Students’ interest in physical education learning: Analysis of internal and external factors

In physical education, learning interests are required so that the intentions of this meant learning are well accomplished. This research is aimed to find out the descriptions of students’ interest factors when attending physical education learning at State Senior High School 2 Malang. This study employed a quantitative descriptive research design through survey methods. In order to obtain the research data, a questionnaire was employed as the research instrument with 329 students as the research subjects. After the data were collected, they were analyzed by using a type of descriptive statistical analysis. The results of this research indicated that students’ interest in learning physical education of X class students in State Senior High School 2 Malang both of internal and external factors were classified as high categories. Internal factors influenced students’ interests more during physical education learning compared to external factors. Overall, the activity factor and feeling of pleasure are the most influential factors for their involvement in physical education learning. Understanding that, students and teachers are suggested to look at intervening toward each above factors as an effort to improve their interest in learning.

SENP3 Promotes Bladder Cancer Proliferation and EMT Transformation by Affecting the Expression of PYCR1 via DeSUMOylation of STAT3

Abnormal activation of signal transducer and activator of transcription 3 (STAT3) has been found in various types of human cancers, including bladder cancer (BC). In our study, we examined the regulation of STAT3 post-translational modifications (PTMs) and found that SENP3 is high in bladder cancer. SENP3 and STAT3 were highly expressed in BC tissues when compared with tissue adjacent to carcinoma. SENP3 induced STAT3 protein level and p-STAT3 translocating into nuclear through deSUMOylation of STAT3. Further, nuclear STAT3, as a transcriptional activity factor, promoted pyrroline-5-carboxylate reductase 1 PYCR1 gene and protein level by interacting with the promoter of (PYCR1). Next, we found that knockdown of PYCR1 inhibited Epithelial to mesenchymal transition of bladder cancer, and simultaneously mitigated the carcinogenic effects of STAT3. In vitro, STAT3 knockdown in bladder cancer cells inhibited cell proliferation, migration, and invasion. In contrast, SENP3 overexpression reversed these effects. In all, results lend novel insights into the regulation of STAT3, which has key roles in bladder cancer progression.

Facile Route Fabrication of Steam Exploded Poplar Loaded with Non-metal Doped Ni-Fe Nanoparticles: Catalytic Activities in 4-nitrophenol Reduction and Electrocatalytic Reaction

A simple and effective method for the preparation of non-metallic ion-doped nickel-supported catalyst is reported. Using economical and recyclable fiber raw materials as carriers, nickel-supported catalysts are prepared by adsorption and reduction at room temperature. The nanoparticles dispersedly anchored on a rational support can efficiently inhibit the aggregation and thus enhance the catalytic activity. For the model catalytic hydrogenation of 4-NP by NaBH4, the N-B-Ni2P/SEP and N-B-NiFeP/SEP catalysts exhibited much better catalytic performances than other catalysts recently reported in terms of the catalytic activity (reaction completed within 5 min), reaction rate constant (1.617 and 0.765 min− 1) and the activity factor K (539 and 255 min− 1·g− 1), respectively. The catalyst showed activities for electrocatalytic HER and OER under ambient conditions. In general, the reported preparation method of nickel-supported catalyst is convenient, economical and environmentally friendly, which is in line with many green chemistry and sustainable development principles and widely available starting materials.

Individual calculation of perfusion activity based on ventilation efficiency for V/P-SPECT

Aim Implementation of the individual calculation of perfusion activity to ensure the guideline-compliant ratio of perfusion to ventilation (P/V-ratio) of ≥ 3 in the diagnosis of acute pulmonary embolism (PE) using V/P-SPECT. Material and Methods 50 consecutive V/P-SPECT examinations, in which a standard activity of 160 MBq was applied for perfusion imaging, are evaluated retrospectively. Based on this patient group an activity factor is determined, which provides a correlation between the applied perfusion activity and the expected perfusion counts of the gamma camera. Using the mean activity factor, the perfusion activity required for a P/V-ratio of four is calculated using the previously acquired ventilation count rate. This is applied prospectively to the 100 subsequent examinations. Results The mean perfusion activity factor is (54.56 ± 10.13) cps/MBq. The individually calculated perfusion activities range from 80 MBq to 200 MBq with an average value of (146.9 ± 35.3) MBq and a median of 140 MBq. The individual activity calculation thus reduced the mean perfusion activity by 8.2 % and the median by 12.5 %. In addition, the individual calculation reduced the proportion of P/V ratios < 3 from 14 % to 0 % and the proportion of P/V ratios > 5 from 24 % to 19 %. Conclusion The presented method for the individual calculation of perfusion activity offers a simple way to ensure a guideline-compliant P/V-ratio. Furthermore, unnecessarily high perfusion activity as a result of inadequate ventilation can be avoided.

