An evaluation of currently available methods for plasma fibrinogen

1979 ◽  
Author(s):  
K Rickard ◽  
J Burridge ◽  
T Exner ◽  
P Power
Keyword(s):  
Ammonium Sulphate ◽  
Fibrinogen Level ◽  
Reference Method ◽  
Test Methods ◽  
Sodium Sulphite ◽  
Plasma Fibrinogen ◽  
Alkaline Solutions ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Plasma Samples

An assessment of fibrinogen concentration is basic to any investigation of clotting dysfunction and often an estimate of fibrinogen level is needed rapidly as an indication of consumptive coagulopathy or fibrinolysis. Fibrinogen levels in a variety of clinical plasma samples were assessed concurrently by several methods. Results were correlated against a reference method based on Ancrod-clottable fibrinogen and calibrated by U.V. absorbance with alkaline solutions of carefully dried fibrin standard. The best correlations with the reference method were achieved by an immunologic method using the Centrifichem principle and by heat precipitation with quantitation by packing in micro-haematocrit tubes. A modified clot opacity method also gave acceptable results. The turbidimetric ammonium sulphate and sodium sulphite precipitation methods correlated less well with the reference method, and in particular the sodium sulphite technique gave high apparent fibrinogen levels with jaundiced plasmas. Neither of the turbidimetric methods were useful for fibrinogen levels below 50mg/dl. The thrombin time method showed excellent sensitivity to fibrinogen, even at very low fibrinogen levels, but did not correlate well with the reference method. This apparently conflicts with the findings of a recent CAP survey which strongly favoured the thrombin time method. We believe there is a danger that such surveys promote test methods on which there is good interlaboratory agreement, but which may not be specific in function.

An Evaluation of Currently Available Methods for Plasma Fibrinogen

1979 ◽  
Author(s):  
K.A. Rickard ◽  
J. Burridge ◽  
T. Exner ◽  
P. Power
Keyword(s):  
Ammonium Sulphate ◽  
Fibrinogen Level ◽  
Reference Method ◽  
Test Methods ◽  
Sodium Sulphite ◽  
Plasma Fibrinogen ◽  
Alkaline Solutions ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Plasma Samples

An assessment of fibrinogen concentration is basic to any investigation of clotting dysfunction and often an estimate of fibrinogen level is needed rapidly as an indication of consumptive coagulopathy or fibrinolysis. Fibrinogen levels in a variety of clinical plasma samples were assessed concurrently by several methods. Results were correlated against a reference method based on Ancrod-clottable fibrinogen and calibrated by U.V. absorbance with alkaline solutions of carefully dried fibrin standard. The best correlations with the reference method were achieved by an immunologic method using the Centrifichem principle and by heat precipitation with quantitation by packing in micro-haematocrit tubes. A modified clot opacity method also gave acceptable results. The turbidimetric ammonium sulphate and sodium sulphite precipitation methods correlated less well with the reference method, and in particular the sodium sulphite technique gave high apparent fibrinogen levels with jaundiced plasmas. Neither of the turbidimeteric methods were useful for fibrinogen levels below 50mg/dl. The thrombin time method showed excellent sensitivity to fibrinogen, even at very low fibrinogen levels, but did not correlate well with the reference method. This apparently conflicts with the findings of a recent CAP survey which strongly favoured the thrombin time method. We believe there is a danger that such surveys promote test methods on which there is good inter-labotatory agreement, but which may not be specific in function.

Fibrinogen measurement in cardiac surgery with cardiopulmonary bypass: Analysis of repeatability and agreement of Clauss method within and between six different laboratories

2014 ◽  
Vol 112 (07) ◽  
pp. 109-117 ◽  
Author(s):  
Ekaterina Baryshnikova ◽  
Armando Tripodi ◽  
Christoph J. Schlimp ◽  
Herbert Schöchl ◽  
Janne Cadamuro ◽  
...  
Keyword(s):  
Cardiac Surgery ◽  
Cardiopulmonary Bypass ◽  
Plasma Fibrinogen ◽  
Detection Methods ◽  
Fibrinogen Concentration ◽  
Plasma Samples ◽  
Limits Of Agreement ◽  
Study Objective ◽  
Standard Operating ◽  
Mean Differences

