THE ROLE OF INSOLUBLE PARTICULATES IN THE ICE CRYSTAL FORMATION IN A SUPERCOOLED WATER CLOUD

1987 ◽  
Vol 48 (C1) ◽  
pp. C1-369-C1-374
Author(s):  
H. ANDRIAMBELOMA ◽  
R. MONTMORY ◽  
J. PODZIMEK
Keyword(s):  
Supercooled Water ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Water Cloud ◽  
Ice Crystal Formation

The “KIMWIPE” Effect in Ultra-Thin Cryosections

1985 ◽  
Vol 43 ◽  
pp. 458-459
Author(s):  
I. Taylor ◽  
P. Ingram ◽  
J.R. Sommer
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Muscle Fibers ◽  
Ice Crystals ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Thin Sections ◽  
Skeletal Muscle Fibers ◽  
Freeze Dried ◽  
Ice Crystal Formation

In studying quick-frozen single intact skeletal muscle fibers for structural and microchemical alterations that occur milliseconds, and fractions thereof, after electrical stimulation, we have developed a method to compare, directly, ice crystal formation in freeze-substituted thin sections adjacent to all, and beneath the last, freeze-dried cryosections. We have observed images in the cryosections that to our knowledge have not been published heretofore (Figs.1-4). The main features are that isolated, sometimes large regions of the sections appear hazy and have much less contrast than adjacent regions. Sometimes within the hazy regions there are smaller areas that appear crinkled and have much more contrast. We have also observed that while the hazy areas remain still, the regions of higher contrast visibly contract in the beam, often causing tears in the sections that are clearly not caused by ice crystals (Fig.3, arrows).

scholarly journals Crystal-plane-dependent effects of antifreeze glycoprotein impurity for ice growth dynamics

2019 ◽  
Vol 377 (2146) ◽  
pp. 20180393 ◽  
Author(s):  
Yoshinori Furukawa ◽  
Ken Nagashima ◽  
Shunichi Nakatsubo ◽  
Salvador Zepeda ◽  
Ken-ichiro Murata ◽  
...  
Keyword(s):  
Crystal Growth ◽  
Growth Rates ◽  
Space Station ◽  
Supercooled Water ◽  
Normal Growth ◽  
Crystal Plane ◽  
Impurity Effect ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Antifreeze Glycoprotein

An impurity effect on ice crystal growth in supercooled water is an important subject in relation to ice crystal formation in various conditions in the Earth's cryosphere regions. In this review, we consider antifreeze glycoprotein molecules as an impurity. These molecules are well known as functional molecules for controlling ice crystal growth by their adsorption on growing ice/water interfaces. Experiments on free growth of ice crystals in supercooled water containing an antifreeze protein were conducted on the ground and in the International Space Station, and the normal growth rates for the main crystallographic faces of ice, namely, basal and prismatic faces, were precisely measured as functions of growth conditions and time. The crystal-plane-dependent functions of AFGP molecules for ice crystal growth were clearly shown. Based on the magnitude relationship for normal growth rates among basal, prismatic and pyramidal faces, we explain the formation of a dodecahedral external shape of an ice crystal in relation to the key principle governing the growth of polyhedral crystals. Finally, we emphasize that the crystal-plane dependence of the function of antifreeze proteins on ice crystal growth relates to the freezing prevention of living organisms in sub-zero temperature conditions. This article is part of the theme issue ‘The physics and chemistry of ice: scaffolding across scales, from the viability of life to the formation of planets’.

scholarly journals Effects of freshness on thawing drip and ice crystal formation in frozen spotted mackerel Scomber australasicus

2015 ◽  
Vol 81 (1) ◽  
pp. 124-129 ◽  
Author(s):  
KANAKO HASHIMOTO ◽  
TOKIFUSA KAWASHIMA ◽  
NOBUYUKI YOSHINO ◽  
TAKAAKI SHIRAI ◽  
AKIHIDE TAKIGUCHI
Keyword(s):  
Crystal Formation ◽  
Ice Crystal ◽  
Ice Crystal Formation

scholarly journals FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

1972 ◽  
Vol 53 (1) ◽  
pp. 116-126 ◽  
Author(s):  
Helmut Plattner ◽  
Walter M. Fischer ◽  
Werner W. Schmitt ◽  
Luis Bachmann
Keyword(s):  
Cell Damage ◽  
Crystal Formation ◽  
Ice Crystal ◽  
Unicellular Organisms ◽  
Freeze Etching ◽  
Ice Crystal Formation

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed. In many instances the resultant morphology differed significantly from that obtained from glycerol-treated, freeze-etched cells. Furthermore, viability studies of spray-frozen Chlorella compared favorably with cells frozen by other methods.

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