scholarly journals Molecular identification of Wolbachia and Sodalis glossinidius in the midgut of Glossina fuscipes quanzensis from the Democratic Republic of Congo

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 5 ◽  
Author(s):  
Gustave Simo ◽  
Sartrien Tagueu Kanté ◽  
Joule Madinga ◽  
Ginette Kame ◽  
Oumarou Farikou ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Bacterial Flora ◽  
Tsetse Flies ◽  
Infection Rates ◽  
Republic Of Congo ◽  
African Trypanosomes ◽  
Sodalis Glossinidius ◽  
Aldolase Gene

During the last 30 years, investigations on the microbiome of different tsetse species have generated substantial data on the bacterial flora of these cyclical vectors of African trypanosomes, with the overarching goal of improving the control of trypanosomiases. It is in this context that the presence of Wolbachia and Sodalis glossinidius was studied in wild populations of Glossina fuscipes quanzensis from the Democratic Republic of Congo. Tsetse flies were captured with pyramidal traps. Of the 700 Glossina f. quanzensis captured, 360 were dissected and their midguts collected and analyzed. Sodalis glossinidius and Wolbachia were identified by PCR. The Wolbachia-positive samples were genetically characterized with five molecular markers. PCR revealed 84.78% and 15.55% midguts infected by Wolbachia and S. glossinidius, respectively. The infection rates varied according to capture sites. Of the five molecular markers used to characterize Wolbachia, only the fructose bis-phosphate aldolase gene was amplified for about 60% of midguts previously found with Wolbachia infections. The sequencing results confirmed the presence of Wolbachia and revealed the presence of S. glossinidius in the midgut of Glossina f. quanzensis. A low level of midguts were naturally co-infected by both bacteria. The data generated in this study open a framework for investigations aimed at understanding the contribution of these symbiotic microorganisms to the vectorial competence of Glossina fuscipes quanzensis.

MOLECULAR MARKERS ASSOCIATED WITH PLASMODIUM FALCIPARUM RESISTANCE TO SULFADOXINE-PYRIMETHAMINE IN THE DEMOCRATIC REPUBLIC OF CONGO

2006 ◽  
Vol 75 (1) ◽  
pp. 152-154 ◽  
Author(s):  
SANDRA COHUET ◽  
MICHEL VAN HERP ◽  
UMBERTO D’ALESSANDRO ◽  
MARYLINE BONNET ◽  
CHANTAL VAN OVERMEIR ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Molecular Markers ◽  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Republic Of Congo

scholarly journals Assessment of Molecular Markers of Anti-malarial Drug Resistance in Pfk13-propeller, Pfcrt and Pfmdr1 Genes in Plasmodium Falciparum Isolated From Patients Returning for Malaria Retreatment in Democratic Republic of Congo 

2020 ◽  
Author(s):  
Doudou Malekita Yobi ◽  
Nadine K. Kayiba ◽  
Dieudonné M. Mvumbi ◽  
Raphael Boreux ◽  
Pius Z. Kabututu ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Molecular Markers ◽  
Real Time ◽  
Real Time Pcr ◽  
Copy Number ◽  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
First Line ◽  
Pcr Assay ◽  
Republic Of Congo

Abstract BackgroundThe Democratic Republic of Congo (DRC) malaria treatment policy recommends two first-line artemisinin-based combination therapies (ACTs) for the treatment of uncomplicated malaria: Artesunate-amodiaquine (ASAQ) and Artemether-lumefantrine (AL). This study investigated resistance to the ACTs currently in use in DRC through molecular markers in pfk13, pfcrt and pfmdr1 genes in Plasmodium falciparum isolated from patients returning for malaria retreatment. MethodsFrom November 2018 to November 2019, dried blood spots were taken from patients returning to health structures for fever within 28 days after an initial malaria treatment in 6 sentinel sites of National Malaria Control Programme (NMCP) across DRC. The new episode of malaria was first detected by a rapid diagnostic test (RDT) and then confirmed by a real-time PCR assay. Fragments of interest in pfk13 and pfcrt genes were amplified by conventional PCR before sequencing and Pfmdr1 gene copy number was determined by a TaqMan real-time PCR assay. ResultsOut of 474 enrolled patients, 364 (76.8%) were confirmed positive by PCR for P. falciparum. Of the 325 P. falciparum isolates successfully sequenced in pfk13-propeller gene, 7 (2.2%) carried non-synonymous (NS) mutations among which 3 previously reported (N498I, N554K and A557S) and 4 not yet reported (F506L, E507V, D516E and G538S). Of the 335 isolates successfully sequenced in pfcrt gene, 139 (41.5%) harbored the K76T mutation known to be associated with CQ resistance. The SVMNT haplotype associated with resistance to AQ has not been found. None of the isolates carried increased copy number of pfmdr1 gene among the 322 P. falciparum isolates successfully analyzed.ConclusionNo molecular marker known to date as associated with resistance to first-line ACTs in use was detected in P. falciparum isolated in patients returning for retreatment. Regular monitoring through in vivo drug efficacy and molecular studies must continue to ensure the effectiveness of the treatment of malaria in DRC.

The Role of Gender in Agent Banking: Evidence from the Democratic Republic of Congo

2020 ◽  
Author(s):  
Richard Chamboko ◽  
Robert Cull ◽  
Xavier Gine ◽  
Soren Heitmann ◽  
Fabian Reitzug ◽  
...  
Keyword(s):  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Republic Of Congo

Creative Reconstruction: A Systematic Approach to Reinventing Large Cities in Democratic Republic of Congo

2015 ◽  
Vol 8 (1) ◽  
pp. 1485-1497
Author(s):  
Mia Nsokimieno Misilu Eric
Keyword(s):  
Cultural Values ◽  
Democratic Republic ◽  
Democratic Republic Of Congo ◽  
Mineral Resources ◽  
Capital City ◽  
African Countries ◽  
Republic Of Congo ◽  
Large Cities ◽  
Current State ◽  
Legacy Cities

The current state of large cities in Democratic Republic of Congo highlights the necessity of reinventing cities. More than fifty years after the independence, these major cities, like Kinshasa the capital city, are in a state of are in a state of disrepair. They are damaged, dysfunctional, and more vulnerable. Today, these legacy cities do not meet the international requirements of livable cities. Democratic Republic of Congo faces the challenge of rebuilding its cities for sustainability. The movement for independence of African countries enabled the shift from colonial cities to legacy cities. It is important to understand the cultural and ideological foundations of colonial city. Commonly, colonial cities served as purpose-built settlements for the extraction and transport of mineral resources toward Europe. What's required is a creative reconstruction to achieve a desired successful urban change. Creative reconstruction tends to ensure urban transformation in relation with urbanization, by making continuous and healthy communities. Creative reconstruction seems appropriate way of building back cities in harmony with cultural values. The article provides a framework for urban regeneration. The study is based on principle of thinking globally and acting locally in building back better cities.

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