scholarly journals Preparation of Rolled Epidermis of Vicia Faba L. So That Stomata are the Only Viable Cells: Analysis of Guard Cell Potassium by Flame Photometry

1973 ◽  
Vol 26 (2) ◽  
pp. 309 ◽  
Author(s):  
WG Allaway ◽  
TC Hsiao

A rolling technique is described with which cells in epidermal strips of V. faba were differentially broken so that only guard cells remained alive and functional. In rolled epidermis potassium was retained only in live guard cells, as judged by staining with cobaltinitrite.

Planta ◽  
1990 ◽  
Vol 181 (3) ◽  
Author(s):  
H.M. Brindley
Keyword(s):  

2017 ◽  
Vol 37 (17) ◽  
Author(s):  
周健 ZHOU Jian ◽  
王亚男 WANG Ya'nan ◽  
马丹炜 MA Danwei ◽  
黄素 HUANG Su ◽  
辛文媛 XIN Wenyuan ◽  
...  

2010 ◽  
Vol 61 (13) ◽  
pp. 3749-3758 ◽  
Author(s):  
B. Li ◽  
G. Liu ◽  
Y. Deng ◽  
M. Xie ◽  
Z. Feng ◽  
...  

Planta ◽  
1978 ◽  
Vol 142 (1) ◽  
pp. 61-65 ◽  
Author(s):  
Teruo Ogawa ◽  
Hirohisa Ishikawa ◽  
Keizo Shimada ◽  
Kazuo Shibata

1985 ◽  
Vol 78 (1) ◽  
pp. 51-56 ◽  
Author(s):  
David A. Grantz ◽  
Tuan-Hua David Ho ◽  
Scott J. Uknes ◽  
John M. Cheeseman ◽  
John S. Boyer

1972 ◽  
Vol 50 (6) ◽  
pp. 1405-1413 ◽  
Author(s):  
W. G. Allaway ◽  
George Setterfield

Stomata of Vicia faba and Allium porrum were examined in thin section with the electron microscope. Guard cells contained numerous mitochondria, few plastids, and relatively small vacuoles traversed by many strands of cytoplasm. Spherosomes were often observed but were variable in occurrence. Endoplasmic reticulum and dictyosomes were present, although not well developed. Scattered microtubules were present at the periphery of the cells. Microbodies were very rarely observed in guard cells and no plasmodesmata were ever seen in the guard cell walls. Plastids were small and irregular in outline in guard cells of both species. Guard cell plastids of V. faba contained abundant large starch granules. In both species thylakoids were few and grana were small in comparison with mesophyll plastids. The inner of the two bounding membranes of guard cell chloroplasts was extensively invaginated, forming a peripheral reticulum. This was not observed in mesophyll plastids of these species. Small groups of microtubule-like structures were often observed in V. faba guard cell plastids; microtubule-like structures were less frequent in A. porrum plastids, and were not in groups. The structures described are compared with those of other epidermal cells and mesophyll cells, and are discussed in relation to guard cell physiology.


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