scholarly journals Mutational analysis of chitin synthase 2 of Saccharomyces cerevisiae . Identification of additional amino acid residues involved in its catalytic activity

1998 ◽  
Vol 258 (3) ◽  
pp. 941-947 ◽  
Author(s):  
Tomio Yabe ◽  
Toshiko Yamada-Okabe ◽  
Tasuku Nakajima ◽  
Masayuki Sudoh ◽  
Mikio Arisawa ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Catalytic Activity ◽  
Amino Acid ◽  
Mutational Analysis ◽  
Chitin Synthase ◽  
Amino Acid Residues ◽  
Additional Amino Acid

Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity of a Family 18 Chitinase from Tulip Bulbs

2003 ◽  
Vol 67 (2) ◽  
pp. 341-346 ◽  
Author(s):  
Keisuke SUZUKAWA ◽  
Takeshi YAMAGAMI ◽  
Takayuki OHNUMA ◽  
Hideki HIRAKAWA ◽  
Satoru KUHARA ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Mutational Analysis ◽  
Amino Acid Residues ◽  
Tulip Bulbs

scholarly journals Expression and Mutational Analysis of Amino Acid Residues Involved in Catalytic Activity in a Ribonuclease MC1 from the Seeds of Bitter Gourd

2000 ◽  
Vol 64 (3) ◽  
pp. 603-605 ◽  
Author(s):  
Tomoyuki NUMATA ◽  
Tohru KASHIBA ◽  
Madoka HINO ◽  
Gunki FUNATSU ◽  
Masatune ISHIGURO ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Mutational Analysis ◽  
Bitter Gourd ◽  
Amino Acid Residues

scholarly journals Cell surface anchorage and ligand-binding domains of the Saccharomyces cerevisiae cell adhesion protein alpha-agglutinin, a member of the immunoglobulin superfamily.

1993 ◽  
Vol 13 (4) ◽  
pp. 2554-2563 ◽  
Author(s):  
D Wojciechowicz ◽  
C F Lu ◽  
J Kurjan ◽  
P N Lipke
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Adhesion ◽  
Amino Acid ◽  
Cell Surface ◽  
Consensus Sequence ◽  
Binding Domain ◽  
Immunoglobulin Superfamily ◽  
Cell Contact ◽  
Amino Acid Residues ◽  
Adhesion Proteins

alpha-Agglutinin is a cell adhesion glycoprotein expressed on the cell wall of Saccharomyces cerevisiae alpha cells. Binding of alpha-agglutinin to its ligand a-agglutinin, expressed by a cells, mediates cell-cell contact during mating. Analysis of truncations of the 650-amino-acid alpha-agglutinin structural gene AG alpha 1 delineated functional domains of alpha-agglutinin. Removal of the C-terminal hydrophobic sequence allowed efficient secretion of the protein and loss of cell surface attachment. This cell surface anchorage domain was necessary for linkage to a glycosyl phosphatidylinositol anchor. A construct expressing the N-terminal 350 amino acid residues retained full a-agglutinin-binding activity, localizing the binding domain to the N-terminal portion of alpha-agglutinin. A 278-residue N-terminal peptide was inactive; therefore, the binding domain includes residues between 278 and 350. The segment of alpha-agglutinin between amino acid residues 217 and 308 showed significant structural and sequence similarity to a consensus sequence for immunoglobulin superfamily variable-type domains. The similarity of the alpha-agglutinin-binding domain to mammalian cell adhesion proteins suggests that this structure is a highly conserved feature of adhesion proteins in diverse eukaryotes.

scholarly journals Identification of amino acid residues essential for catalytic activity of gentisate 1,2-dioxygenase fromPseudomonas alcaligenesNCIB 9867

2001 ◽  
Vol 204 (1) ◽  
pp. 141-146 ◽  
Author(s):  
Chi Hung Chua ◽  
Yongmei Feng ◽  
Chew Chieng Yeo ◽  
Hoon Eng Khoo ◽  
Chit Laa Poh
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Amino Acid Residues

Analysis of essential amino acid residues for catalytic activity of cis-epoxysuccinate hydrolase from Bordetella sp. BK-52

2013 ◽  
Vol 98 (4) ◽  
pp. 1641-1649 ◽  
Author(s):  
Wenna Bao ◽  
Haifeng Pan ◽  
Zhenhong Zhang ◽  
Yongqing Cheng ◽  
Zhipeng Xie ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Amino Acid ◽  
Essential Amino Acid ◽  
Amino Acid Residues

scholarly journals Identification by Mutational Analysis of Amino Acid Residues Essential in the Chaperone Function of Calreticulin

2005 ◽  
Vol 281 (4) ◽  
pp. 2338-2346 ◽  
Author(s):  
Virginie Martin ◽  
Jody Groenendyk ◽  
Simone S. Steiner ◽  
Lei Guo ◽  
Monika Dabrowska ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mutational Analysis ◽  
Amino Acid Residues ◽  
Chaperone Function

Mutational analysis of positively charged amino acid residues of Uukuniemi phlebovirus nucleocapsid protein

2012 ◽  
Vol 167 (1) ◽  
pp. 118-123 ◽  
Author(s):  
Anna Katz ◽  
Alexander N. Freiberg ◽  
Vera Backström ◽  
Liisa Holm ◽  
Antti Vaheri ◽  
...  
Keyword(s):  
Amino Acid ◽  
Nucleocapsid Protein ◽  
Mutational Analysis ◽  
Amino Acid Residues ◽  
Positively Charged
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