LONG TERM, FREQUENT PLASMA EXCHANGE DONATION OF CRYOPRECIPITATE

1987 ◽  
Author(s):  
B McLeod ◽  
R Sassetti ◽  
E Cole ◽  
P Scott
Keyword(s):  
Factor Viii ◽  
Plasma Exchange ◽  
Good Health ◽  
Complement C3 ◽  
Laboratory Monitoring ◽  
Extensive Experience ◽  
Normal Limits ◽  
Single Donor ◽  
Donation Frequency ◽  
Fresh Plasma

In plasma exchange donation (PED), several liters of fresh plasma are removed fran a donor with a pheresis instrument as a source of cryoprecipitate, and replaced with autologous cryoprecipitate-supernatant from the previous donation. Repetitive PED can produce large quantities of factor VIII from individual donors over time, with a favorable impact on donor exposure for factor VIII recipients. To clarify the implications for donor safety, we report our experience with several donors who have undergone multiple PEDs. Detailed observations are presented for one donor who has undergone PED 101 times between 5/83 and 1/87, and has provided all the factor VIII needed by his son (now age 14) with severe hemophilia A during this period. Exchange volume was gradually increased while donation frequency was gradually decreased. There were 23 exchanges of 2 L, 52 of 2.5 L, and 26 of 3 L for a total of 254 L plasma exchanged. Desmopressin (20 meg tV) was given before 45 more recent donations to augment factor VIII yield. A total of 343,274 IU factor VIII have been collected; the mean (±SD) yield from a 3 L, desmopressin- stimulated PED is 5598 ± 899 IU. The donor has remained in good health; he has noted no adverse effects fran any PED, and none have been found in laboratory monitoring. Prior to the 100th donation the following were within normal limits: CBC,platelet count, urinalysis, SMA-18, protein electrophoresis, IgG, IgA, IgM, hemolytic complement, C3, C4, fibronectin, prothrombin time, partial thromboplastin time, thranbin time, factor VIII:C (140%), factor VIII:Ag (134%), von Willebrand factor (86%) and fibrinogen (215 mg/dL). In another family, the father has donated 40 times since 1981 and the paternal grandmother has donated 31 times since 1984 with no untoward effects detected in clinical or laboratory monitoring. They have supported two moderately affected patients now ages 7 and 9. Extensive experience with these donors suggests that repeated PED is safe, and that a highly motivated donor can sometimes provide single donor support, even for a severe hemophiliac.

Use of 'single donor' factor VIII from plasma exchange donation

JAMA ◽  
1984 ◽  
Vol 252 (19) ◽  
pp. 2726-2729 ◽  
Author(s):  
B. C. McLeod
Keyword(s):  
Factor Viii ◽  
Plasma Exchange ◽  
Single Donor

Improved Factor VIII Yield in Cryoprecipitate Using a Quick Thaw Technique

1975 ◽  
Author(s):  
J. F. Davidson ◽  
J. H. McAdam ◽  
M. J. Mackenzie ◽  
M. L. Kavanagh
Keyword(s):  
Factor Viii ◽  
Standard Method ◽  
Liquid Bath ◽  
Activity Factor ◽  
Factor Viii Antigen ◽  
Fresh Plasma

Standard Cryoprecipitate was prepared from fresh citrate phosphatedextrose plasma by snap freezing at —70° C and then thawing at +4° C in air for 18 hours. In 143 experiments the yield of Factor VIII from the starting plasma was 42%.In 64 paired experiments the Factor VIII yield in Cryoprecipitate from fresh plasma was increased, from 43% in the standard method to 56% when a quick thaw of 50 minutes at +4° C in a liquid bath was introduced. In 10 other paired experiments the yield in the standard method was raised from 51% to 61% when 90 minutes of super-cooling at —6° C in a liquid bath was introduced prior to snap freezing. When, however, the quick thaw and super-cooling modifications were combined in 42 paired experiments, the yield was only 49% compared with 42% by the standard method.It is concluded that this simple quick thaw modification will produce a greater yield of Factor VIII in Cryoprecipitate and that the addition of the technically more demanding super-cooling modification does not give a significantly greater yield.It seems likely that the longer period at +4° C in the standard method leads to denaturation of a proportion of the Factor VIII and loss of activity. Factor VIII antigen, however, was not lost. In a smaller number of experiments approximately all the Factor VIII was recovered in the Cryoprecipitate and its supernatant. Furthermore, the relative proportions of Factor VIII antigen and procoagulant in the Cryoprecipitate were found to vary in concert suggesting that the Factor VIII molecule is not dissociated in the process of cryoprecipitation.

scholarly journals POSSIBILITIES OF ULTRASONIC MONITORING OF ENDOMETRIUM AND OVARIES IN PATIENTS AFTER CANCELLATION OF LONG-TERM RECEPTION OF COMBINED ORAL CONTRACEPTIVES WHEN PLANNING PREGNANCY

