scholarly journals Dynamics of glutathione reductase activity in rat liver tissues during cryodestruction of the right atrium

2021 ◽  
Vol 273 ◽  
pp. 02014
Author(s):  
Olga Pavlova ◽  
Olga Gulenko ◽  
Konstantin Krupin ◽  
Pavel Boriskin ◽  
Victor Leonov
Keyword(s):  
Oxidative Stress ◽  
Glutathione Reductase ◽  
Rat Liver ◽  
Right Atrium ◽  
Liver Tissue ◽  
Reductase Activity ◽  
The Body ◽  
Glutathione Reductase Activity ◽  
The Right ◽  
Liver Tissues

The metabolic processes of the human body are based on multiple redox reactions and oxidative stress occurs when homeostasis is imbalanced. Antioxidant system of the body is represented by such enzymes as catalase, glutathione reductase, superoxidismutase and glutathione peroxidase. Objective: to study the dynamics of glutathione reductase activity in rat liver tissues after cryodestruction of right atrial myocardium to initiate oxidative stress. Materials and methods: 420 male rats were used. The rats were divided into two groups - intact and experimental, 210 animals in each. To initiate oxidative stress, the experimental group rats underwent cryodestruction of the right atrium. The activity of glutathione reductase in the liver tissue was determined by accumulation of oxidized glutathione before the experiment, as well as on 1, 3, 5, 7 and 14 days of the experiment. Conclusions: oxidative stress arising after cryodestruction of the right atrium up to the 7th day of the experiment provokes a decrease in the glutathione reductase activity in the rat liver tissue, but the start of reparative processes helps to restore the disturbed redox equilibrium in the body and normalize the enzyme level.

scholarly journals Effects of Myosmine on Antioxidative Defence in Rat Liver

2012 ◽  
Vol 63 (1) ◽  
pp. 7-14 ◽  
Author(s):  
Rumyana Simeonova ◽  
Vessela Vitcheva ◽  
Galina Gorneva ◽  
Mitka Mitcheva
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Wistar Rats ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Hepatic Function ◽  
Positive Control ◽  
The Other ◽  
Antioxidative Defence ◽  
Glutathione Reductase Activity

Effects of Myosmine on Antioxidative Defence in Rat LiverMyosmine [3-(1-pyrrolin-2-yl) pyridine] is an alkaloid structurally similar to nicotine, which is known to induce oxidative stress. In this study we investigated the effects of myosmine on enzymatic and non-enzymatic antioxidative defence in rat liver. Wistar rats received a single i.p. injection of 19 mg kg-1 of myosmine and an oral dose of 190 mg kg-1 by gavage. Nicotine was used as a positive control. Through either route of administration, myosmine altered the hepatic function by decreasing the levels of reduced glutathione, superoxide dismutase, and glutathione peroxidase activities on one hand and by increasing malondialdehyde, catalase, and glutathione reductase activity on the other. Compared to control, both routes caused significant lipid peroxidation in the liver and altered hepatic enzymatic and non-enzymatic antioxidative defences. The pro-oxidant effects of myosmine were comparable with those of nicotine.

Analysis of Glutathione-Dependent Enzyme Activities in two Different Rat Hepatomas and in Normal Liver in Relation to their Role in Resistance to Oxidative Stress

1988 ◽  
Vol 74 (6) ◽  
pp. 617-621 ◽  
Author(s):  
Maria Armida Rossi ◽  
Mario Umberto Dianzani
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Reductase Activity ◽  
Antioxidant Properties ◽  
Liver Microsomes ◽  
Normal Liver ◽  
Lipid Hydroperoxides ◽  
Glutathione S Transferases ◽  
Glutathione Reductase Activity

The importance of some glutathione metabolic pathways was examined in two highly dedifferentiated hepatomas, Yoshida AH-130 and Morris 3924 A hepatomas, and in normal liver in relation to their role against oxidative stress. The cytosol prepared from Yoshida hepatoma cells decreased the peroxidation rate in normal liver microsomes and mitochondria, but this antioxidant property was not displayed by Morris hepatoma. Glutathione peroxidase and glutathione-S-transferases activities were extremely low in both hepatomas; glutathione reductase activity values were about half the normal liver values. The large decrease in glutathione peroxidase and glutathione-S-transferases suggests that in these two tumors only small amounts of GSH can be used in reduction or conjugation reactions, such as the reduction of hydrogen peroxide and lipid hydroperoxides or the conjugation of GSH with the end products of lipoperoxidation, aldehydes or ketones. The hypothesis of a more efficient GSSG reduction in hepatomas, due to the low glutathione peroxidase/glutathione reductase activity ratio, is also discussed. The described changes in glutathione related enzymes do not seem to have any correlation with the protective effect against the lipoperoxidative processes displayed by some tumors since these enzymatic activities were similar in both hepatomas whereas only Yoshida hepatoma showed antioxidant properties.

scholarly journals Integrated evaluation of oxidation-reduction processes in rat liver tissues on the background of a mechanical eye injury

2021 ◽  
Author(s):  
О. N. Pavlova ◽  
O. N. Gulenko ◽  
E. S. Korovina ◽  
V. V. Zaytsev
Keyword(s):  
Oxidative Stress ◽  
Rat Liver ◽  
Catalase Activity ◽  
Antioxidant Status ◽  
The Body ◽  
Male Rats ◽  
Mechanical Trauma ◽  
Sod Activity ◽  
Integral Assessment ◽  
Liver Tissues

