scholarly journals Genetic Characterization of Toxoplasma gondii from Wild Rodents in Sichuan Province, Southwestern China

2019 ◽  
Author(s):  
XinLei WANG ◽  
Ling DONG ◽  
Li ZHANG ◽  
Yan LV ◽  
Qian LI ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Toxoplasma Gondii ◽  
Nested Pcr ◽  
Genetic Characterization ◽  
Sichuan Province ◽  
Southwestern China ◽  
Wild Rodents ◽  
New Genotype ◽  
Pcr Rflp

Background: Wild rodents are the intermediate hosts of Toxoplasma gondii. The distribution of genetic diversity of T. gondii in wild rodents is of importance to understand the transmission of this parasite. This study aimed to genetically characterize T. gondii isolates from wild rodents in Sichuan province, southwestern China in 2013. Methods: Genomic DNA was extracted from 10 g wild rodents’ brain samples. Semi-nested PCR and multilocous PCR-RFLP technology were performed to examine genetic diversity of T. gondii isolates as described previously. Results: Overall, 181 brain tissues of different wild rodents, including Eothenomys miletus (n=88), Crocidura attenuate (n=9), Rattus rattus sladeni (n=46), Mus musculus Linnaeus (n=6) and R. niviventer (n=32) were tested for T. gondii DNA, respectively. Six of them were positive for the T. gondii B1 gene by semi-nested PCR amplification, 4 showed complete genotyping results for all 11 polymorphic loci (SAG1, SAG2, alt. SAG2, SAG3, BTUB, GRA6, L358, PK1, C22-8, C29-2 and Apico) by PCR-RFLP, determined to represent a potential new genotype (http://toxodb.org/toxo/). Conclusion: These results documented genetic characterization of T. gondii in wild rodents from Sichuan province, and enriched the genetic diversity of T. gondii in China.

Genetic Characterization of Toxoplasma gondii Isolates From Pigs in Southwestern China

2011 ◽  
Vol 97 (6) ◽  
pp. 1193-1195 ◽  
Author(s):  
Peng Zhou ◽  
Xiu-Tao Sun ◽  
Chuang-Cheng Yin ◽  
Jian-Fa Yang ◽  
Zi-Guo Yuan ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Southwestern China ◽  
Toxoplasma Gondii Isolates

scholarly journals Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 36 ◽  
Author(s):  
Weifeng Qian ◽  
Wenchao Yan ◽  
Chaochao Lv ◽  
Rongzhen Bai ◽  
Tianqi Wang
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Neospora Caninum ◽  
Central China ◽  
Occurrence Rate ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Parasite Infections ◽  
Domestic Rabbits

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.

scholarly journals Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165308 ◽  
Author(s):  
Wen-Bin Zheng ◽  
Xiao-Xuan Zhang ◽  
Jian-Gang Ma ◽  
Fa-Cai Li ◽  
Quan Zhao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Northern China ◽  
Neovison Vison ◽  
Pcr Rflp

scholarly journals Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP

2015 ◽  
Vol 8 (1) ◽  
pp. 57 ◽  
Author(s):  
Qiang Miao ◽  
Si-Yang Huang ◽  
Si-Yuan Qin ◽  
Xin Yu ◽  
Yan Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Southwest China ◽  
Genetic Characterization ◽  
Capra Hircus ◽  
Pcr Rflp

scholarly journals Recent aspects on epidemiology, clinical disease, and genetic diversity of Toxoplasma gondii infections in Australasian marsupials

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jitender P. Dubey ◽  
Fernando H. A. Murata ◽  
Camila K. Cerqueira-Cézar ◽  
Oliver C. H. Kwok ◽  
Chunlei Su ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
New Zealand ◽  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Clinical Disease ◽  
Intermediate Hosts ◽  
Source Of Infection ◽  
The Usa ◽  
Free Ranging

Abstract Background Toxoplasma gondii infections are common in humans and animals worldwide. Among all intermediate hosts of T. gondii, captive marsupials from Australia and New Zealand are highly susceptible to clinical toxoplasmosis. However, most free-range marsupials establish chronic T. gondii infection. Infected marsupial meat may serve as a source of T. gondii infection for humans. Differences in mortality patterns in different species of kangaroos and other marsupials are not fully understood. Lifestyle, habitat, and the genotype of T. gondii are predicted to be risk factors. For example, koalas are rarely exposed to T. gondii because they live on treetops whereas wallabies on land are frequently exposed to infection. Methods The present review summarizes worldwide information on the prevalence of clinical and subclinical infections, epidemiology, and genetic diversity of T. gondii infecting Australasian marsupials in their native habitat and among exported animals over the past decade. The role of genetic types of T. gondii and clinical disease is discussed. Results Fatal toxoplasmosis has been diagnosed in captive Australasian marsupials in Argentina, Chile, China, Germany, Hungary, Japan, Spain, Turkey, and the USA. Most deaths occurred because of disseminated toxoplasmosis. Genetic characterization of T. gondii strains isolated from fatal marsupial infections identified Type III as well as atypical, nonclonal genotypes. Fatal toxoplasmosis was also diagnosed in free-ranging wombats (Vombatus ursinus) in Australia. Genetic characterization of DNA amplified directly from host tissues of subclinical culled kangaroos at slaughter identified many mixed-strain infections with both atypical and recombinant genotypes of T. gondii. Conclusions Most Australasian marsupials in their native land, Australia and New Zealand, have high prevalence of T. gondii, and kangaroo meat can be a source of infection for humans if consumed uncooked/undercooked. The genotypes prevalent in kangaroos in Australia and New Zealand were genetically distinct from those isolated or genotyped from most macropods in the USA and other countries. Thus, clinical toxoplasmosis in marsupials imported from Australia is most likely to occur from infections acquired after importation. Graphic abstract

scholarly journals Genetic characterization of Toxoplasma gondii from cats in Yunnan Province, Southwestern China