SummaryPlasma fibrinogen concentration is important for coagulopathy assessment, and is most commonly measured using the Clauss method. Several factors, including device type and reagent, have been shown to affect results. The study objective was to evaluate performance and repeatability of the Clauss method and to assess differences between measurements performed during and after cardiopulmonary bypass (CPB), by testing plasma samples from patients undergoing cardiac surgery with CPB. Samples were collected from 30 patients before surgery, approximately 20 minutes before weaning from CPB, and 5 minutes after CPB and protamine. Fibrinogen concentration was determined using the Clauss method at six quality-controlled specialised laboratories, according to accredited standard operating procedures. Regarding within-centre agreement for Clauss measurement, mean differences between duplicate measurements were between 0.00 g/l and 0.15 g/l, with intervals for 95% limits of agreement for mean Bland-Altman differences up to 1.3 g/l. Regarding between-centre agreement, some mean differences between pairs of centres were above 0.5 g/l. Differences of up to ∼2 g/l were observed with individual samples. Increased variability was observed between centres, with inter-class correlation values below 0.5 suggesting only fair agreement. There were no significant differences in fibrinogen concentration before weaning from CPB and after CPB for most centres and methods. In conclusion, considerable differences exist between Clauss-based plasma fibrinogen measured using different detection methods. Nevertheless, the similarity between measurements shortly before weaning from CPB and after CPB within centres suggests that on-pump measurements could provide an early estimation of fibrinogen deficit after CPB and thus guidance for haemostatic therapy.

Effect of Three Fibrate Derivatives and of Two HMG-CoA Reductase Inhibitors on Plasma Fibrinogen Level in Patients with Primary Hypercholesterolemia

1993 ◽  
Vol 70 (02) ◽  
pp. 241-243 ◽  
Author(s):  
Adriana Branchi ◽  
Angelo Rovellini ◽  
Domenico Sommariva ◽  
Angelo G Gugliandolo ◽  
Angelo Fasoli
Keyword(s):  
Slow Release ◽  
Fibrinogen Level ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Plasma Fibrinogen Level ◽  
Primary Hypercholesterolemia ◽  
Reductase Inhibitors ◽  
Hmg Coa Reductase Inhibitors ◽  
Coa Reductase ◽  
Hypocholesterolemic Drugs

SummaryIn order to evaluate the effects of hypocholesterolemic drugs on plasma fibrinogen concentration, six groups of subjects with primary hypercholesterolemia have been put on treatment with diet alone or diet plus fenofibrate (100 mg t.i.d.), slow release bezafibrate (400 mg once a day), gemfibrozil (600 mg b.i.d.), simvastatin (20 mg once a day) or pravastatin (20 mg once a day) respectively. After 1 month of therapy, plasma fibrinogen significantly decreased by 9% and 15% in fenofibrate and bezafibrate groups respectively and increased by 19% in gemfibrozil treated patients. After 4 months of therapy the changes were −16% with fenofibrate, −10% with bezafibrate and +20% with gemfibrozil. No significant changes were observed in patients treated with diet alone, simvastatin or pravastatin. The fibrinogen lowering effect of fenofibrate and bezafibrate does not seem to be related to the hypolipidemic activity of the drugs.

scholarly journals THE MEASUREMENTS OF BLOOD CLOTTING IN CARDIORENAL SYNDROME IN ELDERLY

2018 ◽  
Vol 17 (3) ◽  
pp. 27-32
Author(s):  
Ya. A. Naumov ◽  
O. P. Shevchenko ◽  
I. Ju. Orlova ◽  
R. A.o. Faradzhov ◽  
N. A. Naumova
Keyword(s):  
Creatinine Level ◽  
Blood Clotting ◽  
Fibrinogen Level ◽  
Cardiorenal Syndrome ◽  
The Elderly ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Kidney Dysfunction ◽  
Platelet Number ◽  
Group 3

