scholarly journals Pollution Characteristics of Typical Antibiotic Resistance Genes in Sludge Treatment Wetlands

2020 ◽  
Vol 194 ◽  
pp. 04010
Author(s):  
Zhuma Luosang ◽  
Wanjun Zhang ◽  
Junwen Ma ◽  
fengying Huang ◽  
Yubo Cui
Keyword(s):  
Antibiotic Resistance ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Bottom Layer ◽  
Treatment Wetlands ◽  
Sludge Treatment ◽  
Pollution Characteristics ◽  
Box Method

The in-situ static box method was used to conduct a comparative experiment on sludge treatment wetlands (STWs). STW1 had ventilation structure, without reeds and STW2 had ventilation and was planted with reeds. The absolute abundance of sulfonamide, tetracycline and macrolide antibiotic resistance genes (ARGs) in the two STWs were analyzed, and the paper discussed pollution characteristics of typical antibiotic resistance genes in the two STWs. The results showed that three ARGs, sul1 (sulfonamides), tetC (tetracyclines), ermf (macrolides), were detected in STW1 and STW2. The concentration of arginine was sul1 > tetC > ermf. The concentration level of AGRS in STW1 and STW2 was lower in bottom layer than that of surface layer sludge. The removal efficiency of ARGs in the same system was tetC > sul1 > ermf, and the removal efficiency of surface sludge and bottom sludge in different systems was STW2 > STW1. Planting wetland plants in the STW can promote the removal of ARGs.

scholarly journals Constructed Wetland Revealed Efficient Sulfamethoxazole Removal but Enhanced the Spread of Antibiotic Resistance Genes

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 834 ◽  
Author(s):  
Shuai Zhang ◽  
Yu-Xiang Lu ◽  
Jia-Jie Zhang ◽  
Shuai Liu ◽  
Hai-Liang Song ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Surface Layer ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Bacterial Communities ◽  
Antibiotic Resistance Genes ◽  
Bottom Layer ◽  
Synthetic Wastewater ◽  
High Level ◽  
Very High

Constructed wetlands (CWs) could achieve high removal efficiency of antibiotics, but probably stimulate the spread of antibiotic resistance genes (ARGs). In this study, four CWs were established to treat synthetic wastewater containing sulfamethoxazole (SMX). SMX elimination efficiencies, SMX degradation mechanisms, dynamic fates of ARGs, and bacterial communities were evaluated during the treatment period (360 day). Throughout the whole study, the concentration of SMX in the effluent gradually increased (p < 0.05), but in general, the removal efficiency of SMX remained at a very high level (>98%). In addition, the concentration of SMX in the bottom layer was higher compared with that in the surface layer. The main byproducts of SMX degradation were found to be 4-amino benzene sulfinic acid, 3-amino-5-methylisoxazole, benzenethiol, and 3-hydroxybutan-1-aminium. Temporally speaking, an obvious increase of sul genes was observed, along with the increase of SMX concentration in the bottom and middle layers of CWs. Spatially speaking, the concentration of sul genes increased from the surface layer to the bottom layer.

scholarly journals Linking the Resistome and Plasmidome to the Microbiome

2018 ◽  
Author(s):  
Thibault Stalder ◽  
Max Press ◽  
Shawn Sullivan ◽  
Ivan Liachko ◽  
Eva M. Top
Keyword(s):  
Antibiotic Resistance ◽  
Resistance Genes ◽  
Antibiotic Resistance Genes ◽  
Wastewater Sample ◽  
Proximity Ligation ◽  
Rapid Spread ◽  
Ligation Method ◽  
Novel Associations

AbstractThe rapid spread of antibiotic resistance is a serious human health threat. A range of environments have been identified as reservoirs of the antibiotic resistance genes (ARGs) found in pathogens. However, we lack understanding of the origins of these ARGs and their spread from environment to clinic. This is partly due to our inability to identify the bacterial hosts of ARGs and the mobile genetic elements that mediate this spread, such as plasmids and integrons. Here we demonstrated that the in vivo proximity ligation method Hi-C can determine the in situ host range of ARGs, plasmids, and integrons in a wastewater sample by physically linking them to their host chromosomes. Hi-C detected both previously known and novel associations between ARGs, mobile elements and host genomes, mostly validating this method. A better identification of the natural carriers of ARGs will aid the development of strategies to limit resistance spread to pathogens.

Removal of bacterial cells, antibiotic resistance genes and integrase genes by on-site hospital wastewater treatment plants: surveillance of treated hospital effluent quality

2017 ◽  
Vol 3 (2) ◽  
pp. 293-303 ◽  
Author(s):  
Kenda Timraz ◽  
Yanghui Xiong ◽  
Hamed Al Qarni ◽  
Pei-Ying Hong
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Removal Efficiency ◽  
Resistance Genes ◽  
Wastewater Treatment Plants ◽  
Antibiotic Resistance Genes ◽  
Bacterial Cells ◽  
Hospital Wastewater ◽  
Effluent Quality ◽  
Microbial Contaminants

This study aims to evaluate the removal efficiency of microbial contaminants by wastewater treatment plants (WWTPs) operated on-site of two hospitals.

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