Qualitative Tests for Determination of Free Amino Groups on the Solid ­Support

2005 ◽  
pp. 1
Author(s):  
W. D. Lubell ◽  
J. W. Blankenship ◽  
G. Fridkin ◽  
R. Kaul
Keyword(s):  
Free Amino ◽  
Solid Support ◽  
Amino Groups

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

A simple colorimetric determination of the free amino groups in water soluble chitin derivatives using genipin

2010 ◽  
Vol 19 (3) ◽  
pp. 683-689 ◽  
Author(s):  
Luz M. Ramos-Ponce ◽  
Mireille Vega ◽  
Georgina C. Sandoval-Fabián ◽  
Edith Colunga-Urbina ◽  
Nagamani Balagurusamy ◽  
...  
Keyword(s):  
Free Amino ◽  
Water Soluble ◽  
Colorimetric Determination ◽  
Amino Groups ◽  
Chitin Derivatives

New Method for the Determination of Free Amino Groups in Intact Pure Proteins: Relationship to Available Lysine

1976 ◽  
Vol 59 (6) ◽  
pp. 1251-1254
Author(s):  
James M Purcell ◽  
Daniel J Quimby ◽  
James R Cavanaugh
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Free Amino ◽  
Amino Group ◽  
Amino Groups ◽  
Primary Amino ◽  
Available Lysine ◽  
Β Lactoglobulin ◽  
Group Concentration

Abstract A new rapid method for the quantitative and routine determination of free amino groups in intact pure proteins has been developed. Primary amino groups are labeled with fluorescamine and the labeled groups are detected by absorption spectroscopy in the range 375–390 nm. The amino group concentration can be determined in a few minutes without hydrolyzing the labeled protein and extracting a lysine derivative. The method was tested with the following proteins: lysozyme, α-lactalbumin, β-lactoglobulin, bovine serum albumin, ribonuclease, ribonuclease-S-peptide, and αsl-rasciii B. Application of this method to the estimation of available lysine is discussed.

scholarly journals The Free Amino Groups of the Proteus Flagella Protein. Determination of Dinitrophenyl Amino Acids Using Paper Chromatography.

1953 ◽  
Vol 7 ◽  
pp. 335-339 ◽  
Author(s):  
Claes Weibull ◽  
Artturi I. Virtanen ◽  
J. K. Miettinen ◽  
Artturi I. Virtanen ◽  
Nils Andreas Sörensen
Keyword(s):  
Amino Acids ◽  
Paper Chromatography ◽  
Free Amino ◽  
Amino Groups ◽  
Protein Determination

PREPARATION OF AN ESTRONE–PROTEIN CONJUGATE

1958 ◽  
Vol 36 (11) ◽  
pp. 1177-1184 ◽  
Author(s):  
L. Goodfriend ◽  
A. H. Sehon
Keyword(s):  
Serum Albumin ◽  
Free Amino ◽  
Electrophoretic Analysis ◽  
Amino Derivative ◽  
Rabbit Serum ◽  
Amino Groups ◽  
Protein Conjugate ◽  
Lysine Residues ◽  
Albumin Molecule

A procedure is described for the preparation of an estrone–protein conjugate. The 17-amino derivative of estrone was prepared and converted by phosgenation to estrone-17-isocyanate. The latter was coupled to rabbit serum albumin. Ultraviolet analysis and determination of free amino groups of both albumin and estrone–albumin conjugate showed that the latter contained about 25 estrone residues per albumin molecule. Electrophoretic analysis provided additional evidence that coupling had occurred at the ε-amino groups of the lysine residues of albumin.

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