2014 ◽  
Vol 7 (1) ◽  
pp. 178 ◽  
Author(s):  
Yi-Ming Tian ◽  
Si-Yang Huang ◽  
Qiang Miao ◽  
Hai-Hai Jiang ◽  
Jian-Fa Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Yunnan Province ◽  
Genetic Characterization ◽  
Southwestern China

Prevalence and genetic characterization of Toxoplasma gondii in badgers ( Melogale moschata ) in southern China by PCR-RFLP

2017 ◽  
Vol 52 ◽  
pp. 30-33 ◽  
Author(s):  
Kai Chen ◽  
Si-Yang Huang ◽  
Jin-Lei Wang ◽  
Rong-Liang Hu ◽  
Qiu-Xia Yao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Southern China ◽  
Pcr Rflp

scholarly journals Genetic characterization of Toxoplasma gondii from pigs from different localities in China by PCR-RFLP

2013 ◽  
Vol 6 (1) ◽  
pp. 227 ◽  
Author(s):  
Hai-Hai Jiang ◽  
Si-Yang Huang ◽  
Dong-Hui Zhou ◽  
Xiao-Xuan Zhang ◽  
Chunlei Su ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Pcr Rflp

scholarly journals Isolation and genetic characterization of Toxoplasma gondii from free-ranging and captive birds and mammals in Pernambuco state, Brazil

2018 ◽  
Vol 27 (4) ◽  
pp. 481-487 ◽  
Author(s):  
Marcio André Silva ◽  
Hilda Fátima Jesus Pena ◽  
Herbert Sousa Soares ◽  
Juliana Aizawa ◽  
Solange Oliveira ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Genetic Population ◽  
Wild Mammals ◽  
Genotypic Characterization ◽  
Pcr Rflp ◽  
Dna Fragment ◽  
Free Ranging ◽  
Lontra Longicaudis

Abstract Recent genetic population studies on Toxoplasma gondii in Brazil have shown large genetic variability. The objective of the present study was to isolate and genotypically characterize T. gondii from free-ranging and captive wild mammals and birds in Pernambuco state, Brazil. Fragments of heart, brain, skeletal muscle and diaphragm tissue from 71 birds and 34 mammals, which were either free-ranging or captive, were collected. Samples from 32 of these animals were subjected to bioassays in mice. Samples from the remaining 73 animals underwent biomolecular diagnosis, using PCR technique, targeting a repetitive DNA fragment of 529 bp in T. gondii. A non-virulent isolate (TgButstBrPE1) was obtained from a free-ranging striated heron (Butorides striata) and, based on primary samples, seven animals were found to be positive. The primary samples and the isolate obtained were subjected to PCR-RFLP using the markers SAG1, 5’3’SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico and CS3. ToxoDB-RFLP genotype #13 from the striated heron isolate and Type BrIII genotype from a captive otter ( Lontra longicaudis) (PS-TgLonloBrPE1) were obtained. The present study describes the first isolation and genotypic characterization of T. gondii in free-ranging striated heron, and the first genotypic characterization of T. gondii in a captive otter.

scholarly journals Subtype Characterization and Zoonotic Potential of Cryptosporidium felis in Cats in Guangdong and Shanghai, China

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 89
Author(s):  
Jiayu Li ◽  
Fuxian Yang ◽  
Ruobing Liang ◽  
Sheng Guo ◽  
Yaqiong Guo ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Genetic Characterization ◽  
Zoonotic Potential ◽  
Common Occurrence ◽  
High Genetic Diversity ◽  
Gp60 Gene ◽  
The Common ◽  
Subtyping Tool

Cryptosporidiumfelis is an important cause of feline and human cryptosporidiosis. However, the transmission of this pathogen between humans and cats remains controversial, partially due to a lack of genetic characterization of isolates from cats. The present study was conducted to examine the genetic diversity of C. felis in cats in China and to assess their potential zoonotic transmission. A newly developed subtyping tool based on a sequence analysis of the 60-kDa glycoprotein (gp60) gene was employed to identify the subtypes of 30 cat-derived C. felis isolates from Guangdong and Shanghai. Altogether, 20 C. felis isolates were successfully subtyped. The results of the sequence alignment showed a high genetic diversity, with 13 novel subtypes and 2 known subtypes of the XIXa subtype family being identified. The known subtypes were previously detected in humans, while some of the subtypes formed well-supported subclusters with human-derived subtypes from other countries in a phylogenetic analysis of the gp60 sequences. The results of this study confirmed the high genetic diversity of the XIXa subtype family of C. felis. The common occurrence of this subtype family in both humans and cats suggests that there could be cross-species transmission of C. felis.

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