Convergent Solution Phase Synthesis of Chimeric Oligonucleotides by a 2+2 and 3+3 Phosphoramidite Strategy

2010 ◽  
Vol 63 (2) ◽  
pp. 227 ◽  
Author(s):  
Chih-Hau Chen ◽  
Wei-Yu Chen ◽  
Yu-Chie Chen ◽  
Ming-Juan Lee ◽  
Chyuan-Der Huang ◽  
...  

A chimeric oligonucleotide tetramer and hexamer were synthesized by the phosphoramidite approach using a 2+2 and 3+3 strategy, respectively. The concept of convergent synthesis provides an efficient route toward the synthesis of longer chimeric oligonucleotides, such as small interfering RNA oligonucleotides without the pollution of n – 1 or shorter failures. This methodology offers an efficient and economical way to scale-up the synthesis of high purity oligonucleotides for clinical trials and commercial uses.

ChemInform ◽  
2010 ◽  
Vol 41 (23) ◽  
pp. no-no
Author(s):  
Chih-Hau Chen ◽  
Wei-Yu Chen ◽  
Yu-Chie Chen ◽  
Ming-Juan Lee ◽  
Chyuan-Der Huang ◽  
...  

2003 ◽  
Vol 68 (11) ◽  
pp. 4464-4471 ◽  
Author(s):  
Robert C. Reid ◽  
Giovanni Abbenante ◽  
Stephen M. Taylor ◽  
David P. Fairlie

Author(s):  
François Morvan ◽  
Aude-Emmanuelle Navarro ◽  
Cécile Dueymes ◽  
Ilaria Adamo ◽  
Andreas Schoenberger ◽  
...  

ChemInform ◽  
2010 ◽  
Vol 33 (35) ◽  
pp. 123-123
Author(s):  
Young K. Yun ◽  
John A. Porco Jr. ◽  
Jeff Labadie

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