As a result of mechanical trauma to the eye and damage to the blood-ophthalmic barrier, an inflammatory process occurs and, as a result, oxidative stress is a state of the body that develops against the background of an overproduction of free radicals, with a violation of the effectiveness of antioxidant protection. The aim of the study was to study the dynamics of oxidative stress coefficients for the integral assessment of the antioxidant status of rat liver tissues under oxidative stress induced by mechanical eye trauma. The study was carried out on outbred white sexually mature healthy male rats of six months of age, weighing 220–240 g in the amount of 150 pieces. For the integral assessment of oxidative homeostasis in rats, the coefficients of oxidative stress were used: a coefficient expressing the ratio of catalase activity to SOD activity; antioxidant-prooxidant index (API), which expresses the ratio of catalase activity to MDA concentration; the coefficient of the ratio of the concentration of MDA to the concentration of DC and the local antioxidant index (LAI), which is the ratio of the product of the activities of catalase and SOD to the concentration of MDA. The study found that the antioxidant status of rat liver tissue under oxidative stress caused by mechanical trauma of the eye is most effectively stabilized by standard therapy of mechanical trauma of the eye with the addition of quercetin in the form of injections. 

scholarly journals Glutathione Reductase Activity in Serum and Liver Tissue of Human and Rat with Hepatic Damage

1975 ◽  
Vol 116 (2) ◽  
pp. 127-132 ◽  
Author(s):  
HIROKATSU KUMATA ◽  
KAZUO WAKUI ◽  
HIROSHI SUZUKI ◽  
TAKEHISA SUGAWARA ◽  
INSU LIM ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Liver Tissue ◽  
Reductase Activity ◽  
Hepatic Damage ◽  
Glutathione Reductase Activity

Glutathione reductase activity following re-aeration of hypoxically pretreated wheat roots

1994 ◽  
Vol 102 ◽  
pp. 413-417
Author(s):  
G. Albrecht ◽  
E.-M. Wiedenroth
Keyword(s):  
Oxidative Stress ◽  
Glutathione Reductase ◽  
Reduced Glutathione ◽  
Reductase Activity ◽  
Thiobarbituric Acid ◽  
Wheat Roots ◽  
Reactive Material ◽  
Glutathione Reductase Activity ◽  
High Level ◽  
Root Tissues

SynopsisDuring the first 2 h of oxygen re-exposure, the GSH level was almost constant, while the GSSG increased about 10-fold. This results in a decline of the GSH/GSSG ratio, which reflects oxidative stress induced by re-aeration following hypoxic pretreatment. Further evidence for this is an increase in lipid peroxidation measured as thiobarbituric acid-reactive material (TBA-rm) and the affected content of sulfydryl-groups in the root tissues.In spite of the high level of reduced glutathione in the roots under hypoxia-inducing conditions, they contained a retarded glutathione reductase (GR) activity compared with aerobically grown roots. Re-aeration up to 2 h resulted in a further decrease in GR activity. Only at the end of the 16-h period of re-aeration the enzyme activity was able to recover, by overshooting slightly those values of the continuously aerated controls. This was accompanied by a restoring a high GSH/GSSG ratio and an enhanced level of GSH.

scholarly journals The effect of age and sex on glutathione reductase and glutathione peroxidase activities and on aerobic glutathione oxidation in rat liver homogenates

1969 ◽  
Vol 112 (1) ◽  
pp. 109-115 ◽  
Author(s):  
R E Pinto ◽  
W Bartley
Keyword(s):  
Glutathione Peroxidase ◽  
Glutathione Reductase ◽  
Rat Liver ◽  
Peroxidase Activity ◽  
Redox State ◽  
Reductase Activity ◽  
Glutathione Peroxidase Activity ◽  
Glutathione Reductase Activity ◽  
Liver Homogenates ◽  
Age And Sex

1. Changes in liver glutathione reductase and glutathione peroxidase activities in relation to age and sex of rats were measured. Oxidation of GSH was correlated with glutathione peroxidase activity. 2. Glutathione reductase activity in foetal rat liver was about 65% of the adult value. It increased to a value slightly higher than the adult one at about 2–3 days, decreased until about 16 days and then rose after weaning to a maximum at about 31 days, finally reaching adult values at about 45 days old. 3. Weaning rats on to an artificial rat-milk diet prevented the rise in glutathione reductase activity associated with weaning on to the usual diet high in carbohydrate. 4. In male rats glutathione peroxidase activity in the liver increased steadily up to adult values. There were no differences between male and female rats until sexual maturity, when, in females, the activity increased abruptly to an adult value that was about 80% higher than that in males. 5. The rate of GSH oxidation in rat liver homogenates increased steadily from 3 days until maturity, when the rate of oxidation was about 50% higher in female than in male liver. 6. In the liver a positive correlation between glutathione peroxidase activity and GSH oxidation was found. 7. It is suggested that the coupled oxidation–reduction through glutathione reductase and glutathione peroxidase is important for determining the redox state of glutathione and of NADP, and also for controlling the degradation of hydroperoxides. 8. Changes in glutathione reductase and glutathione peroxidase activities are discussed in relation to the redox state of glutathione and NADP and to their effects on the concentration of free CoA in rat liver and its possible action on ketogenesis and lipogenesis.

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