Aim. To assess the changes in hemocoagulation parameters according to the grade of renal dysfunction in elderly patients with cardiorenal syndrome types II and IV.Material and methods. In 56 patients of the elderly age group (mean age 78±10 y.o.), with coronary heart disease and chronic heart failure, the parameters of blood clotting were assessed and the relations with kidney dysfunction grade measured by the level of glomerular filtration rate (GFR). Patients were selected to 3 groups. In 47 (83,9%) there was decline of GFR <60 mL/min/1,73 m2, of those in 8 (17,3%) <30 mL/min/1,73 m2; in 9 GFR >60 mL/min/1,73 m2, with no signs of proteinuria (16,1%). All patients underwent coagulological assessment of the blood with measurement of the activated partial thromboplastin time, thrombin time and prothrombin time, international normalized ratio and fibrinogen concentration, as the complete blood count with platelet number, mean platelet volume, thrombocyte distribution width, and thrombocrit.Results. The number of platelets did differ significantly in groups 1 and 3 (p=0,040), as 2 and 3 (p=0,007). Thrombocrit values did differ significantly only in 2 and 3 (p=0,029). In the group 3 the rate of the mentioned values was below the respective reference values. Fibrinogen levels did differ significantly in groups 1 and 3 (p=0,042), and 2 and 3 (p=0,037). In the group 3 the parameter was higher than upper limit of reference range. Correlation found for GFR and fibrinogen level (r=-0,425; p=0,004), for GFR and platelet number (r=0,271; p=0,049). The platelet number correlated with creatinine level (ρ=-0,392; p=0,004). Creatinine level also correlated with fibrinogen level (ρ=0,375; p=0,012).Conclusion. In the elderly, with the decline of GFR there is decline of thrombocyte number and increase of fibrinogen concentration together with an increase of the severity of kidney dysfunction. The pathological changes that were found might probably serve as additional factor influencing the risk of adverse events related to disorder of blood clotting. The importance of the revealed relations, as the aimfulness for clinical application, should be evaluated in controlled studies.

Fibrinocoagulopathy in Maturity Onset Diabetes Mellitus and Atherosclerosis

1973 ◽  
Vol 30 (01) ◽  
pp. 123-132 ◽  
Author(s):  
R.N Banerjee ◽  
A.L Sahni ◽  
Vijay Kumar
Keyword(s):  
Diabetes Mellitus ◽  
Half Life ◽  
Juvenile Diabetes ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Maturity Onset Diabetes ◽  
Human Fibrinogen ◽  
Fibrinogen Degradation Product ◽  
Onset Diabetes

SummaryImmunofluorescent demonstrations of platelet-free fibrin deposit in the micro and macro-vascular lesions of maturity onset diabetes mellitus and atherosclerosis by earlier workers called for investigations of fibrino-coagulopathy in the two disorders. The present investigation included the determinations of plasma thrombin time (PTCT), fibrinogen concentration (FC), plasma fibrinogen half life (PFT ½) and plasma fibrinogen degradation product (FDP) in (i) the subjects of the two age-onset disorders and their consanguinous family members, and (ii) juvenile diabetes.The assays were carried out by standard methods. 125Iodine labelled human fibrinogen was used for half life studies.In a study of 525 subjects, very highly significant (p <0.001) reduction in PTCT, FC and PFT ½ with a very highly significant (p < 0.001) elevation of FDP were observed in the patients of the two age onset disorders. The results were normal in juvenile diabetes.The study provides convincing evidence of a ‘state of slow consumptive fibrino-coagulopathy’ in the subjects and their siblings with the observed haemostatic defect likely being due to genetically determined Anti-thrombin III deficiency. This observation stimulates a unitary concept of a primary fibrinopathic vascular aetio-pathogenesis, with selective localisation in the macro and microvascular compartment being responsible for the clinical syndromes of ischaemic and metabolic alterations in athersclerosis and maturity onset diabetes mellitus respectively.

The Relationship of Plasma Fibrinogen, Erythrocyte Flexibility and Blood Viscosity

1977 ◽  
Vol 38 (03) ◽  
pp. 0660-0667 ◽  
Author(s):  
P. A Dupont ◽  
J. A Sirs
Keyword(s):  
Blood Viscosity ◽  
Fibrinogen Level ◽  
Plasma Viscosity ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Plasma Fibrinogen Level ◽  
Shear Rates ◽  
High Shear Rates ◽  
Direct Measurements ◽  
Relationship Of

SummaryMeasurements have been made of plasma fibrinogen concentration, erythrocyte flexibility and blood viscosity at shear rates from 5.75 to 230 sec−1 during and following surgery. In the post-operative period the plasma fibrinogen level in the patient rose to over 1,000 mg/dl and because there were subsequent complications, only returned to normal after 4 weeks. There was an associated change of erythrocyte flexibility, with a correlation coefficient of 0.98. The blood viscosity also varied with the plasma fibrinogen level, the effect being more pronounced at low shear rates. The internal viscosity of the red blood cell, calculated from the plasma viscosity and whole blood viscosity at 230 sec−1, decreases with increasing plasma fibrinogen concentration, in agreement with the direct measurements made of erythrocyte flexibility. It is proposed that at high shear rates an increase in plasma viscosity due to an elevation of fibrinogen concentration, is offset by a decrease in the rigidity of the erythrocytes, and these 2 effects counter-balance.

