ChemInform Abstract: Enzymatic Formation of Raucaffricine, the Major Indole Alkaloid of Rauwolfia serpentina Cell-Suspension Cultures.

ChemInform ◽  
2010 ◽  
Vol 23 (10) ◽  
pp. no-no
Author(s):  
C. M. RUYTER ◽  
J. H. H. STOECKIGT
Keyword(s):  
Cell Suspension ◽  
Indole Alkaloid ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Rauwolfia Serpentina ◽  
Enzymatic Formation

Isolation and characterization of an UDPG-dependent glucosyltransferase activity from Rauwolfia serpentina Benth. cell suspension cultures

1994 ◽  
Vol 72 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Ralf Lutterbach ◽  
Carl Michael Ruyter ◽  
Joachim Stöckigt
Keyword(s):  
Enzyme Activity ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Rauwolfia Serpentina ◽  
Isolation And Characterization ◽  
Membrane Bound ◽  
Plant Families

From cell suspension cultures of Rauwolfia serpentina Benth. a new enzyme activity was isolated and its properties determined. The enzyme is a soluble protein and catalyzes the transfer of a glucose moiety from UDPG to a wide variety of phenolic compounds with p-nitrophenol as one of the best substrates (Km = 1.21 mM, UDPG = 0.54 mM). In contrast to the membrane-bound UDPG: vomilenine-21-OH-β-D-glucosyltransferase from Rauwolfia serpentina cells, this enzyme is not able to glucosylate indole alkaloids. The enzyme activity has been detected in 14 callus cultures belonging to 10 different plant families.

Novel Glucoalkaloids from Rauwolfia Cell Cultures – Acetylrauglucine and Related Glucosides

1988 ◽  
Vol 43 (7-8) ◽  
pp. 479-484 ◽  
Author(s):  
Carl Michael Ruyter ◽  
Helmut Schübel ◽  
Joachim Stöckigt
Keyword(s):  
Cell Suspension ◽  
Cell Cultures ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Production Medium ◽  
Rauwolfia Serpentina

From cell suspension cultures of Rauwolfia serpentina grown in an optimized production medium for the glucoalkaloid raucaffricine, a novel glucoalkaloid was isolated and identified as 17-O-acetyl-21-O-β-ᴅ-glucopyranosyl-ajmaline (acetylrauglucine). This alkaloid is formed in very small amounts (< 5 × 10-4%). The biogenetically related Nα-demethylated base (acetyl-nor-rauglucine) and the deacetyl product rauglucine have also been detected in culture extracts. In addition 21(.R)-(β-D-glucopyranosyl)-hydroxy-sarpagan-17-al has been isolated and identified as an artifact which originates from raucaffricine.

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