MOLECULAR MARKERS ASSOCIATED WITH PLASMODIUM FALCIPARUM RESISTANCE TO SULFADOXINE-PYRIMETHAMINE IN THE DEMOCRATIC REPUBLIC OF CONGO

2006 ◽  
Vol 75 (1) ◽  
pp. 152-154 ◽  
Author(s):  
SANDRA COHUET ◽  
MICHEL VAN HERP ◽  
UMBERTO D’ALESSANDRO ◽  
MARYLINE BONNET ◽  
CHANTAL VAN OVERMEIR ◽  
...  
2020 ◽  
Author(s):  
Doudou Malekita Yobi ◽  
Nadine K. Kayiba ◽  
Dieudonné M. Mvumbi ◽  
Raphael Boreux ◽  
Pius Z. Kabututu ◽  
...  

Abstract BackgroundThe Democratic Republic of Congo (DRC) malaria treatment policy recommends two first-line artemisinin-based combination therapies (ACTs) for the treatment of uncomplicated malaria: Artesunate-amodiaquine (ASAQ) and Artemether-lumefantrine (AL). This study investigated resistance to the ACTs currently in use in DRC through molecular markers in pfk13, pfcrt and pfmdr1 genes in Plasmodium falciparum isolated from patients returning for malaria retreatment. MethodsFrom November 2018 to November 2019, dried blood spots were taken from patients returning to health structures for fever within 28 days after an initial malaria treatment in 6 sentinel sites of National Malaria Control Programme (NMCP) across DRC. The new episode of malaria was first detected by a rapid diagnostic test (RDT) and then confirmed by a real-time PCR assay. Fragments of interest in pfk13 and pfcrt genes were amplified by conventional PCR before sequencing and Pfmdr1 gene copy number was determined by a TaqMan real-time PCR assay. ResultsOut of 474 enrolled patients, 364 (76.8%) were confirmed positive by PCR for P. falciparum. Of the 325 P. falciparum isolates successfully sequenced in pfk13-propeller gene, 7 (2.2%) carried non-synonymous (NS) mutations among which 3 previously reported (N498I, N554K and A557S) and 4 not yet reported (F506L, E507V, D516E and G538S). Of the 335 isolates successfully sequenced in pfcrt gene, 139 (41.5%) harbored the K76T mutation known to be associated with CQ resistance. The SVMNT haplotype associated with resistance to AQ has not been found. None of the isolates carried increased copy number of pfmdr1 gene among the 322 P. falciparum isolates successfully analyzed.ConclusionNo molecular marker known to date as associated with resistance to first-line ACTs in use was detected in P. falciparum isolated in patients returning for retreatment. Regular monitoring through in vivo drug efficacy and molecular studies must continue to ensure the effectiveness of the treatment of malaria in DRC.


PLoS ONE ◽  
2017 ◽  
Vol 12 (6) ◽  
pp. e0179142 ◽  
Author(s):  
Dieudonné Makaba Mvumbi ◽  
Thierry Lengu Bobanga ◽  
Jean-Marie Ntumba Kayembe ◽  
Georges Lelo Mvumbi ◽  
Hippolyte Nani-Tuma Situakibanza ◽  
...  

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 5 ◽  
Author(s):  
Gustave Simo ◽  
Sartrien Tagueu Kanté ◽  
Joule Madinga ◽  
Ginette Kame ◽  
Oumarou Farikou ◽  
...  

During the last 30 years, investigations on the microbiome of different tsetse species have generated substantial data on the bacterial flora of these cyclical vectors of African trypanosomes, with the overarching goal of improving the control of trypanosomiases. It is in this context that the presence of Wolbachia and Sodalis glossinidius was studied in wild populations of Glossina fuscipes quanzensis from the Democratic Republic of Congo. Tsetse flies were captured with pyramidal traps. Of the 700 Glossina f. quanzensis captured, 360 were dissected and their midguts collected and analyzed. Sodalis glossinidius and Wolbachia were identified by PCR. The Wolbachia-positive samples were genetically characterized with five molecular markers. PCR revealed 84.78% and 15.55% midguts infected by Wolbachia and S. glossinidius, respectively. The infection rates varied according to capture sites. Of the five molecular markers used to characterize Wolbachia, only the fructose bis-phosphate aldolase gene was amplified for about 60% of midguts previously found with Wolbachia infections. The sequencing results confirmed the presence of Wolbachia and revealed the presence of S. glossinidius in the midgut of Glossina f. quanzensis. A low level of midguts were naturally co-infected by both bacteria. The data generated in this study open a framework for investigations aimed at understanding the contribution of these symbiotic microorganisms to the vectorial competence of Glossina fuscipes quanzensis.


PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237791
Author(s):  
Doudou Malekita Yobi ◽  
Nadine Kalenda Kayiba ◽  
Dieudonné Makaba Mvumbi ◽  
Raphael Boreux ◽  
Sebastien Bontems ◽  
...  

2021 ◽  
Author(s):  
Sabin Nundu ◽  
Hiroaki Arima ◽  
Shirley Simpson ◽  
Ben-Yeddy Abel Chitama ◽  
Yannick Bazitama Munyeku ◽  
...  

Abstract Background. Loss of efficacy of malaria diagnostic tests may lead to untreated or mistreated cases, compromising case management and control. There is an increasing reliance on rapid diagnostic tests (RDTs), with the most widely used of these targeting the Plasmodium falciparum histidine-rich protein 2 gene. There are numerous reports of the deletion of this gene in P. falciparum parasites in some populations, rendering them undetectable by PfHRP2 RDTs. We aimed to identify P. falciparum parasites lacking the P. falciparum histidine rich protein 2 and 3 genes isolated from asymptomatic and symptomatic school-age children in Kinshasa, Democratic Republic of Congo. Methods. We assessed the performance of PfHRP2-based RDTs in comparison to microscopy and PCR. PCR was then used to identify parasite isolates lacking pfhrp2/3 genes. Results. Of 462 DNA samples analysed, deletions of the pfhrp2 and pfhrp3 genes were found in only three (2%) samples and one (1%) sample in the RDT positive subgroup, respectively. No parasites lacking the pfhrp2/3 genes were found in the RDT negative subgroup. Conclusion. Plasmodium falciparum histidine-rich protein 2/3 gene deletions are uncommon in the surveyed population, and do not result in diagnostic failure. We encourage the use of rigorous PCR methods to identify pfhrp2/3 gene deletions in order to minimize the overestimation of their prevalence.


Sign in / Sign up

Export Citation Format

Share Document