Toxicological effects of functionalized Carbon Black Nanoparticles on Type II pneumocytes

Pneumologie ◽  
2013 ◽  
Vol 67 (12) ◽  
Author(s):  
N Schreiber ◽  
R Hochscheid ◽  
E Kotte ◽  
P Weber ◽  
B Müller
Keyword(s):  
Carbon Black ◽  
Type Ii ◽  
Toxicological Effects ◽  
Type Ii Pneumocytes ◽  
Carbon Black Nanoparticles

The Identification of Phospholipid Micelles in Glutaraldehyde-Urea Embedded Tissue

1975 ◽  
Vol 33 ◽  
pp. 494-495
Author(s):  
Daniel C. Pease
Keyword(s):  
Electron Micrographs ◽  
Lung Tissue ◽  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Type Ii ◽  
Lead Citrate ◽  
Phospholipid Micelles ◽  
Type Ii Pneumocytes ◽  
Ultrathin Sections ◽  
Polar Water

It is reasonable to think that phospholipid micelles should be visible and identifiable in electron micrographs of ultrathin sections if only they can be preserved throughout the embedding process. The development of highly polar, water-containing, aminoplastic embedments has made this a likely possibility. With this in mind, an investigation of the lecithin-secreting, Type II pneumocytes of the lung is underway.Initially it has been easiest to recognize phospholipid micelles in lung tissue fixed first with glutaraldehyde, and then secondarily exposed to osmium tetroxide. However, the latter is not a necessary concomitant for micellar preservation. Conventional uranyl acetate and lead citrate staining is finally applied. Importantly, though, the micelles have been most easily seen in tissue embedded in 507. glutaraldehyde polymerized with urea, as described in detail by D.C. Pease and R.G. Peterson (J. Ultra- struct. Res., 41, 133, 1972). When oriented appropriately, the micellar units are seen as tiny, bilayer plates.

Flow cytometric identification and isolation of hypertrophic type II pneumocytes after partial pneumonectomy

1989 ◽  
Vol 257 (3) ◽  
pp. C528-C536 ◽  
Author(s):  
B. D. Uhal ◽  
S. R. Rannels ◽  
D. E. Rannels
Keyword(s):  
Gradient Centrifugation ◽  
Type Ii Cells ◽  
Type Ii ◽  
Acridine Orange Staining ◽  
Flow Cytometric ◽  
Percoll Density Gradient Centrifugation ◽  
Type Ii Pneumocytes ◽  
The Mean ◽  
Cellular Dna ◽  
The Right

Type II pneumocytes were isolated by either Percoll density gradient centrifugation or by immunoglobulin G (IgG) panning from the lungs of normal rats and the right lung of rats subjected to left pneumonectomy. Cells were studied at 7- (pnx-7) and 15- (pnx-15) days postoperative, times during and after, respectively, rapid compensatory growth of the right lung. Acridine orange staining permitted resolution of type II cells from contaminants on the basis of high red fluorescence (greater than 590 nm). Simultaneous measurement of forward-angle light scatter (FALS) suggested a shift of pnx-7 cells toward greater size, which was reversed in pnx-15 cells. By Percoll gradient isolation, approximately 15% of pnx-7 cells analyzed were above the mean FALS of control cells. In contrast, approximately 30% of the pnx-7 cells isolated by IgG panning were above the mean FALS of corresponding control cells. Biochemical analyses of pnx-7 cells separated by cell sorting into "high FALS" and "low FALS" subgroups revealed that high FALS type II cells contained 50% more protein (P less than 0.05) and 140% more RNA (P less than 0.01) than low FALS cells, with no significant change in cellular DNA content. These data are consistent with previous studies of type II cells isolated from the lungs of pneumonectomized animals and confirm the presence of hypertrophic cells in these preparations. They provide a foundation from which to design further flow cytometric studies of the role of hypertrophic type II pneumocytes in compensatory lung growth.

scholarly journals Pulmonary surfactant secretion in type II pneumocytes in the existence of inflammatory cells

1990 ◽  
Vol 52 ◽  
pp. 184
Author(s):  
Yoshiaki Oda ◽  
Hirofumi Kai ◽  
Kazunori Takaki ◽  
Tae Yasunaga ◽  
Kouichirou Murahara ◽  
...  
Keyword(s):  
Pulmonary Surfactant ◽  
Inflammatory Cells ◽  
Type Ii ◽  
Type Ii Pneumocytes ◽  
Surfactant Secretion

Oxidative peeling of carbon black nanoparticles

RSC Advances ◽  
2015 ◽  
Vol 5 (112) ◽  
pp. 92539-92544 ◽  
Author(s):  
Peter M. Wilson ◽  
François Orange ◽  
Maxime J.-F. Guinel ◽  
Mikhail Shekhirev ◽  
Yang Gao ◽  
...  
Keyword(s):  
Sulfuric Acid ◽  
Carbon Black ◽  
Potassium Permanganate ◽  
Carbon Black Nanoparticles

We demonstrate that layered carbon black nanoparticles can be oxidatively peeledviathe reaction with potassium permanganate in sulfuric acid.

scholarly journals Plutonium-induced Proliferative Lesions and Pulmonary Epithelial Neoplasms in the Rat: Immunohistochemical and Ultrastructural Evidence for Their Origin from Type II Pneumocytes

1994 ◽  
Vol 31 (3) ◽  
pp. 366-374 ◽  
Author(s):  
R. A. Herbert ◽  
B. S. Stegelmeier ◽  
N. A. Gillett ◽  
A. H. Rebar ◽  
W. W. Carlton ◽  
...  
Keyword(s):  
Clara Cell ◽  
Normal Lung ◽  
Type Ii ◽  
Epithelial Hyperplasia ◽  
Cell Antigen ◽  
Alveolar Epithelial ◽  
Type Ii Pneumocytes ◽  
Epithelial Neoplasms ◽  
Epithelial Lesions ◽  
Surfactant Apoprotein

Immunohistochemistry and transmission electron microscopy were used to clarify the cellular origin for plutonium-239-induced pulmonary proliferative (preneoplastic) epithelial lesions and epithelial neoplasms in F344 rats. Examples of each histologic type of proliferative lesion and neoplasm were stained by the avidin-biotin complex immunoperoxidase method using antibodies to rat surfactant apoprotein and Clara cell antigen. Rat surfactant apoprotein immunostaining was detected in type II pneumocytes in sections of normal lung, in the cells of the proliferative lesions classified histologically as alveolar epithelial hyperplasia (51) and mixed foci (alveolar epithelial hyperplasia with fibrosis) (30), and in adenomas (2), adenocarcinomas (3), and adenosquamous carcinomas (2). With the exception of one adenosquamous carcinoma, Clara cell antigen immunostaining was not detected in any of the pulmonary lesions but was detected in nonciliated cuboidal epithelial (Clara) cells in normal bronchioles. The epithelial cells of the proliferative lesions and neoplasms had ultrastructural features consistent with type II pneumocytes, i.e., the presence of cytoplasmic lamellar and multivesicular bodies. The results of these studies indicate that the majority of plutonium-induced proliferative epithelial lesions and neoplasms in the rat originate from alveolar type II pneumocytes.

scholarly journals Comments on Induction of Inflammasome-dependent Pyroptosis by Carbon Black Nanoparticles

2011 ◽  
Vol 286 (38) ◽  
pp. le17-le17 ◽  
Author(s):  
Leonard S. Levy ◽  
Ishrat Chaudhuri ◽  
Peter Morfeld ◽  
Robert McCunney
Keyword(s):  
Carbon Black ◽  
Carbon Black Nanoparticles
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