Effects of fertilisation on microbial communities in short-term coal mine reclamation

Soil Research ◽  
2020 ◽  
Vol 58 (8) ◽  
pp. 779
Author(s):  
Jian Zhang ◽  
Yinghe Xie ◽  
Ying Wei ◽  
Huisheng Meng ◽  
Yanzhuan Cao ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Soil Properties ◽  
Coal Mining ◽  
Coal Mine ◽  
Bacterial Communities ◽  
Community Diversity ◽  
Short Term ◽  
Beneficial Effects

The recovery of the belowground microbial community structure and diversity that occurs in long-term coal mining reclamation is critical to reclamation success. However, long-term coal mining reclamation can take ~10–30 years. Therefore, finding an effective method for promoting coal mine soil restoration in the short-term is necessary to minimise reclamation time. This study investigated the response of soil bacterial communities to fertilisation along a chronosequence of short-term reclamation. Fertilised and unfertilised soils with three short-term reclamation stages were examined to characterise soil properties, as well as bacterial structure and diversity. Fertilisation promoted available nitrogen, phosphate, potassium, and soil organic matter, as well as benefits in bacterial community diversity across the three stages, with the most beneficial effects at 7 years. 16S rRNA sequencing data showed that the predominant phyla across all soils were Proteobacteria, Actinobacteria, Acidobacteria, Bacteroidetes, Chloroflexi, and Gemmatimonadetes. Abundance of each phylum was altered by reclamation time and fertilisation. Clustering and functional analysis indicated that the bacterial community structure in soils with a longer reclamation time was more similar to that in natural soils, suggesting that longer reclamation resulted in increased soil activity and bacterial community diversity, which is likely also true for fertilisation. Our results demonstrate that reclamation duration is the main driving force to recover soil properties and bacterial communities, and fertilisation could enhance the beneficial effects with longer reclamation duration. Therefore, short-term reclamation, combined with fertiliser, is a potential strategy to improve soil conditions in coal mine areas and shorten the recovery time of reclaimed soils.

Effects of Long-Term Elevated CO2 on Rhizosphere and Bulk Soil Bacterial Community Structure in Pinus sylvestriformis Seedlings Fields

2011 ◽  
Vol 343-344 ◽  
pp. 351-356
Author(s):  
Xia Jia ◽  
Chun Juan Zhou
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Pinus Sylvestriformis ◽  
Soil Bacterial Community Structure ◽  
Gradient Gel Electrophoresis ◽  
Polymerase Chain

The effect of long-term elevated CO2(as open top chambers) on rhizosphere and bulk bacterial community structure in Pinus sylvestriformis seedlings field was investigated in July, August, and September. The bacterial communities were processed by Denaturing Gradient Gel Electrophoresis (DGGE) analysis of bacterial 16S rDNA fragments amplified by PCR (Polymerase Chain Reaction) from DNA extracted directly from soil. DGGE profiles from rhizosphere samples showed large changes in rhizosphere bacterial community under elevated CO2compared to ambient except for that in September. For bulk samples, bacterial community structure changed when exposed to elevated CO2in three months. With the exception of bulk samples in August, a similitude of bacterial communities structures existed between different elevated CO2concentrations by analyzing UPGMA dendrogram based on Jaccard’s coefficient.

scholarly journals Silicon application and related changes in soil bacterial community dynamics reduced ginseng black spot incidence in Panax ginseng in a short-term study

2019 ◽  
Author(s):  
Meijia Li ◽  
Qiuxia Wang ◽  
Zhengbo Liu ◽  
Xiaoxi Pan ◽  
Yayu Zhang
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Panax Ginseng ◽  
Bacterial Communities ◽  
Community Dynamics ◽  
Bacterial Flora ◽  
Black Spot ◽  
Rrna Gene ◽  
Short Term ◽  
Physical And Chemical

