Indole Alkaloid Formation and Storage in Cell Suspension Cultures of Catharanthus roseus

Planta Medica ◽  
1983 ◽  
Vol 48 (05) ◽  
pp. 20-23 ◽  
Author(s):  
D. Neumann ◽  
G. Krauss ◽  
M. Hieke ◽  
D. Gröger
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
And Storage

Medium-Induced Formation of Indole Alkaloids and Concomitant Changes of Interrelated Enzyme Activities in Cell Suspension Cultures of Catharanthus roseus

1981 ◽  
Vol 36 (1-2) ◽  
pp. 40-43 ◽  
Author(s):  
K.-H. Knobloch ◽  
B. Hansen ◽  
J. Berlin
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Regulatory Function ◽  
Tryptophan Decarboxylase ◽  
Induction Medium ◽  
Cell Transfer ◽  
Measuring Period

Abstract Recently medium conditions have been developed which stimulate the formation of the indole alkaloid ajmalicine in cell suspension cultures of Catharanthus roseus [6]. When cells were subjected to these conditions the alkaloid accumulation was preceded by a 12-fold increase of the specific activity of tryptophan decarboxylase. The enzyme activity showed a maximum two days after the cell transfer into the induction medium and subsequently declined. In contrast the activity of strictosidine synthase, the enzyme condensing tryptamine and secologanin, was present over the entire measuring period at a constant level. The intracellular content of tryp­tamine and ajmalicine increased during a period of 6 days after cell transfer and reached a plateau after that time. A possible regulatory function of tryptophan decarboxylase in indole alkaloid biosynthesis is discussed.

Cytokinins and ethylene stimulate indole alkaloid accumulation in cell suspension cultures of Catharanthus roseus by two distinct mechanisms

Plant Science ◽  
1998 ◽  
Vol 133 (1) ◽  
pp. 9-15 ◽  
Author(s):  
Abdelouahab Yahia ◽  
Claire Kevers ◽  
Thomas Gaspar ◽  
Jean-Claude Chénieux ◽  
Marc Rideau ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Catharanthus Roseus ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures
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