In situ hybridization and immunohistochemistry of bone sialoprotein and secreted phosphoprotein 1 (osteopontin) in the developing mouse mandibular condylar cartilage compared with limb bud cartilage

The mouse genes En-1 and En-2 display sequence similarity, in and around the homeobox region, to the engrailed family in Drosophila. This paper describes their pattern of expression in the 12.5-day mouse embryo as determined by in situ hybridization. En-2 is expressed in a subset of cells expressing En-1. Both genes are expressed in the developing midbrain and its junction with the hindbrain. In addition, En-1 is expressed in the floor of the hindbrain, a restricted ventrolateral segment of the neural tube throughout the trunk and anterior part of the tail, the dermatome of tail somites, the centrum and costal processes in developing vertebrae, a restricted region of facial mesenchyme and the limb-bud ectoderm. Supplementary studies of 9.5-day and 10.5-day embryos showed that the same pattern of expression pertained in the neural tube, but that expression in the somites is at first confined to the dermatome and later found at a low level in restricted sclerotomal regions. Both genes are expressed in restricted domains which do not cross tissue-type boundaries. In several instances, however, boundaries of expression lie within morphologically undifferentiated tissue. These results suggest that En-1 and En-2 may be involved in the establishment or maintenance of the spatial integrity of specific domains within developing tissues.

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In situ hybridization reveals differential spatial distribution of mRNAs for type I and type II collagen in the chick limb bud

Development ◽

10.1242/dev.103.1.111 ◽

1988 ◽

Vol 103 (1) ◽

pp. 111-118 ◽

Cited By ~ 1

Author(s):

C.J. Devlin ◽

P.M. Brickell ◽

E.R. Taylor ◽

A. Hornbruch ◽

R.K. Craig ◽

...

Keyword(s):

In Situ Hybridization ◽

Limb Development ◽

Type I Collagen ◽

Type Ii Collagen ◽

Limb Bud ◽

Type I ◽

Type Ii ◽

Mrna Accumulation ◽

Rna Probes

During limb development, type I collagen disappears from the region where cartilage develops and synthesis of type II collagen, which is characteristic of cartilage, begins. In situ hybridization using antisense RNA probes was used to investigate the spatial localization of type I and type II collagen mRNAs. The distribution of the mRNA for type II collagen corresponded well with the pattern of type II collagen synthesis, suggesting control at the level of transcription and mRNA accumulation. In contrast, the pattern of mRNA for type I collagen remained more or less uniform and did not correspond with the synthesis of the protein, suggesting control primarily at the level of translation or of RNA processing.

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mRNA expression patterns of the IGF system during mouse limb bud development, determined by whole mount in situ hybridization

Molecular and Cellular Endocrinology ◽

10.1016/s0303-7207(98)00007-0 ◽

1998 ◽

Vol 138 (1-2) ◽

pp. 151-161 ◽

Cited By ~ 22

Author(s):

Marjolein van Kleffens ◽

Cora Groffen ◽

Roberto R. Rosato ◽

Stefan M. van den Eijnde ◽

Johan W. van Neck ◽

...

Keyword(s):

In Situ Hybridization ◽

Mrna Expression ◽

Expression Patterns ◽

Limb Bud ◽

Bud Development ◽

Igf System ◽

Whole Mount ◽

Mouse Limb

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In Situ Hybridization Analysis of the Expression of the Type II Collagen Gene in the Developing Chicken Limb Bud

Collagen and Related Research ◽

10.1016/s0174-173x(88)80001-3 ◽

1988 ◽

Vol 8 (4) ◽

pp. 277-294 ◽

Cited By ~ 65

Author(s):

Hyun-Duck Nah ◽

Barbara J. Rodgers ◽

William M. Kulyk ◽

Barbara E. Kream ◽

Robert A. Kosher ◽

...

Keyword(s):

In Situ Hybridization ◽

Type Ii Collagen ◽

Hybridization Analysis ◽

Limb Bud ◽

Collagen Gene ◽

Type Ii

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Novel Golgi Protein, GoPro49, Is a Specific Dental Follicle Marker

Journal of Dental Research ◽

10.1177/0022034509338452 ◽

2009 ◽

Vol 88 (6) ◽

pp. 534-538 ◽

Cited By ~ 11

Author(s):

M.S. Takatalo ◽

M. Tummers ◽

I. Thesleff ◽

R. Rönnholm

Keyword(s):

In Situ Hybridization ◽

Periodontal Ligament ◽

Secretory Pathway ◽

Bone Sialoprotein ◽

Follicle Cells ◽

Specific Marker ◽

Dental Follicle ◽

Root Sheath ◽

Golgi Protein

GoPro49 is a recently identified, novel Golgi protein that is expressed in embryonic mesenchymal tissues, including dental follicle. In the present study, we have tested the hypothesis that the gene is a specific marker for the dental follicle, and examined its expression during the development of mouse incisors and molars. In situ hybridization showed that GoPro49 is expressed in dental follicles from bud to post-eruption stages. The expression is intense throughout the dental follicle during crown development, and persists in the root follicle during root development. In the forming periodontal ligament, GoPro49 expression is down-regulated upon differentiation of the follicle cells to cementoblasts and osteoblasts marked by Bsp1. In cultured dental follicle cells, the GoPro49 protein co-localizes with β-COP, suggesting that GoPro49 may function in the secretory pathway. We conclude that GoPro49 is a novel, specific marker for the dental follicle and can be used to identify this tissue. Abbreviations: Bsp1, bone sialoprotein 1; GoPro49, Golgi protein 49 kDa; E16, embryonic day 16; HERS, Hertwig’s epithelial root sheath; PDL, periodontal ligament; dpn, day post-natal.

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