Fluorescence Microscopy Study of Protein Adsorption at Modified Glassy Carbon Surfaces

Glassy carbon (GC) surfaces were modified with thin films by means of electrochemically assisted reduction of aryl diazonium salts and oxidation of primary amines. GC plates with modified and unmodified areas were exposed to solutions of fluorescently labelled bovine serum albumin (BSA-FITC). Fluorescence microscopy was found to be a simple and direct method for comparing adsorption of BSA-FITC between the different areas. Modification with methylbenzene, hexylbenzene, poly(ethylene glycol) benzene, and tetraethylene glycol diamine groups increases protein adsorption relative to unmodified GC. Hexylamine and the poly(ethylene glycol) diamine ED-2003 reduce protein adsorption. The results give insight into some factors controlling protein adsorption at these surface films.

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Measurements of Interbilayer Forces and Protein Adsorption on Uncharged Lipid Bilayers Displaying Poly(ethylene glycol) Chains†

Biochemistry ◽

10.1021/bi992095r ◽

2000 ◽

Vol 39 (12) ◽

pp. 3441-3451 ◽

Cited By ~ 69

Author(s):

Nadezhda V. Efremova ◽

Bruce Bondurant ◽

David F. O'Brie ◽

Deborah E. Leckband

Keyword(s):

Ethylene Glycol ◽

Protein Adsorption ◽

Lipid Bilayers ◽

Poly Ethylene Glycol ◽

Poly Ethylene

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Poly(ethylene glycol) Grafting to Poly(ether imide) Membranes: Influence on Protein Adsorption and Thrombocyte Adhesion

Macromolecular Bioscience ◽

10.1002/mabi.201300309 ◽

2013 ◽

Vol 13 (12) ◽

pp. 1720-1729 ◽

Cited By ~ 25

Author(s):

Axel T. Neffe ◽

Maik von Ruesten-Lange ◽

Steffen Braune ◽

Karola Luetzow ◽

Toralf Roch ◽

...

Keyword(s):

Ethylene Glycol ◽

Protein Adsorption ◽

Poly Ethylene Glycol ◽

Poly Ethylene

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Application of MS-Based Proteomics to Study Serum Protein Adsorption/Absorption and Complement C3 Activation on Poly(ethylene glycol) Hydrogels

Journal of Biomaterials Science Polymer Edition ◽

10.1163/092050610x508400 ◽

2011 ◽

Vol 22 (10) ◽

pp. 1343-1362 ◽

Cited By ~ 14

Author(s):

Xintong Wang ◽

David R. Schmidt ◽

Evan J. Joyce ◽

W. John Kao

Keyword(s):

Ethylene Glycol ◽

Protein Adsorption ◽

Serum Protein ◽

Complement C3 ◽

Poly Ethylene Glycol ◽

Poly Ethylene

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Quantitative Characterization of the Flexibility of Poly(ethylene glycol) Chains Attached to a Glassy Carbon Electrode

Macromolecules ◽

10.1021/ma990560m ◽

1999 ◽

Vol 32 (18) ◽

pp. 5829-5835 ◽

Cited By ~ 18

Author(s):

Agnès Anne ◽

Jacques Moiroux

Keyword(s):

Ethylene Glycol ◽

Glassy Carbon Electrode ◽

Glassy Carbon ◽

Carbon Electrode ◽

Poly Ethylene Glycol ◽

Quantitative Characterization ◽

Poly Ethylene

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Synthesis of Mono- and Dithiols of Tetraethylene Glycol and Poly(ethylene glycol)s via Enzyme Catalysis

Catalysts ◽

10.3390/catal9030228 ◽

2019 ◽

Vol 9 (3) ◽

pp. 228 ◽

Cited By ~ 4

Author(s):

Prajakatta Mulay ◽

Gayatri Shrikhande ◽

Judit Puskas

Keyword(s):

Nmr Spectroscopy ◽

Ethylene Glycol ◽

1H Nmr ◽

Enzyme Catalysis ◽

1H Nmr Spectroscopy ◽

13C Nmr Spectroscopy ◽

Tetraethylene Glycol ◽

Candida Antarctica Lipase ◽

Poly Ethylene Glycol ◽

Poly Ethylene

This paper investigates the transesterification of methyl 3-mercaptopropionate (MP-SH) with tetraethylene glycol (TEG) and poly(ethylene glycol)s (PEG)s catalyzed by Candida antarctica Lipase B (CALB) without the use of solvent (in bulk). The progress of the reactions was monitored by 1H-NMR spectroscopy. We found that the reactions proceeded in a step-wise manner, first producing monothiols. TEG-monothiol was obtained in 15 min, while conversion to dithiol took 8 h. Monothiols from PEGs with Mn = 1000 and 2050 g/mol were obtained in 8 and 16 h, respectively. MALDI-ToF mass spectrometry verified the absence of dithiols. The synthesis of dithiols required additional fresh CALB and MP-SH. The structure of the products was confirmed by 1H-NMR and 13C-NMR spectroscopy. Enzyme catalysis was found to be a powerful tool to effectively synthesize thiol-functionalized TEGs and PEGs.

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