Convergent Solution Phase Synthesis of Chimeric Oligonucleotides by a 2+2 and 3+3 Phosphoramidite Strategy
Keyword(s):
Scale Up
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A chimeric oligonucleotide tetramer and hexamer were synthesized by the phosphoramidite approach using a 2+2 and 3+3 strategy, respectively. The concept of convergent synthesis provides an efficient route toward the synthesis of longer chimeric oligonucleotides, such as small interfering RNA oligonucleotides without the pollution of n – 1 or shorter failures. This methodology offers an efficient and economical way to scale-up the synthesis of high purity oligonucleotides for clinical trials and commercial uses.
2003 ◽
Vol 68
(11)
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pp. 4464-4471
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Keyword(s):
1999 ◽
Vol 9
(4)
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pp. 547-550
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Keyword(s):
2005 ◽
Keyword(s):
2003 ◽
Vol 62
(2)
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pp. 78-87
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1995 ◽
Vol 5
(1)
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pp. 39-47
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