scholarly journals A Theoretical Assessment of the Structure Determination of Multi-Span Membrane Proteins by Oriented Sample Solid-State NMR Spectroscopy

2020 ◽  
Vol 73 (3) ◽  
pp. 246 ◽  
Author(s):  
Daniel K. Weber ◽  
Gianluigi Veglia
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Solid State Nmr ◽  
Direct Determination ◽  
Oriented Sample ◽  
Dynamics Simulations ◽  
G Protein Coupled ◽  
Theoretical Assessment

Oriented sample solid-state NMR (OS-ssNMR) spectroscopy allows the direct determination of the structure and topology of membrane proteins reconstituted into aligned lipid bilayers. Although OS-ssNMR theoretically has no upper size limit, its application to multi-span membrane proteins has not been established because most studies have been restricted to single- or dual-span proteins and peptides. Here, we present a critical assessment of the application of this method to multi-span membrane proteins. We used molecular dynamics simulations to back-calculate [15N-1H] separated local field (SLF) spectra from a G protein-coupled receptor (GPCR) and show that fully resolved spectra can be obtained theoretically for a multi-span membrane protein with currently achievable resonance linewidths.

scholarly journals Direct Determination of a Molecular Torsional Angle in the Membrane Protein Rhodopsin by Solid-State NMR

1997 ◽  
Vol 119 (29) ◽  
pp. 6853-6857 ◽  
Author(s):  
X. Feng ◽  
P. J. E. Verdegem ◽  
Y. K. Lee ◽  
D. Sandström ◽  
M. Edén ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Protein ◽  
Solid State Nmr ◽  
Direct Determination ◽  
Torsional Angle

scholarly journals Towards a native environment: structure and function of membrane proteins in lipid bilayers by NMR

2021 ◽  
Author(s):  
Kai Xue ◽  
Kumar Tekwani Movellan ◽  
Xizhou Cecily Zhang ◽  
Eszter E. Najbauer ◽  
Marcel C. Forster ◽  
...  
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Small Molecules ◽  
Lipid Bilayers ◽  
Biological Samples ◽  
Solid State Nmr ◽  
Structure And Function ◽  
Versatile Technique ◽  
And Function

Solid-state NMR (ssNMR) is a versatile technique that can be used for the characterization of various materials, ranging from small molecules to biological samples, including membrane proteins, as reviewed here.

Solid-State NMR of Membrane Proteins in Lipid Bilayers: To Spin or Not To Spin?

2021 ◽  
Author(s):  
Tata Gopinath ◽  
Daniel Weber ◽  
Songlin Wang ◽  
Erik Larsen ◽  
Gianluigi Veglia
Keyword(s):  
Solid State ◽  
Membrane Proteins ◽  
Lipid Bilayers ◽  
Solid State Nmr

Direct determination of a molecular torsional angle by solid-state NMR

1996 ◽  
Vol 257 (3-4) ◽  
pp. 314-320 ◽  
Author(s):  
X. Feng ◽  
Y.K. Lee ◽  
D. Sandstrom ◽  
M. Edén ◽  
H. Maisel ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Direct Determination ◽  
Torsional Angle

Direct Determination of a Peptide Torsional Angle ψ by Double-Quantum Solid-State NMR

1997 ◽  
Vol 119 (49) ◽  
pp. 12006-12007 ◽  
Author(s):  
X. Feng ◽  
M. Edén ◽  
A. Brinkmann ◽  
H. Luthman ◽  
L. Eriksson ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Nmr ◽  
Direct Determination ◽  
Double Quantum ◽  
Torsional Angle ◽  
Quantum Solid
Sign in / Sign up

Export Citation Format

Share Document