Genetic structure in Atlantic brown trout (Salmo truttaL.) populations in the Iberian peninsula: evidence from mitochondrial and nuclear DNA analysis

2000 ◽  
Vol 117 (3) ◽  
pp. 105-120 ◽  
Author(s):  
S. Dunner ◽  
L. Royo ◽  
J. Cañon
Keyword(s):  
Genetic Structure ◽  
Iberian Peninsula ◽  
Brown Trout ◽  
Dna Analysis ◽  
Nuclear Dna

scholarly journals Characterization of the genetic structure of the brown trout (Salmo trutta) from “Braduljica” fish farm, Serbia

2019 ◽  
Vol 35 (3) ◽  
pp. 289-299
Author(s):  
Nikola Molerovic ◽  
Bozidar Raskovic ◽  
Radica Djedovic ◽  
Ostojic Andric ◽  
Zoran Markovic ◽  
...  
Keyword(s):  
Genetic Structure ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Nuclear Dna ◽  
Fish Farm ◽  
Dehydrogenase Gene ◽  
Pcr Rflp ◽  
Brown Trout Salmo Trutta ◽  
Broodstock Management

Due to the ecological concerns and preservation of genetic resources, the characterization of genetic structure of the brown trout (Salmo trutta) was carried out on the "Braduljica" fish farm. DNA was isolated from fin clips of 10 individuals, and after that molecular PCR-RFLP methods were used for distinguishing between Atlantic and Danubian lineages based on control region of the mitochondrial DNA (CR mtDNA) and lactate dehydrogenase gene of the nuclear DNA (LDH nDNA). Based on phenotypic characteristics it was estimated that out of 10 individuals included in this study five belonged to the allochtonous Atlantic lineage and remaining five belonged to the native Danubian lineage of brown trout. However, results of molecular analyses showed a high percentage of allochthonous genes among the individuals, which confirms the hybridization between these two lineages. Also, the results showed that the selection based on the phenotype is not adequate. In order to continue with proper broodstock management, it is necessary to eliminate allochtonous individuals of the Atlantic lineage from the broodstock.

scholarly journals Geographic Structure of Mitochondrial and Nuclear Gene Polymorphisms in Australian Green Turtle Populations and Male-Biased Gene Flow

Genetics ◽  
1997 ◽  
Vol 147 (4) ◽  
pp. 1843-1854 ◽  
Author(s):  
Nancy N FitzSimmons ◽  
Craig Moritz ◽  
Colin J Limpus ◽  
Lisa Pope ◽  
Robert Prince
Keyword(s):  
Gene Flow ◽  
Genetic Structure ◽  
Green Turtle ◽  
Nuclear Dna ◽  
Nuclear Gene ◽  
Chelonia Mydas ◽  
Single Copy ◽  
Microsatellite Data ◽  
Ecological Data ◽  
Infinite Allele Model

Abstract The genetic structure of green turtle (Chelonia mydas) rookeries located around the Australian coast was assessed by (1) comparing the structure found within and among geographic regions, (2) comparing microsatellite loci vs. restriction fragment length polymorphism analyses of anonymous single copy nuclear DNA (ascnDNA) loci, and (3) comparing the structure found at nuclear DNA markers to that of previously analyzed mitochondrial (mtDNA) control region sequences. Significant genetic structure was observed over all regions at both sets of nuclear markers, though the microsatellite data provided greater resolution in identifying significant genetic differences in pairwise tests between regions. Inferences about population structure and migration rates from the microsatellite data varied depending on whether statistics were based on the stepwise mutation or infinite allele model, with the latter being more congruent with geography. Estimated rates of gene flow were generally higher than expected for nuclear DNA (nDNA) in comparison to mtDNA, and this difference was most pronounced in comparisons between the northern and southern Great Barrier Reef (GBR). The genetic data combined with results from physical tagging studies indicate that the lack of nuclear gene divergence through the GBR is likely due to the migration of sGBR turtles through the courtship area of the nGBR population, rather than male-biased dispersal. This example highlights the value of combining comparative studies of molecular variation with ecological data to infer population processes.

scholarly journals Analysis of ancient DNA from a prehistoric Amerindian cemetery

1999 ◽  
Vol 354 (1379) ◽  
pp. 153-159 ◽  
Author(s):  
Anne C. Stone ◽  
Mark Stoneking
Keyword(s):  
Ancient Dna ◽  
Dna Analysis ◽  
Nuclear Dna ◽  
Genetic Research ◽  
Hypervariable Region ◽  
Dna Preservation ◽  
European Contact ◽  
Novel Method ◽  
High Level ◽  
Central Illinois

The Norris Farms No. 36 cemetery in central Illinois has been the subject of considerable archaeological and genetic research. Both mitochondrial DNA (mtDNA) and nuclear DNA have been examined in this 700–year–old population. DNA preservation at the site was good, with about 70% of the samples producing mtDNA results and approximately 15% yielding nuclear DNA data. All four of the major Amerindian mtDNA haplogroups were found, in addition to a fifth haplogroup. Sequences of the first hypervariable region of the mtDNA control region revealed a high level of diversity in the Norris Farms population and confirmed that the fifth haplogroup associates with Mongolian sequences and hence is probably authentic. Other than a possible reduction in the number of rare mtDNA lineages in many populations, it does not appear as if European contact significantly altered patterns of Amerindian mtDNA variation, despite the large decrease in population size that occurred. For nuclear DNA analysis, a novel method for DNA–based sex identification that uses nucleotide differences between the X and Y copies of the amelogenin gene was developed and applied successfully in approximately 20 individuals. Despite the well–known problems of poor DNA preservation and the ever–present possibility of contamination with modern DNA, genetic analysis of the Norris Farms No. 36 population demonstrates that ancient DNA can be a fruitful source of new insights into prehistoric populations.

Unidirectional Natural Hybridization between Brown Trout (Salmo trutta) and Atlantic Salmon (S. salar) in Newfoundland

1992 ◽  
Vol 49 (9) ◽  
pp. 1953-1958 ◽  
Author(s):  
Colin McGowan ◽  
William S. Davidson
Keyword(s):  
Atlantic Salmon ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Dna Analysis ◽  
Juvenile Fish ◽  
Natural Hybridization ◽  
Major Influence ◽  
Isolating Mechanisms ◽  
Brown Trout Salmo Trutta ◽  
Sexually Mature

Protein electrophoresis and mitochondrial DNA analysis were used to detect the frequency and direction of natural hybridization between brown trout (Salmo trutta) and Atlantic salmon (S. salar) in nine Newfoundland rivers. In total, 37 hybrids were discovered in a sample of 792 juvenile fish for a regional frequency of 4.67%. Local frequencies ranged from 0.00 to 18.75% and were significantly heterogeneous. All of the hybrids sampled were produced from matings between female brown trout and male Atlantic salmon. Possible reasons for the breakdown of prereproductive isolating mechanisms between these species are considered. Reproductive characteristics of the populations involved appear to have a major influence on the dynamics of hybridization between these species in Newfoundland. It is proposed that an abundance of sexually mature Atlantic salmon parr in Newfoundland streams is responsible for both the frequency and direction of hybridization observed in this study.

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