In vitro enhancement of extracellular matrix formation as natural bioscaffold for stem cell culture

2017 ◽  
Author(s):  
Aroem Naroeni ◽  
Qonitha Shalihah ◽  
Sofy Meilany
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Stem Cell Culture

scholarly journals Stem cell culture on polyvinyl alcohol hydrogels having different elasticity and immobilized with ECM-derived oligopeptides

2017 ◽  
Vol 37 (7) ◽  
pp. 647-660 ◽  
Author(s):  
Saradaprasan Muduli ◽  
Li-Hua Chen ◽  
Meng-Pei Li ◽  
Zhao-wen Heish ◽  
Cheng-Hui Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Cell Fate ◽  
Es Cells ◽  
Embryonic Stem ◽  
Poly Vinyl Alcohol ◽  
Hematopoietic Stem ◽  
Stem Cell Culture

Abstract The physical characteristics of cell culture materials, such as their elasticity, affect stem cell fate with respect to cell proliferation and differentiation. We systematically investigated the morphologies and characteristics of several stem cell types, including human amniotic-derived stem cells, human hematopoietic stem cells, human induced pluripotent stem (iPS) cells, and embryonic stem (ES) cells on poly(vinyl alcohol) (PVA) hydrogels immobilized with and without extracellular matrix-derived oligopeptide. Human ES cells did not adhere well to soft PVA hydrogels immobilized with oligovitronectin, whereas they did adhere well to PVA hydrogel dishes with elasticities greater than 15 kPa. These results indicate that biomaterials such as PVA hydrogels should be designed to possess minimum elasticity to facilitate human ES cell attachment. PVA hydrogels immobilized with and without extracellular matrix-derived oligopeptides are excellent candidates of cell culture biomaterials for investigations into how cell culture biomaterial elasticity affects stem cell culture and differentiation.

Electrospinning adipose tissue-derived extracellular matrix for adipose stem cell culture

2012 ◽  
Vol 100A (7) ◽  
pp. 1716-1724 ◽  
Author(s):  
Michael P. Francis ◽  
Patrick C. Sachs ◽  
Parthasarathy A. Madurantakam ◽  
Scott A. Sell ◽  
Lynne W. Elmore ◽  
...  
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Adipose Tissue ◽  
Stem Cell ◽  
Adipose Stem Cell ◽  
Stem Cell Culture

Stem cell culture: mimicking the stem cell niche in vitro

2013 ◽  
pp. 33-68
Author(s):  
Tiago G. Fernandes ◽  
Maria Margarida Diogo ◽  
Joaquim M.S. Cabral
Keyword(s):  
Cell Culture ◽  
Stem Cell ◽  
Stem Cell Niche ◽  
Stem Cell Culture ◽  
Cell Niche

Extracellular Matrix Isolated From Foreskin Fibroblasts Supports Long-Term Xeno-Free Human Embryonic Stem Cell Culture

2010 ◽  
Vol 19 (4) ◽  
pp. 547-556 ◽  
Author(s):  
Guoliang Meng ◽  
Shiying Liu ◽  
Xiangyun Li ◽  
Roman Krawetz ◽  
Derrick E. Rancourt
Keyword(s):  
Cell Culture ◽  
Extracellular Matrix ◽  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Stem Cell Culture

scholarly journals Optimization of mouse embryonic stem cell culture for organoid and chimeric mice production

2020 ◽  
Author(s):  
Cécilie Martin-Lemaitre ◽  
Yara Alcheikh ◽  
Ronald Naumann ◽  
Alf Honigmann
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Stem Cell ◽  
Embryonic Stem Cells ◽  
Suspension Culture ◽  
Cell Biology ◽  
Embryonic Stem ◽  
Model Systems ◽  
Stem Cell Culture

SummaryIn vitro stem cell culture is demanding in terms of manpower and media supplements. In recent years, new protocols have been developed to expand pluripotent embryonic stem cells in suspension culture, which greatly simplifies cell handling and scalability. However, it is still unclear how suspension culture protocols with different supplements affect pluripotency, cell homogeneity and cell differentiation compared to established adherent culture methods. Here we tested four different culture conditions for mouse embryonic stem cells (mESC) and quantified chimerism and germ line transmission as well as in vitro differentiation into three-dimensional neuro-epithelia. We found that suspension culture supplemented with CHIR99021/LIF offers the best compromise between culturing effort, robust pluripotency and cell homogeneity. Our work provides a guideline for simplifying mESC culture and should encourage more cell biology labs to use stem cell-based organoids as model systems.

Sign in / Sign up

Export Citation Format

Share Document