Blockade of the NMDA receptor increases developmental apoptotic elimination of granule neurons and activates caspases in the rat cerebellum

2000 ◽  
Vol 12 (9) ◽  
pp. 3117-3123 ◽  
Author(s):  
Barbara Monti ◽  
Antonio Contestabile
Keyword(s):  
Nmda Receptor ◽  
Granule Neurons ◽  
Rat Cerebellum

DNA CONTENTS IN PURKINJE CELLS AND INNER GRANULE NEURONS IN THE DEVELOPING RAT CEREBELLUM

1974 ◽  
Vol 16 (3) ◽  
pp. 205-211 ◽  
Author(s):  
SETSUYA FUJITA ◽  
TAKANORI HATTORI ◽  
MASARU FUKUDA ◽  
TADAHISA KITAMURA
Keyword(s):  
Purkinje Cells ◽  
Granule Neurons ◽  
Rat Cerebellum ◽  
Dna Contents ◽  
Developing Rat

scholarly journals Domoic Acid-Induced Neurotoxicity Is Mainly Mediated by the AMPA/KA Receptor: Comparison between Immature and Mature Primary Cultures of Neurons and Glial Cells from Rat Cerebellum

2011 ◽  
Vol 2011 ◽  
pp. 1-14 ◽  
Author(s):  
Helena T. Hogberg ◽  
Anna K. Bal-Price
Keyword(s):  
Nmda Receptor ◽  
Glial Cells ◽  
Domoic Acid ◽  
Primary Cultures ◽  
Mrna Levels ◽  
Neuronal Markers ◽  
Rat Cerebellum ◽  
Increased Sensitivity ◽  
Shellfish Toxin ◽  
Brain Gaba

Domoic acid (DomA) is a naturally occurring shellfish toxin that can induce brain damage in mammalians. Neonates have shown increased sensitivity to DomA-induced toxicity, and prenatal exposure has been associated with e.g. decreased brain GABA levels, and increased glutamate levels. Here, we evaluated DomA-induced toxicity in immature and mature primary cultures of neurons and glial cells from rat cerebellum by measuring the mRNA levels of selected genes. Moreover, we assessed if the induced toxicity was mediated by the activation of the AMPA/KA and/or the NMDA receptor. The expression of all studied neuronal markers was affected after DomA exposure in both immature and mature cultures. However, the mature cultures seemed to be more sensitive to the treatment, as the effects were observed at lower concentrations and at earlier time points than for the immature cultures. The DomA effects were completely prevented by the antagonist of the AMPA/KA receptor (NBQX), while the antagonist of the NMDA receptor (APV) partly blocked the DomA-induced effects. Interestingly, the DomA-induced effect was also partly prevented by the neurotransmitter GABA. DomA exposure also affected the mRNA levels of the astrocytic markers in mature cultures. These DomA-induced effects were reduced by the addition of NBQX, APV, and GABA.

scholarly journals Decreased Expression and Functionality of NMDA Receptor Complexes Persist in the CA1, but Not in the Dentate Gyrus after Transient Cerebral Ischemia

1998 ◽  
Vol 18 (7) ◽  
pp. 768-775 ◽  
Author(s):  
Jee Ching Hsu ◽  
Yu Zhang ◽  
Norio Takagi ◽  
James W. Gurd ◽  
M. Christopher Wallace ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Nmda Receptor ◽  
Dentate Gyrus ◽  
Pyramidal Neurons ◽  
Transient Cerebral Ischemia ◽  
Granule Cell Layer ◽  
Mrna Levels ◽  
Granule Neurons ◽  
Dentate Granule Cell ◽  
Receptor Complexes

The authors investigated the gene expression of the NR2A and NR2B subunits of N-methyl-D-aspartate (NMDA) receptor and the functional electrophysiologic activity of NMDA receptor complexes in the vulnerable CA1 and less vulnerable dentate gyrus subfields of the rat hippocampus at different times after transient cerebral ischemia. Decreased expression for both subtypes was observed in both the CA1 subfield and dentate granule cell layer at early times after challenge; however, the decreased expression in the dentate granule cell layer was reversible because mRNA levels for both the NR2A and NR2B subtypes recovered to, or surpassed, sham-operated mRNA levels by 3 days postchallenge. No recovery of expression for either subtype was observed in the CA1 subfield. The functional activity of NMDA receptor complexes, as assessed by slow field excitatory postsynaptic potentiations (slow f-EPSP) in CA1 pyramidal neurons, was maintained at 6 hours postchallenge; however, this activity was diminished greatly by 24 hours postchallenge, and absent at 7 days postchallenge. A similar pattern was observed for the non-NMDA receptor-mediated fast f-EPSP. In dentate granule neurons, however, no significant change in NMDA receptor-mediated slow f-EPSP from sham control was observed at any time after insult. The non-NMDA receptor-generated fast f-EPSPs also were maintained at all times postinsult in the dentate gyrus. These results illustrate that the activity of NMDA receptors remains functional in dentate granule neurons, but not in the pyramidal neurons of the CA1 subfield, at early and intermediate times after transient cerebral ischemia, and suggest that there is a differential effect of ischemia on the glutamatergic transmission systems in these two hippocampal subfields.

The mechanism of the neurocytotoxic effect of the Na+/H+ exchange inhibitor 5-(N-ethyl-N-isopropyl)-amiloride (EIPA) in the rat cerebellum cultured granule neurons

2014 ◽  
Vol 8 (2) ◽  
pp. 121-124
Author(s):  
E. V. Stelmashook ◽  
S. V. Novikova ◽  
G. A. Amelkina ◽  
E. E. Genrikhs ◽  
L. G. Khaspekov ◽  
...  
Keyword(s):  
Granule Neurons ◽  
Rat Cerebellum ◽  
Exchange Inhibitor
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