Abstract Background This study analyzed the effect of silicon (Si) application on the occurrence of ginseng black spot caused by Alternaria panax . We explored the differences in soil physical and chemical factors and microbial community structure following Si application as well as the key factors that affected the occurrence of ginseng black spot in soil. Potted Panax ginseng plants were used to assess the effect of Si treatment on ginseng black spot. Soil physical and chemical properties were comprehensively analyzed. Bacterial communities were analyzed using Illumina HiSeq sequencing targeting the 16S rRNA gene. Results After inoculation with A. panax , the morbidity (and morbidity index) of ginseng with and without Si was 52% (46) and 83% (77), respectively. Soil physical and chemical analysis showed that under the ginseng black spot inoculation, bacterial communities were mainly affected by pH and available potassium, followed by ammonium nitrogen and available Si. NMDS and PLS-DA analyses and the heat maps of relative abundance revealed that Si application elevated the resistance of ginseng black spot as regulated by the abundance and diversity of bacterial flora in rhizosphere soils. Heatmap analysis at the genus level revealed that A. panax + Si inoculations significantly increased the soil community abundance of Sandaracinus , Polycyclovorans , Hirschia , Haliangium , Nitrospira , Saccharothrix , Aeromicrobium , Luteimonas , and Rubellimicrobium and led to a bacterial community structure with relative abundances that were significantly similar to that of untreated soil. Conclusions Short-term Si application also significantly regulated the structural impact on soil microorganisms caused by ginseng black spot. Our findings indicated that Si applications may possibly be used in the prevention and treatment of ginseng black spot.

scholarly journals Effect of straw biochar amendment on tobacco growth, soil properties, and rhizosphere bacterial communities

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jiayu Zheng ◽  
Jixu Zhang ◽  
Lin Gao ◽  
Rui Wang ◽  
Jiaming Gao ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Soil Properties ◽  
Bacterial Communities ◽  
Agronomic Traits ◽  
Diversity Indices ◽  
Community Diversity ◽  
High Dose ◽  
Rhizosphere Soils ◽  
Tobacco Growth ◽  
Biochar Amendment

AbstractBiochar is an effective soil conditioner. However, we have limited understanding of biochar effects on the tobacco growth and bacterial communities in rhizosphere. The aim of this study was to investigate the effects of different straw biochar amendment (0, 2, 10, and 50 g/kg dry soil) on tobacco growth, soil properties, and bacterial communities in rhizosphere by pot trials. Most of tobacco agronomic traits increased when the application rate varied from 0 to 10 g/kg, but were inhibited by 50 g/kg of biochar application. Soil pH, SOC, available nutrients and soil urease, invertase, and acid phosphatase activities were all increased with the biochar application, whereas catalase activity decreased or remained unchanged. The OTUs and bacterial community diversity indices differed with the biochar application doses in rhizosphere and non-rhizosphere soils. And significant differences in bacterial communities were found between the rhizosphere and non-rhizosphere soils despite the biochar addition. Firmicutes, Proteobacteria, Acidobacteria, Bacteroidetes, and Actinobacteria were the dominant phyla in all soil samples, but they had different abundances in different treatment influenced by the rhizosphere and biochar effect. The high dose of biochar (50 g/kg) decreased the similarity of soil bacterial community structure in rhizosphere compared with those in non-rhizosphere soil. These results provide a better understanding of the microecological benefits of straw biochar in tobacco ecosystem.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Impact of long-term industrial contamination on the bacterial communities in urban river sediments

2020 ◽  
Author(s):  
Lei Zhang ◽  
Demei Tu ◽  
Xingchen Li ◽  
Wenxuan Lu ◽  
Jing Li
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Industrial Pollution ◽  
River Sediments ◽  
Urban River ◽  
Industrial Contamination ◽  
Different Types

