta-lactam antibiotics before relaxation to a steady-state rate of hydrolysis. The amplitude of the burst corresponds to the hydrolysis of between 1 and 10,000 mol of the substrate per mol of enzyme. The decay of the rate of hydrolysis in the burst phase comprises two exponential reactions, which indicates that there are three different reactive states of the enzymes. Examination of the kinetics of acylation by slowly reacting beta-lactams suggests that there are three forms of the free enzyme in slow equilibrium. Thus it would appear that the burst kinetics exhibited by class C enzymes can be attributed to redistribution of the enzyme between different conformations induced by the reaction with substrate.

scholarly journals The Kinetics of Ampicillin Release from Hydroxyapatite for Bones Regeneration

2009 ◽  
Vol 2009 ◽  
pp. 1-5
Author(s):  
Giovanilton Ferreira da Silva ◽  
José Sílvio Veras Albuquerque ◽  
Caroliny Gomes de Oliveira ◽  
Ricardo Emilio Ferreira Quevedo Nogueira ◽  
Andrea Lopes de Oliveira Ferreira
Keyword(s):  
Human Medicine ◽  
Beta Lactam ◽  
Adsorption Experiment ◽  
Beta Lactam Antibiotics ◽  
High Release ◽  
Before And After ◽  
Dose Control ◽  
Low Toxicity ◽  
The Difference ◽  
Kinetics Of

Semisynthetic beta-lactam antibiotics are among the most used pharmaceuticals. Their use in veterinary and human medicine is in continuous expansion. There is a growing need for developing bioactive implants. Advantages of implantable drug delivery tools can include high release efficiency, precise dose control, low toxicity, and allow to overcome disadvantages connected with conventional methods. In this respect, hydroxyapatite (HA) is an elective material. It enables to produce architectures similar to those of real bones. Here we studied a kinetic model to describe ampicillin release from HA. In the course of adsorption experiment, ampicillin was dissolved, maintained at and shaken at 60 strokes/minute. Samples were withdrawn periodically for analysis and then returned to the mixture. Adsorbed amounts were measured by the difference of the concentration of the antibiotics before and after adsorption using UV adsorption at 225 nm. The aim of this work was to evaluate its application as ampicillin delivery carrier.

scholarly journals Effect of extracellular water volume on the distribution kinetics of .BETA.-lactam antibiotics as a function of age.

1985 ◽  
Vol 8 (3) ◽  
pp. 167-174 ◽  
Author(s):  
AKIRA TSUJI ◽  
TETSUYA TERASAKI ◽  
NORISHIGE IMAEDA ◽  
KAZUNORI NISHIDE ◽  
EMI NAKASHIMA
Keyword(s):  
Water Volume ◽  
Extracellular Water ◽  
Beta Lactam ◽  
Beta Lactam Antibiotics ◽  
Distribution Kinetics ◽  
Kinetics Of
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