Anther Culture of Australian Wheat Germplasm Using Modified C17 Medium and Membrane Rafts

1991 ◽  
Vol 18 (4) ◽  
pp. 357 ◽  
Author(s):  
DJ Luckett ◽  
S Venkatanagappa ◽  
NL Darvey ◽  
RA Smithard
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Wheat Breeding ◽  
Membrane Rafts ◽  
Wheat Germplasm ◽  
Haploid Production ◽  
Australian Wheat ◽  
Culture Procedure

Experiments with wheat anther culture were conducted with the aim of assembling a workable system for doubled-haploid production for Australian wheat breeding. One Australian cultivar, Grebe, carrying the 1BL/1RS translocation was particularly responsive. A modified C17 medium was developed which proved effective when solidified with agarose or when used as a liquid in conjunction with membrane support rafts. The membrane rafts have great potential to assist the development of the anther culture procedure since the medium can be rapidly replenished or replaced with minimal disturbance of the tissue.

A cost-based index of anther culture response in diverse wheat-breeding germplasm

1995 ◽  
Vol 35 (3) ◽  
pp. 395 ◽  
Author(s):  
DC Ling ◽  
DJ Luckett ◽  
NL Darvey ◽  
XL Ding
Keyword(s):  
Anther Culture ◽  
Low Dose ◽  
Wheat Breeding ◽  
Significant Loss ◽  
Diverse Range ◽  
Persistent Problem ◽  
Irradiation Treatment ◽  
Australian Wheat ◽  
Laboratory Procedures ◽  
Anther Culture Response

Anther culture response in a diverse range of Australian wheat-breeding genotypes was tested and expressed as a 'culturability index' based on the actual costs of the various laboratory procedures. When the delay from harvest to plating was up to 12 h, the use of low dose gamma irradiation did not increase the anther culture response in this array of genotypes. This is in contrast to previous findings for which spike harvesting, irradiation, and plating was completed within 3 h and the irradiation treatment increased the anther culture response. Internal contamination of immature spike explants with Fusarium poae caused significant loss of cultures and may be a persistent problem for cereal anther culture. For the genotypes producing green regenerants, costs ranged from $A1.40 to $85/green regenerant.

scholarly journals DOUBLED HAPLOID PRODUCTION IN CAPSICUM ANNUUM L. USING ANTHER CULTURE : A REVIEW

2021 ◽  
Vol 21 (Suppliment-1) ◽  
pp. 168-173
Author(s):  
Kaushik Jha ◽  
Pradeep Kumar Choudhary ◽  
Arun Agarwal
Keyword(s):  
Anther Culture ◽  
Capsicum Annuum ◽  
Doubled Haploid ◽  
Capsicum Annuum L ◽  
Haploid Production

Embryogenesis and doubled haploid production from anther culture in gentian (Gentiana triflora)

2010 ◽  
Vol 102 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Hisako Doi ◽  
Ryo Takahashi ◽  
Takashi Hikage ◽  
Yoshihito Takahata
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Gentiana Triflora ◽  
Haploid Production

Doubled haploid production via anther culture in annual, winter type of caraway (Carum carvi L.)

2008 ◽  
Vol 31 (1) ◽  
Author(s):  
Iva Smýkalová ◽  
Prokop Šmirous ◽  
Michaela Kubošiová ◽  
Nikol Gasmanová ◽  
Miroslav Griga
Keyword(s):  
Anther Culture ◽  
Doubled Haploid ◽  
Winter Type ◽  
Carum Carvi ◽  
Haploid Production

Bread Wheat Doubled Haploid Production by Anther Culture

2021 ◽  
pp. 227-244
Author(s):  
Ana María Castillo ◽  
Isabel Valero-Rubira ◽  
Sandra Allué ◽  
María Asunción Costar ◽  
María Pilar Vallés
Keyword(s):  
Anther Culture ◽  
Bread Wheat ◽  
Doubled Haploid ◽  
Haploid Production

scholarly journals In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

2021 ◽  
Vol 58 (1) ◽  
pp. 31-45
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Janos Pauk
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Chromosome Doubling ◽  
Culture Media ◽  
Triticum Aestivum L ◽  
Technology Application ◽  
Breeding Material ◽  
Haploid Production

In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

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