Abstract Background: The contamination of the aquatic environment of urban rivers with industrial wastewater has affected the abiotic conditions and biological activities of the trophic levels of the ecosystem, particularly sediments. However, most current research about microorganism in urban aquatic environments has focused on indicator bacteria related to feces and organic pollution. Meanwhile, they ignored the interactions among microorganisms. To deeply understand the impact of industrial contamination on microbial community, we study the bacterial community structure and diversity in river sediments under the influence of different types of industrial pollution by Illumina MiSeq high-throughput sequencing technology and conduct a more detailed analysis of microbial community structure through co-occurrence networks.Results: The overall community composition and abundance of individual bacterial groups differed between samples. In addition, redundancy analysis indicated that the structure of the bacterial community in river sediments was influenced by a variety of environmental factors. TN, TP, TOC and metals (Cu, Zn and Cd) were the most important driving factors that determined the bacterial community in urban river sediments (P <0.01). According to PICRUSt analysis, the bacterial communities in different locations had similar overall functional profiles. It is worth noting that the 15 functional genes related to xenobiotics biodegradation and metabolism were the most abundant in the same location. The non-random assembly patterns of bacterial composition in different types of industrially polluted sediments were determined by a co-occurrence network. Environmental conditions resulting from different industrial pollutants may play an important role in determining their co-occurrence patterns of these bacterial taxa. Among them, the bacterial taxa involved in carbon and nitrogen cycles in module I were relatively abundant, and the bacterial taxa in module II were involved in the repair of metal pollution.Conclusions: Our data indicate that long-term potential interactions between different types of industrial pollution and taxa collectively affect the structure of the bacterial community in urban river sediments.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Tao Zhang ◽  
Zhongke Wang ◽  
Xinhua Lv ◽  
Hanli Dang ◽  
Li Zhuang
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Abstract Ferula sinkiangensis (F. sinkiangensis) is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, F. sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of F. sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and changed trend of F. sinkiangensis rhizosphere bacterial community diversity and abundance on slope position and soil depth and found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in F. sinkiangensis rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in F. sinkiangensis. In addition, Soil physicochemical factors jointly explained 24.28% of variation in F. sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of F. sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

scholarly journals Bacterial Communities in the Rhizosphere and Phyllosphere of Halophytes and Drought-Tolerant Plants in Mediterranean Ecosystems

2020 ◽  
Vol 8 (11) ◽  
pp. 1708
Author(s):  
Savvas Genitsaris ◽  
Natassa Stefanidou ◽  
Kleopatra Leontidou ◽  
Theodora Matsi ◽  
Katerina Karamanoli ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Soil Properties ◽  
Bacterial Communities ◽  
Mediterranean Ecosystems ◽  
Community Diversity ◽  
Sampling Location ◽  
Rrna Gene ◽  
Sodic Soils ◽  
Drought Tolerant ◽  
Tolerant Plants

The aim of the study was to investigate the bacterial community diversity and structure by means of 16S rRNA gene high-throughput amplicon sequencing, in the rhizosphere and phyllosphere of halophytes and drought-tolerant plants in Mediterranean ecosystems with different soil properties. The locations of the sampled plants included alkaline, saline-sodic soils, acidic soils, and the volcanic soils of Santorini Island, differing in soil fertility. Our results showed high bacterial richness overall with Proteobacteria and Actinobacteria dominating in terms of OTUs number and indicated that variable bacterial communities differed depending on the plant’s compartment (rhizosphere and phyllosphere), the soil properties and location of sampling. Furthermore, a shared pool of generalist bacterial taxa was detected independently of sampling location, plant species, or plant compartment. We conclude that the rhizosphere and phyllosphere of native plants in stressed Mediterranean ecosystems consist of common bacterial assemblages contributing to the survival of the plant, while at the same time the discrete soil properties and environmental pressures of each habitat drive the development of a complementary bacterial community with a distinct structure for each plant and location. We suggest that this trade-off between generalist and specialist bacterial community is tailored to benefit the symbiosis with the plant.

scholarly journals Bacterial community structure upstream and downstream of cascade dams along the Lancang River in southwestern China

2020 ◽  
Vol 27 (34) ◽  
pp. 42933-42947
Author(s):  
Xia Luo ◽  
Xinyi Xiang ◽  
Guoyi Huang ◽  
Xiaorui Song ◽  
Peijia Wang ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Spatial Distance ◽  
Rrna Gene ◽  
Lancang River ◽  
Hydroelectric Dam ◽  
Cascade Dams

Abstract Extensive construction of dams by humans has caused alterations in flow regimes and concomitant alterations in river ecosystems. Even so, bacterioplankton diversity in large rivers influenced by cascade dams has been largely ignored. In this study, bacterial community diversity and profiles of seven cascade dams along the720 km of the Lancang River were studied using Illumina sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Spatiotemporal variations of bacterial communities in sediment and water of the Gongguoqiao hydroelectric dam and factors affecting these variations were also examined. Microbial diversity and richness in surface water increased slightly from upstream toward downstream along the river. A significant positive correlation between spatial distance and dissimilarities in bacterial community structure was confirmed (Mantel test, r = 0.4826, p = 0.001). At the Gongguoqiao hydroelectric dam, temporal differences in water overwhelmed spatial variability in bacterial communities. Temperature, precipitation, and nutrient levels were major drivers of seasonal microbial changes. Most functional groups associated with carbon cycling in sediment samples decreased from winter to summer. Our findings improve our understanding of associations, compositions, and predicted functional profiles of microbial communities in a large riverine ecosystem influenced by multiple cascade dams.

