Studies Concerning Starch-Complexed-Protein I. Spectrophotometric Determination of Free Amino Groups and Total Nitrogen

1967 ◽  
Vol 19 (6) ◽  
pp. 169-173 ◽  
Author(s):  
S. Rogols ◽  
J. E. Green
Keyword(s):  
Spectrophotometric Determination ◽  
Total Nitrogen ◽  
Free Amino ◽  
Amino Groups

Automated Determination of Free Amino Groups in Serum and Plasma Using 2,4,6-Trinitrobenzene Sulfonate

1969 ◽  
Vol 15 (9) ◽  
pp. 891-901 ◽  
Author(s):  
D W Palmer ◽  
T Peters
Keyword(s):  
Amino Acids ◽  
Free Amino Acids ◽  
Free Amino ◽  
Clinical Applications ◽  
Amino Groups ◽  
Automated Method ◽  
Total Free Amino Acids ◽  
Colored Complexes ◽  
Automated Determination

Abstract A simple automated method is described for determining the level of total free amino acids in the blood. The method utilizes the AutoAnalyzer, and is based on the formation of colored complexes by uniting free amino groups with 2,4,6-trinitrobenzene sulfonate (TNBS). Proteins do not interfere because the free amino acids are first separated by dialysis. Characteristics of the reaction and potential clinical applications of the procedure are discussed.

Spectrophotometric Determination of Amino-Groups

Nature ◽  
1953 ◽  
Vol 172 (4375) ◽  
pp. 459-460 ◽  
Author(s):  
DALLAS FRASER ◽  
H. G. HIGGINS
Keyword(s):  
Spectrophotometric Determination ◽  
Amino Groups

Studies on soil humic acids II: Observations on the estimation of free amino groups. Reactions of humic acid and lignin preparations with nitrous acid

1957 ◽  
Vol 48 (3) ◽  
pp. 352-360 ◽  
Author(s):  
J. M. Bremner
Keyword(s):  
Nitric Acid ◽  
Reaction Time ◽  
Humic Acid ◽  
Acid Hydrolysis ◽  
Sodium Hydroxide ◽  
Total Nitrogen ◽  
Nitrous Acid ◽  
Free Amino ◽  
Amino Nitrogen ◽  
Amino Groups

1. Free amino groups in humic acid preparations isolated from 0·5M-sodium hydroxide and 0·1M-sodium pyrophosphate (pH 7·0) extracts of various soils have been estimated by the nitrous acid method of Van Slyke (1929) and the fluorodinitrobenzene technique of Sanger (1945).2. The results obtained by the Van Slyke method using a reaction time of 15min. indicated that from 12 to 30% of the total nitrogen in the preparations examined was in the form of free amino groups. No free amino groups could be detected by the fluorodinitrobenzene technique.3. It is shown that lignin interferes with the estimation of amino groups by the Van Slyke method, and it is suggested that lignin or ligninderived material may be largely responsible for the high apparent amino-nitrogen values obtained with humic acid preparations by this method.4. The reaction of humic acid with nitrous acid resembles the reaction of lignin with nitrous acid in that it is accompanied by the fixation of nitrogen and the destruction of methoxyl groups. The reaction of lignin with nitrous acid is similar in many respects to its reaction with nitric acid.5. Only about one-third of the nitrogen fixed by lignin in its reaction with nitrous acid is removed by prolonged hydrolysis with 6N-HC1, and most of the nitrogen so released is in the form of ammonia. A small amount of the nitrogen liberated by acid hydrolysis is in the form of hydroxylamine.

A simple colorimetric determination of the free amino groups in water soluble chitin derivatives using genipin

2010 ◽  
Vol 19 (3) ◽  
pp. 683-689 ◽  
Author(s):  
Luz M. Ramos-Ponce ◽  
Mireille Vega ◽  
Georgina C. Sandoval-Fabián ◽  
Edith Colunga-Urbina ◽  
Nagamani Balagurusamy ◽  
...  
Keyword(s):  
Free Amino ◽  
Water Soluble ◽  
Colorimetric Determination ◽  
Amino Groups ◽  
Chitin Derivatives

New Method for the Determination of Free Amino Groups in Intact Pure Proteins: Relationship to Available Lysine

1976 ◽  
Vol 59 (6) ◽  
pp. 1251-1254
Author(s):  
James M Purcell ◽  
Daniel J Quimby ◽  
James R Cavanaugh
Keyword(s):  
Bovine Serum Albumin ◽  
Bovine Serum ◽  
Free Amino ◽  
Amino Group ◽  
Amino Groups ◽  
Primary Amino ◽  
Available Lysine ◽  
Β Lactoglobulin ◽  
Group Concentration

Abstract A new rapid method for the quantitative and routine determination of free amino groups in intact pure proteins has been developed. Primary amino groups are labeled with fluorescamine and the labeled groups are detected by absorption spectroscopy in the range 375–390 nm. The amino group concentration can be determined in a few minutes without hydrolyzing the labeled protein and extracting a lysine derivative. The method was tested with the following proteins: lysozyme, α-lactalbumin, β-lactoglobulin, bovine serum albumin, ribonuclease, ribonuclease-S-peptide, and αsl-rasciii B. Application of this method to the estimation of available lysine is discussed.

Spectrophotometric determination of browning products of glycation of protein amino groups based on their reactivity with nitro blue tetrazolium salts

The Analyst ◽  
1988 ◽  
Vol 113 (7) ◽  
pp. 1101 ◽  
Author(s):  
Gian Marco Ghiggeri ◽  
Giovanni Candiano ◽  
Fabrizio Ginevri ◽  
Roberta Oleggini ◽  
Maria Teresa Piccardo ◽  
...  
Keyword(s):  
Spectrophotometric Determination ◽  
Nitro Blue Tetrazolium ◽  
Amino Groups ◽  
Tetrazolium Salts ◽  
Blue Tetrazolium
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