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The general overview aimed to increase the current knowledge interactions between dehydrogenase enzymes
and nanoparticles, and introduce dehydrogenases for industrial and health purposes. Nanoparticles (NPs) are particles
constituting from 1 to 100 nm based on their size with a surrounding interfacial layer. Nanoparticle-Protein interactions
include covalent and non-covalent attachments. Several dehydrogenase enzymes (e.g., alcohol dehydrogenase, lactate
dehydrogenase, alanine dehydrogenase, glutamate dehydrogenase, leucine dehydrogenase, phenylalanine dehydrogenase,
and malate dehydrogenase) are used for immobilization by nanoparticles. Such as magnetic nanoparticles and quantum
dots, represent attractive model systems for biological enzyme assemblies and design of bioanalytical sensors.
Further, bioconjugation of nanoparticles with dehydrogenase enzymes has broad applications in biocatalysis and
nanomedicine for drug discovery. However, studies on the characterization of nanoparticle-enzyme complexes accept
apparent that the anatomy and action of enzymes are afflicted by the chemistry of nanoparticle ligand, size, actual, and
labeling methods.
Moreover, the nanoparticle-protein conjugation revealed increased/decreased enzymatic activity due to nanoparticle
features. Thus, this work reviewed the findings of nanoparticle-enzyme interactions for nanotechnology applications and
conjugation techniques. We also highlight several challenges associated with the nanoparticle-enzyme interactions,
including stability and reusability of the enzymes in nanoparticle-enzyme formation.