scholarly journals High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

2007 ◽  
Vol 104 (35) ◽  
pp. 13936-13941 ◽  
Author(s):  
S. Newstead ◽  
H. Kim ◽  
G. von Heijne ◽  
S. Iwata ◽  
D. Drew
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Protein ◽  
High Throughput ◽  
Protein Overexpression ◽  
Eukaryotic Membrane Protein

scholarly journals Phenotypic effects of membrane protein overexpression in Saccharomyces cerevisiae

2006 ◽  
Vol 103 (30) ◽  
pp. 11148-11153 ◽  
Author(s):  
M. Osterberg ◽  
H. Kim ◽  
J. Warringer ◽  
K. Melen ◽  
A. Blomberg ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Membrane Protein ◽  
Protein Overexpression

High‐throughput Cell‐free Screening of Eukaryotic Membrane Protein Expression in Lipidic Mimetics

2021 ◽  
Author(s):  
Renato Bruni ◽  
Aisha Laguerre ◽  
Anna‐Maria Kaminska ◽  
Sean McSweeney ◽  
Wayne A. Hendrickson ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Expression ◽  
High Throughput ◽  
Membrane Protein Expression ◽  
Eukaryotic Membrane Protein

scholarly journals Comparative Roles of the Cell Wall and Cell Membrane in Limiting Uptake of Xenobiotic Molecules by Saccharomyces cerevisiae

2003 ◽  
Vol 47 (6) ◽  
pp. 2012-2014 ◽  
Author(s):  
Mustapha Aouida ◽  
Omar Tounekti ◽  
Omrane Belhadj ◽  
Lluis M. Mir
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Wall ◽  
Plasma Membrane ◽  
Cell Membrane ◽  
Membrane Protein

ABSTRACT Using reversible electropermeabilization of cells and spheroplasts, we show that the cell wall and plasma membrane partly account for bleomycin resistance by acting as two independent barriers. We also report on the presence of a membrane protein that may be responsible for bleomycin internalization and toxicity in Saccharomyces cerevisiae.

scholarly journals Improving recombinant eukaryotic membrane protein yields inPichia pastoris: The importance of codon optimization and clone selection

2011 ◽  
Vol 28 (6) ◽  
pp. 398-411 ◽  
Author(s):  
Fredrik Öberg ◽  
Jennie Sjöhamn ◽  
Matthew T. Conner ◽  
Roslyn M. Bill ◽  
Kristina Hedfalk
Keyword(s):  
Membrane Protein ◽  
Codon Optimization ◽  
Clone Selection ◽  
Eukaryotic Membrane Protein

Towards improved membrane protein production in Pichia pastoris: General and specific transcriptional response to membrane protein overexpression

2014 ◽  
Vol 31 (6) ◽  
pp. 538-552 ◽  
Author(s):  
Thomas Vogl ◽  
Gerhard G. Thallinger ◽  
Guenther Zellnig ◽  
David Drew ◽  
James M. Cregg ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Membrane Protein ◽  
Protein Production ◽  
Transcriptional Response ◽  
Protein Overexpression
Sign in / Sign up

Export Citation Format

Share Document