Sensitivities of isoprene emissions to soil moisture and impacts on surface ozone levels as simulated over the Euro-Mediterranean region by the regional climate model RegCM4.7chem-CLM4.5-MEGAN2.1

&lt;p&gt;In response to changes in environmental conditions (e.g., temperature, radiation, soil moisture), plants emit biogenic volatile organic compounds (BVOCs). In the large family of BVOCs, isoprene dominates and plays an important role in atmospheric chemistry. Once released in the atmosphere, isoprene influences levels of ozone, thus affecting both climate and air quality. In turn, climate change may alter isoprene emissions by increasing the occurrence and intensity of severe thermal and water stresses that alter plant functioning. To better constrain the evolution of isoprene emissions under future climates, it is critical to reduce the uncertainties in global and regional estimates of isoprene under present climate. Part of these uncertainties is related to the impact of water stress on isoprene. Recently, the BVOC emission model MEGAN has adopted a more sophisticated soil moisture activity factor &amp;#947;&lt;sub&gt;sm&lt;/sub&gt; which does not only account, as previously, for soil moisture available to plants but also links isoprene emissions to photosynthesis and plant water stress.&lt;/p&gt;&lt;p&gt;To assess the effects of soil moisture on isoprene emissions and, lastly, on ozone levels in the Euro-Mediterranean region, we use the regional climate model RegCM4.7, coupled to the land surface model CLM4.5, MEGAN2.1 and a chemistry module (RegCM4.7chem-CLM4.5-MEGAN2.1). We have performed a control experiment over 1987-2016 (with a 5-yr spin-up) at a horizontal resolution of 0.22&amp;#176;. Model output from the control experiment is used to initialize RegCM4.7chem-CLM4.5-MEGAN2.1 for the 10 most dry/wet summers (May-August) selected referring to the 1970-2016 precipitation climatology. Each May-August experiment is run with the old and with the new MEGAN soil moisture activity factor &amp;#947;&lt;sub&gt;sm&lt;/sub&gt;.&amp;#160; The results are then compared with a simulation whit no soil moisture activity factor. Both activity factors &amp;#947;&lt;sub&gt;sm&lt;/sub&gt; reduce isoprene emissions under water deficit.&lt;/p&gt;&lt;p&gt;During dry summers, the old soil moisture activity factor reduces isoprene emissions homogeneously over the model domain by nearly 100%, while ozone levels decrease by around 10%. When the new &amp;#947;&lt;sub&gt;sm &lt;/sub&gt;is used,&lt;sub&gt;&lt;/sub&gt;isoprene emissions are reduced with a patchy pattern by 10-20%, while ground-surface ozone levels diminish homogeneously by few percent over the southern part of the model domain.&lt;/p&gt;

Evolutionary insights into coagulation factor IX Padua and other high-specific-activity variants

The high-specific-activity factor IX (FIX) variant Padua (R338L) is the most promising transgene for hemophilia B (HB) gene therapy. Although R338 is strongly conserved in mammalian evolution, amino acid substitutions at this position are underrepresented in HB databases. We therefore undertook a complete 20 amino acid scan and determined the specific activity of human (h) and canine (c) FIX variants with every amino acid substituted at position 338. Notably, we observe that hFIX-R338L is the most active variant and cFIX-R338L is sevenfold higher than wild-type (WT) cFIX. This is consistent with the previous identification of hFIX-R338L as a cause of a rare X-linked thrombophilia risk factor. Moreover, WT hFIX and cFIX are some of the least active variants. We confirmed the increased specific activity relative to FIX-WT in vivo of a new variant, cFIX-R338I, after gene therapy in an HB dog. Last, we screened 232 pediatric subjects with thromboembolic disease without identifying F9 R338 variants. Together these observations suggest a surprising evolutionary pressure to limit FIX activity with WT FIX rather than maximize FIX activity.

Pemodelan Kausal Faktor-Faktor Beban Keluarga dalam Merawat Pasien Kanker Menggunakan Algoritma S2C-Latent

