Isolation of antioxidant response element inducing compounds from Yongdamsagan-Tang by the gradient elution mode of centrifugal partition chromatography

Planta Medica ◽  
2016 ◽  
Vol 81 (S 01) ◽  
pp. S1-S381
Author(s):  
JH Kim ◽  
YM Kim ◽  
CY Kim
Keyword(s):  
Gradient Elution ◽  
Antioxidant Response ◽  
Antioxidant Response Element ◽  
Partition Chromatography ◽  
Centrifugal Partition Chromatography ◽  
Response Element ◽  
Gradient Elution Mode

scholarly journals Application of Centrifugal Partition Chromatography for Bioactivity-Guided Purification of Antioxidant-Response-Element-Inducing Constituents from Atractylodis Rhizoma Alba

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2274 ◽  
Author(s):  
Myeong Kim ◽  
Ji Kim ◽  
Ahmed Syed ◽  
Young-Mi Kim ◽  
Kevin Choe ◽  
...  
Keyword(s):  
Antioxidant Response ◽  
Luciferase Reporter ◽  
Antioxidant Response Element ◽  
Partition Chromatography ◽  
Reporter Assay ◽  
Centrifugal Partition Chromatography ◽  
Response Element ◽  
1H And 13C Nmr ◽  
Chemical Structures ◽  
Atractylenolide I

Activity-guided separation of antioxidant response element (ARE)-inducing constituents from the rhizomes of Atractylodis Rhizoma Alba was performed by the combination of centrifugal partition chromatography (CPC) and an ARE luciferase reporter assay. From 3 g of the active n-hexane fraction, one polyacetylene, (6E,12E)-tetradeca-6,12-dien-8,10-diyne-1,3-diyl diacetate (47.3 mg), and two sesquiterpenes, atractylenolide I (40.9 mg), and selina-4(14),7(11)-dien-8-one (6.0 mg) were successfully isolated by CPC with n-hexane–ethyl acetate–methanol–water (8:2:8:2, v/v). The chemical structures of the isolated compounds were determined by 1H- and 13C-NMR and ESI-MS. Among the isolated compounds, (6E,12E)-tetradeca-6,12-diene-8,10-diyne-1,3-diol diacetate and selina-4(14),7(11)-dien-8-one increased ARE activity 32.9-fold and 16.6-fold, respectively, without significant cytotoxicity, when 5 µM sulforaphane enhanced ARE activity 27.1-fold. However, atractylenolide I did not increase ARE activity at 100 µM, and showed cytotoxicity at concentrations over 10 µM.

scholarly journals Induction of the Protective Antioxidant Response Element Pathway by 6-Hydroxydopamine In Vivo and In Vitro

2005 ◽  
Vol 87 (1) ◽  
pp. 176-186 ◽  
Author(s):  
Rebekah J. Jakel ◽  
Jonathan T. Kern ◽  
Delinda A. Johnson ◽  
Jeffrey A. Johnson
Keyword(s):  
Antioxidant Response ◽  
Antioxidant Response Element ◽  
Response Element ◽  
6 Hydroxydopamine

scholarly journals Homocysteine stimulates antioxidant response element-mediated expression of glutamate-cysteine ligase in mouse macrophages

2009 ◽  
Vol 203 (1) ◽  
pp. 105-111 ◽  
Author(s):  
Florian Bea ◽  
Francesca N. Hudson ◽  
Haley Neff-LaFord ◽  
Collin C. White ◽  
Terrance J. Kavanagh ◽  
...  
Keyword(s):  
Antioxidant Response ◽  
Antioxidant Response Element ◽  
Glutamate Cysteine Ligase ◽  
Response Element ◽  
Mouse Macrophages

scholarly journals Identification of a Functional Antioxidant Response Element within the Eighth Intron of the Human ABCC3 Gene

2014 ◽  
Vol 43 (1) ◽  
pp. 93-99 ◽  
Author(s):  
Mark J. Canet ◽  
Matthew D. Merrell ◽  
Bryan G. Harder ◽  
Jonathan M. Maher ◽  
Tongde Wu ◽  
...  
Keyword(s):  
Antioxidant Response ◽  
Antioxidant Response Element ◽  
Response Element
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