Relation of plasma fibrinogen concentration changes to human arteriosclerosis

1961 ◽  
Vol 16 (4) ◽  
pp. 660-664 ◽  
Author(s):  
L. O. Pilgeram
Keyword(s):  
Myocardial Infarction ◽  
Anticoagulant Therapy ◽  
Technical Assistance ◽  
Fibrinogen Level ◽  
Test System ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Inflammatory Conditions ◽  
Concentration Changes

A 35% increase (P < 0.001) in plasma fibrinogen levels was found in patients who recovered from the trauma of myocardial infarction and had not received anticoagulant therapy for 5 months preceding donation of plasma. The elevated fibrinogen levels are not a consequence of inflammatory conditions or traumatized tissue resulting from clinically overt vascular incidents. The fibrinogen level does not return to normal following a myocardial infarction. Occlusion of beta lipoproteins in the fibrin coagulum did not account for elevated levels of fibrinogen as shown by experiments where a) known quantities of alpha and beta lipoproteins were added to the test system, b) correction was made for the occlusion factor, and c) the increased yield due to this occlusion factor represented only a 3.5% increase in fibrinogen yield for as much as 300% increase in the level of beta lipoproteins. The fibrinogen level not only increases with age but also superimposes upon the arteriosclerotic an increase in addition to that occurring with increasing chronologic age. The role of fibrin in vascular hypertrophy and atheromatous development is emphasized. Possible causes for abnormal levels of fibrinogen are discussed. Note: (With the Technical Assistance of A. C. Schram and D. Mills) Submitted on December 5, 1960

Determination of Fibrinogen and Fibrinogenolytic Activity

1962 ◽  
Vol 08 (02) ◽  
pp. 297-310 ◽  
Author(s):  
Inga Marie Nilsson ◽  
Bertil Olow
Keyword(s):  
Thrombolytic Therapy ◽  
Fibrinolytic Activity ◽  
Fibrinogen Level ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Plasma Fibrinogen Level ◽  
Fibrinogenolytic Activity ◽  
The Difference ◽  
The Moment

SummaryA method for determining plasma fibrinogen and fibrinogenolytic activity, with epsilonaminocaproic acid (ε-ACA) as an inhibitor of fibrinolysis, in patients with high fibrinolytic activity, is described in detail.The fibrinogen was determined by a modification of Morrison’s syneresis method in parallel in 1. citrated plasma that was incubated for 2 hours at 37° C, after which further digestion was prevented by addition of ε-ACA and is referred to as fibrinogen A, and 2. in citrated plasma prepared from blood collected in tubes containing ε-ACA to prevent activation of plasminogen and is referred to as fibrinogen B. The difference between fibrinogen B and fibrinogen A gives the amount of lysed fibrinogen in 2 hours at 37° C. Fibrinogen B gives the fibrinogen concentration at the moment of sampling.The method is recommended to be used for evaluation of the true plasma fibrinogen level and the degree of fibrinogenolytic activity. This is particularly important during thrombolytic therapy.

scholarly journals Another Inherited Abnormality in Plasma Fibrinogen

1977 ◽  
Author(s):  
N. B. Bosch ◽  
C. L. Arocha-Piñango ◽  
A. Rodriguez ◽  
A. Ojeda ◽  
Z. Carvajal
Keyword(s):  
Ionic Strength ◽  
Electrophoretic Mobility ◽  
Factor Xiii ◽  
Polymerization Rate ◽  
Plasma Fibrinogen ◽  
Fibrinogen Concentration ◽  
Thrombin Time ◽  
Fibrin Polymerization ◽  
History Of ◽  
Α Chain

A prolonged thrombin and reptilase time with normal fibrinogen concentration in a 9 — years old girl, with no history of bleeding, prompted us to study the behaviour of her fibrinogen. Thrombin and reptilase time in different conditions, immunolectrophoresis, immunodiffusion and fibrin polymerization were performed. The thrombin time was partially -corrected by calcium, ionic strength and increasing concentration of thrombin. Fibrin polymerization rate and monomer aggregation time were moderately abnormal. Immunodiffusion and immunoelectrophoresis showed lines of identity with the normal. The electrophoretic -mobility of the α β and γ fibrin chains was normal, but there was a large amount of α — chain left in the cross-linked fibrin,in the presence of factor XIII. The above results suggest the presence of another fibrinogen variant.

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