<p class="Abstrak">Pasien kanker memiliki kebutuhan yang kompleks mulai dari masalah fisik, psikologis, sosial dan spiritual. Keluarga yang merawat pasien kanker disebut<em> family car</em><em>egiver</em>. Seorang <em>family caregiver</em> membantu mengatasi hampir semua permasalahan yang dialami pasien baik saat dirawat di rumah maupun di rumah sakit. Keluarga mengalami suka dan duka dalam merawat pasien. Dalam merawat pasien dengan penyakit kronis, bukan hanya pasien tetapi kesejahteraan dan kualitas hidup <em>family car</em><em>egiver</em> juga penting. Oleh karena itu sangat penting untuk mengetahui bagaimana beban family caregiver dan faktor-faktor yang mempengaruhi beban keluarga dalam merawat pasien. Beban <em>family car</em><em>egiver</em> dapat diukur menggunakan <em>Caregiver Reaction Assesment</em> (CRA), yang direpresentasikan oleh beberapa faktor. Dengan memahami hubungan kausal antara faktor-faktor beban keluarga, diharapkan dapat membantu untuk mengidentifikasi bagaimana beban <em>caregiver</em> bersumber dan berdampak. Untuk itu, penelitian ini bertujuan untuk mengidentifikasi hubungan kausal antara faktor-faktor yang berhubungan dengan beban family caregiver dalam merawat pasien. Penelitian ini menggunakan algoritma pemodelan kausal bernama <em>Stable Specification Search for </em><em>Cross-sectional Data with </em><em>Latent </em><em>Variable</em> (S3C-<em>Latent</em>) untuk mendapatkan model kausal antara faktor-faktor beban <em>family car</em><em>egiver</em> yang relevan. Dari hasil analisis pemodelan didapatkan ada 3 faktor yang memiliki hubungan kausal dan 2 faktor memiliki hubungan asosiasi. Gender memiliki hubungan kausal yang stabil terhadap kesiapan kesehatan dan kesiapan dalam merawat<em>.</em> Sedangkan faktor kesiapan merawat mempengaruhi faktor aktivitas <em>family caregiver</em>, selain itu faktor keuangan memiliki hubungan asosiasi yang kuat dengan faktor aktivitas dan hubungan keluarga. Pemodelan kausal ini dapat digunakan sebagai acuan bagi tenaga kesehatan dalam pelayanan kesehatan yang lebih tepat, efisien, dan efektif di dalam menangani permasalahan beban <em>caregiver</em><em>.</em></p><p class="Abstrak"> </p><p class="Abstrak"><em><strong>Abstract</strong></em></p><p class="Abstrak"><em><span lang="IN">Cancer patients have complex needs ranging from physical, psychological, social</span>,<span lang="IN"> and spiritual problems. Families who</span> take<span lang="IN"> care for cancer patients are called family caregivers. A family caregiver helps </span>to <span lang="IN">overcome almost all problems experienced by </span>the <span lang="IN">patients both while being treated at home and in the hospital. Families experience joy and sorrow in caring for patients. In treating patients with chronic diseases, not only the patient but the family caregiver's well-being and quality of life are also important. Therefore, it is very important to know how the family caregiver's burden is and the factors that affect the family burden in caring for patients. Caregiver family burden can be measured using Caregiver Reaction Assessment (CRA), which is represented by several factors. By understanding the causal relationship between family burden factors, it is hoped that it can help to identify how the caregiver burden is sourced and impacted. Therefore, this study aims to identify the causal relationship</span>s<span lang="IN"> between factors related to the burden on family caregivers in caring for patients. This study uses a causal modeling algorithm called Stable Specification Search for Cross-sectional Data with Latent Variable (S3C-Latent) to obtain a causal model between the relevant caregiver family load factors. The results of modeling analysis showed that there are 3 factors </span>which<span lang="IN"> have a causal relationship and 2 factors have an association relationship. Gender has a stable causal relationship to health readiness and readiness to care</span>, Moreover, t<span lang="IN">he caring readiness factor affects the family caregiver activity factor, </span>and the <span lang="IN">financial factor </span>has <span lang="IN">a strong association with the activity factor and family relationships. This causal modeling can be used as a reference for health workers so as to give health services which are precise, efficient, and effective in dealing with caregiver burden problems.</span></em></p>

Structural basis for the multi-activity factor Rad5 in replication stress tolerance

The yeast protein Rad5 and its orthologs in other eukaryotes promote replication stress tolerance and cell survival using their multiple activities, including ubiquitin ligase, replication fork remodeling and DNA lesion targeting activities. Here, we present the crystal structure of a nearly full-length Rad5 protein. The structure shows three distinct, but well-connected, domains required for Rad5’s activities. The spatial arrangement of these domains suggest that different domains can have autonomous activities but also undergo intrinsic coordination. Moreover, our structural, biochemical and cellular studies demonstrate that Rad5’s HIRAN domain mediates interactions with the DNA metabolism maestro factor PCNA and contributes to its poly-ubiquitination, binds to DNA and contributes to the Rad5-catalyzed replication fork regression, defining a new type of HIRAN domains with multiple activities. Our work provides a framework to understand how Rad5 integrates its various activities in replication stress tolerance.