New purification method for vitamin A derived aging pigments

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).

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The Effect of Vitamin A on the Intermittent Nitrogen Dioxide Gas Exposure in Hamsters

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090865 ◽

1976 ◽

Vol 34 ◽

pp. 176-177

Author(s):

J.C.S. Kim ◽

M.G. Jourden ◽

E.S. Carlisle

Keyword(s):

Electron Microscopy ◽

Vitamin A ◽

Nitrogen Dioxide ◽

Chronic Exposure ◽

Light And Electron Microscopy ◽

Specific Effect ◽

Deficient Diet ◽

Intermittent Exposure ◽

Hypovitaminosis A ◽

Gas Exposure

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.

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Uultrastructure of Microtubules

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094528 ◽

1972 ◽

Vol 30 ◽

pp. 252-253

Author(s):

Ariaki Nagayama

Keyword(s):

Fine Structure ◽

Cultured Cells ◽

Present Report ◽

Confluent Monolayer ◽

Purification Method ◽

Vinca Rosea ◽

L Cells ◽

Antimitotic Activity ◽

Monolayer Cultures ◽

Rapid Purification

Vinblastine(Vb) or vincristine, alkaloid derived from Vinca rosea is known for its antimitotic activity by regrouping of microtubules into paracrystalline form within the cells. A rapid purification method of vinblastine-induced microtubular paracrystals(PC) has provided us with a fresh and pure microtubular material demonstrating the presence of a labile ATPase associated with the PC. The present report is concerned with the fine structure of purified microtubules of mammalian cultured cells.Confluent monolayer cultures of L cells were incubated for 20hrs with 10-5 M Vb (donated from Shionogi Seiyaku & Co., Osaka, Japan).

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