scholarly journals Purification and characterization of fetal bovine serum beta-N-acetyl-D-galactosaminyltransferase and beta-D-glucuronyltransferase involved in chondroitin sulfate biosynthesis

1999 ◽  
Vol 264 (2) ◽  
pp. 461-467 ◽  
Author(s):  
Kazunori Tsuchida ◽  
Thomas Lind ◽  
Hiroshi Kitagawa ◽  
Ulf Lindahl ◽  
Kazuyuki Sugahara ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Purification And Characterization ◽  
Fetal Bovine

Characterization of osteoblastic properties of 7F2 and UMR-106 cultures after acclimation to reduced levels of fetal bovine serum

2008 ◽  
Vol 86 (7) ◽  
pp. 403-415 ◽  
Author(s):  
S. Ganguly ◽  
L.A. Ashley ◽  
C.M. Pendleton ◽  
R.D. Grey ◽  
G.C. Howard ◽  
...  
Keyword(s):  
Cell Lines ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Phenol Red ◽  
Ph Indicator ◽  
Fetal Bovine ◽  
Experimental Findings ◽  
Umr 106 ◽  
Culture Protocol

Estrogen plays an important role in skeletal physiology by maintaining a remodeling balance between the activity of osteoblasts and osteoclasts. In an attempt to decipher the mechanism through which estrogen elicits its action on osteoblasts, experimentation necessitated the development of a culturing environment reduced in estrogenic compounds. The selected medium (OPTI-MEM) is enriched to sustain cultures under reduced fetal bovine serum (FBS) conditions and is devoid of the pH indicator phenol red, a suspected estrogenic agent. This protocol reduced the concentration of FBS supplementation to 0% through successive 24 h incubations with diminishing amounts of total FBS (1%, 0.1%, and 0%). The protocol does not appear to alter the viability, cell morphology, or osteoblast-like phenotype of 7F2 and UMR-106 cell lines when compared with control cells grown in various concentrations of FBS. Although the rate of mitotic divisions declined, the 7F2 and UMR-106 cultures continued to express osteoblast-specific markers and exhibited estrogen responsiveness. These experimental findings demonstrate that the culture protocol developed did not alter the osteoblast nature of the cell lines and provides a model system to study estrogen’s antiresorptive role on skeletal turnover.

Proteomic and functional characterization of endogenous adiponectin purified from fetal bovine serum

PROTEOMICS ◽  
2004 ◽  
Vol 4 (12) ◽  
pp. 3933-3942 ◽  
Author(s):  
Yu Wang ◽  
Gang Lu ◽  
Winifred P. S. Wong ◽  
Johannes F. G. Vliegenthart ◽  
Gerrit J. Gerwig ◽  
...  
Keyword(s):  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Functional Characterization ◽  
Fetal Bovine
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