Comparison of a range of green fluorescent protein-tagging vectors for monitoring a microbial inoculant in soil

2001 ◽  
Vol 32 (1) ◽  
pp. 26-30 ◽  
Author(s):  
C.E. Dandie ◽  
S.M. Thomas ◽  
N.C. McClure
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Microbial Inoculant ◽  
Green Fluorescent Protein Tagging ◽  
Green Fluorescent ◽  
Protein Tagging

scholarly journals Quantitative Imaging of B1 Cyclin Expression Across the Cell Cycle Using Green Fluorescent Protein Tagging and Epifluorescence

2020 ◽  
Vol 97 (10) ◽  
pp. 1066-1072
Author(s):  
Arnica Karuna ◽  
Francesco Masia ◽  
Sally Chappell ◽  
Rachel Errington ◽  
Andrew M. Hartley ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Quantitative Imaging ◽  
Green Fluorescent Protein Tagging ◽  
Green Fluorescent ◽  
Protein Tagging

scholarly journals Construction and Application of Epitope- and Green Fluorescent Protein-Tagging Integration Vectors for Bacillus subtilis

2002 ◽  
Vol 68 (5) ◽  
pp. 2624-2628 ◽  
Author(s):  
Marcus Kaltwasser ◽  
Thomas Wiegert ◽  
Wolfgang Schumann
Keyword(s):  
Bacillus Subtilis ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Pcr Amplification ◽  
Epitope Tag ◽  
Inner Membrane Protein ◽  
Green Fluorescent Protein Tagging ◽  
Green Fluorescent ◽  
Protein Tagging

ABSTRACT Here we describe the construction and application of six new tagging vectors allowing the fusion of two different types of tagging sequences, epitope and localization tags, to any Bacillus subtilis protein. These vectors are based on the backbone of pMUTIN2 and replace the lacZ gene with tagging sequences. Fusion of the tagging sequences occurs by PCR amplification of the 3′ terminal part of the gene of interest (about 300 bp), insertion into the tagging vector in such a way that a fusion protein will be synthesized upon integration of the whole vector via homologous recombination with the chromosomal gene. Three of these tagging sequences (FLAG, hemagglutinin, and c-Myc) allow the covalent addition of a short epitope tag and thereby detection of the fusion proteins in immunoblots, while three other tags (green fluorescent protein+, yellow fluorescent protein, and cyan fluorescent protein) are helpful in assigning proteins within one of the compartments of the cell. The versatility of these vectors was demonstrated by fusing these tags to the cytoplasmically located HtpG and the inner membrane protein FtsH.

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