scholarly journals Impact of long-term industrial contamination on the bacterial communities in urban river sediments

2020 ◽  
Author(s):  
lei zhang ◽  
Demei Tu ◽  
Xingchen Li ◽  
Wenxuan Lu ◽  
Jing Li
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Industrial Pollution ◽  
River Sediments ◽  
Urban River ◽  
Bacterial Phyla ◽  
Different Types

Abstract Background: The contamination of the aquatic environment of urban rivers with industrial wastewater has affected the abiotic conditions and biological activities of the trophic levels of the ecosystem, particularly sediments. However, most current research about microorganism in urban aquatic environments has focused on indicator bacteria related to feces and organic pollution. Meanwhile, they ignored the interactions among microorganisms. To deeply understand the impact of industrial contamination on microbial community, we study the bacterial community structure and diversity in river sediments under the influence of different types of industrial pollution by Illumina MiSeq high-throughput sequencing technology and conduct a more detailed analysis of microbial community structure through co-occurrence networks.Results: Although the composition of dominant bacterial phyla in different sediment samples was similar, their relative abundance was different. These dominant bacterial phyla showed significant differences in different types of industrial contaminated sediment. In addition, redundancy analysis indicated that the structure of the bacterial community in river sediments was influenced by a variety of environmental factors. TN, TP, TOC and metals (Cu, Zn and Cd) were the most important driving factors that determined the bacterial community in urban river sediments (P <0.01). According to PICRUSt analysis, the bacterial communities in different locations had similar overall functional profiles. It is worth noting that the 15 functional genes related to xenobiotics biodegradation and metabolism were the most abundant in the same location. The non-random assembly patterns of bacterial composition in different types of industrially polluted sediments were determined by a co-occurrence network. Environmental conditions resulting from different industrial pollutants may play an important role in determining their co-occurrence patterns of these bacterial taxa. Among them, the bacterial taxa involved in carbon and nitrogen cycles in module I were relatively abundant, and the bacterial taxa in module II were involved in the repair of metal pollution.Conclusions: Our data indicate that long-term potential interactions between different types of industrial pollution and taxa collectively affect the structure of the bacterial community in urban river sediments.

scholarly journals Variation of rhizosphere bacterial community diversity in the desert ephemeral plant Ferula sinkiangensis across environmental gradients

2020 ◽  
Author(s):  
Zhang tao ◽  
Wang Zhongke ◽  
Lv Xinhua ◽  
Dang Hanli ◽  
Zhuang Li
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Community Diversity ◽  
Bacterial Community Diversity ◽  
Physicochemical Factors ◽  
Rhizosphere Bacterial Community ◽  
Ferula Sinkiangensis ◽  
Rhizosphere Bacterial Communities

Ferula sinkiangensis is a desert short-lived medicinal plant, and its number is rapidly decreasing. Rhizosphere microbial community plays an important role in plant growth and adaptability. However, Ferula sinkiangensis rhizosphere bacterial communities and the soil physicochemical factors that drive the bacterial community distribution are currently unclear. On this study, based on high-throughput sequencing, we explored the diversity, structure and composition of Ferula sinkiangensis rhizosphere bacterial communities at different slope positions and soil depths and their correlation with soil physicochemical properties. Our results revealed the heterogeneity and variation trends of Ferula sinkiangensis rhizosphere bacterial community diversity and abundance on a fine spatial scale (Slope position and soil depth) and Found Actinobacteria (25.5%), Acidobacteria (16.9%), Proteobacteria (16.6%), Gemmatimonadetes (11.5%) and Bacteroidetes (5.8%) were the dominant bacterial phyla in Ferula sinkiangensi s rhizosphere soil. Among all soil physicochemical variables shown in this study, there was a strong positive correlation between phosphorus (AP) and the diversity of rhizosphere bacterial community in Ferula sinkiangensis . In addition, Soil physicochemical factors jointly explained 24.28% of variation in Ferula sinkiangensis rhizosphere bacterial community structure. Among them, pH largely explained the variation of Ferula sinkiangensis rhizosphere bacterial community structure (5.58%), followed by total salt (TS, 5.21%) and phosphorus (TP, 4.90%).

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