2019 ◽  
Vol 6 (4) ◽  
pp. 215-220
Author(s):  
Sh. N Bortsvadze ◽  
Evgeniya A. Svidinskaya ◽  
T. A Dzhibladze ◽  
I. D Khokhlova ◽  
Yan Van
Keyword(s):  
Menstrual Cycle ◽  
Oral Contraceptives ◽  
Ovarian Tissue ◽  
Hormonal Contraception ◽  
Reproductive Function ◽  
Good Health ◽  
Automatic Assessment ◽  
Normal Limits ◽  
Ultrasound Diagnostics

The article aims to assess the possibility of ultrasound scanning and dopplerstudy in the assessment of the condition of the ovaries and endometrial in patients after long intake of COC, predicting the restoration of reproductive function after the abolition of hormonal contraception. Material and methods. In 2018-2019, 37 women were examined after the abolition of oral contraceptives, which they took for a long period of time. At the time of the examination, all the patients stopped taking COC at least 2 months ago due to the fact that they were planning a pregnancy. UW the study was conducted on the apparat Voluson E8 Expert (General Electric), improving the quality of diagnostics used Automatic assessment of follicle condition based on ultrasonic echography (Sono AVCTM follicle) and tomographic ultrasound (TUI). Results. According to the study significant changes recorded 17 patients whose menstrual cycle did not recover within the first 6 months after the cancellation of COC. In a group of 20 patients with a regular menstrual cycle, the results of the study were within normal limits, pregnancy within 6 months occurred on their own in 8 of them. Conclusion. The possibilities of 3 dimensional reconstruction and programs for evaluating the follicular apparatus significantly improve the quality of ultrasound diagnostics, give more information about the presence of small follicles, blood flow in the ovarian tissue, the structure of the cortical apparatus substances and stroma, whichis the ability of a clinician to obtain a complete understanding of the condition of the ovaries and endometrial and predicting a good health of patients. The article may be of interest to obstetricians-gynecologists, ultrasound specialists, endocrinologists, reproductive specialists.

A Method For Rapid Visualization Of The Size- Distribution Of Factor VIII-Related Protein In Plasma

1981 ◽  
Author(s):  
B A Perret ◽  
R Felix ◽  
M Furlan ◽  
E A Beck
Keyword(s):  
Factor Viii ◽  
Size Distribution ◽  
Protein Pattern ◽  
Related Protein ◽  
Step Method ◽  
Silver Stain ◽  
Molecular Weights ◽  
One Step ◽  
Fresh Plasma ◽  
Normal Human

Factor VUI-related protein circulates in normal human plasma as a series of multimeric forms with apparent molecular weights ranging from 1 to 20×106. So far, combined electrophoretic and immunologic methods permitted demonstration of variable concentration and size- distribution of factor VIII-related protein in von Willebrand’s disease as compared to normal. We now have devised a one-step method for determining the size pattern of this plasma protein. Fresh plasma, containing 1% SDS and 0.8M urea, was layered on top of 2.5% polyacrylamide gels with 2.75% by weight of methylene bisacrylamide/acrylamide. Following extended electrophoresis in 0.2% SDS-0.1M Tris/HCl (pH 7.4), the gels were soaked in 5% formaldehyde and then extensively washed with 10% ethanol. Proteins were visualized employing an ultrasensitive ammoniacal silver stain. This staining revealed a multimeric protein pattern in the upper part of the gel the distribution of which was recorded by densitometry. The protein was identified as factor VIII by two-dimensional immunoelectrophoresis. The method was reproducible and allowed densitometric evaluation within 24 hr.

An IGM Circulating Anticoagulant Associated With Small Cell Carcinoma Of The Lung

1981 ◽  
Author(s):  
C R Handorf ◽  
M J Ricardo ◽  
F L White
Keyword(s):  
Factor Viii ◽  
Cell Carcinoma ◽  
Small Cell Carcinoma ◽  
High Titer ◽  
Anticoagulant Activity ◽  
Laboratory Data ◽  
Small Cell ◽  
White Female ◽  
Factor Viii Activity ◽  
Normal Limits

A 62 year old white female with small cell carcinoma of the lung presented with a prolonged activated partial thromboplastin time (APTT). The patient had no history of abnormal bleeding but previously had pulmonary emboli. Coagulation parameters other than APTT were within normal limits. Pertinent laboratory data included an IgM lambda paraprotein. Dilution studies indicated the presence of a high titer circulating anticoagulant; specific assay revealed marked inhibition of factor VIII (50 Bethesda units). Factor VIII activity was 26% of normal. A purified IgM fraction of the patient’s plasma was obtained by filtration on a Bio-Gel A-1.5m column. When incubated with normal plasma the IgM fraction demonstrated anticoagulant activity by prolonging the APTT.The patient underwent plasmapheresis with albumin and plasma exchange which significantly corrected the APTT. The combination of small cell carcinoma of the lung and IgM paraprotein with anti-factor VIII activity represents an unusual paraneoplastic syndrome; to our knowledge this combination has not been reported previously.

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