scholarly journals Atomic structure of the 75 MDa extremophile Sulfolobus turreted icosahedral virus determined by CryoEM and X-ray crystallography

2013 ◽  
Vol 110 (14) ◽  
pp. 5504-5509 ◽  
Author(s):  
D. Veesler ◽  
T.-S. Ng ◽  
A. K. Sendamarai ◽  
B. J. Eilers ◽  
C. M. Lawrence ◽  
...  
Virology ◽  
1994 ◽  
Vol 204 (2) ◽  
pp. 777-788 ◽  
Author(s):  
Claudine Porta ◽  
Guoji Wang ◽  
Holland Cheng ◽  
Zhongguo Chen ◽  
Timothy S. Baker ◽  
...  

2013 ◽  
Vol 19 (S2) ◽  
pp. 52-53
Author(s):  
D. Veesler ◽  
T.-S. Ng ◽  
A.K. Sendamarai ◽  
B.J. Eilers ◽  
C.M. Lawrence ◽  
...  

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.


Nature ◽  
1992 ◽  
Vol 355 (6357) ◽  
pp. 275-278 ◽  
Author(s):  
Guoji Wang ◽  
Claudine Porta ◽  
Zhongguo Chen ◽  
Timothy S. Baker ◽  
John E. Johnson

1977 ◽  
Vol 41 (317) ◽  
pp. 7-26 ◽  
Author(s):  
Kingsley Dunham

SummaryThe Mineralogical Society celebrated its 50th Anniversary in 1926; this address attempts to summarize the substantial developments in the subject over the past 50 years and looks ahead to the future. Up to 1926, only the techniques of goniometry, optical investigation of non-opaques, and classical chemical analysis had been fully exploited. X-ray crystallography, though already 14 years old, had made little impact but was to advance with great rapidity to achieve, for example, the modern conception of silicate structure by 1934. The insight into the atomic structure of minerals provided by this technique has been central to the advancement of the subject. The powder camera and more recently the counting diffractometer have become the chief determinative tools, though microscopy is still important. Diffractometry with a tied computer has greatly speeded up structural work. Advances in electron microscopy may soon make it possible to produce direct images of atomic structure. Meanwhile wet-chemical procedures have largely given place to spectroscopy (optical emission, X-ray, and atomic absorption), to the electron microprobe and to various still more advanced techniques, including the investigation of stable and unstable isotopic composition by mass spectrometry. Independently, the optical identification of opaque minerals has been brought to an advanced state. All these developments have opened the way for progress in the understanding of the paragenesis and genesis of minerals, subjects to which experimentation at high temperatures and pressure now contribute materially; and for practical applications in many fields, including ore deposits, beneficiation, ceramics, refractories, cement, sinters, and fuels.


Author(s):  
Jules S. Jaffe ◽  
Robert M. Glaeser

Although difference Fourier techniques are standard in X-ray crystallography it has only been very recently that electron crystallographers have been able to take advantage of this method. We have combined a high resolution data set for frozen glucose embedded Purple Membrane (PM) with a data set collected from PM prepared in the frozen hydrated state in order to visualize any differences in structure due to the different methods of preparation. The increased contrast between protein-ice versus protein-glucose may prove to be an advantage of the frozen hydrated technique for visualizing those parts of bacteriorhodopsin that are embedded in glucose. In addition, surface groups of the protein may be disordered in glucose and ordered in the frozen state. The sensitivity of the difference Fourier technique to small changes in structure provides an ideal method for testing this hypothesis.


Author(s):  
S. Cusack ◽  
J.-C. Jésior

Three-dimensional reconstruction techniques using electron microscopy have been principally developed for application to 2-D arrays (i.e. monolayers) of biological molecules and symmetrical single particles (e.g. helical viruses). However many biological molecules that crystallise form multilayered microcrystals which are unsuitable for study by either the standard methods of 3-D reconstruction or, because of their size, by X-ray crystallography. The grid sectioning technique enables a number of different projections of such microcrystals to be obtained in well defined directions (e.g. parallel to crystal axes) and poses the problem of how best these projections can be used to reconstruct the packing and shape of the molecules forming the microcrystal.Given sufficient projections there may be enough information to do a crystallographic reconstruction in Fourier space. We however have considered the situation where only a limited number of projections are available, as for example in the case of catalase platelets where three orthogonal and two diagonal projections have been obtained (Fig. 1).


Author(s):  
Robert A. Grant ◽  
Laura L. Degn ◽  
Wah Chiu ◽  
John Robinson

Proteolytic digestion of the immunoglobulin IgG with papain cleaves the molecule into an antigen binding fragment, Fab, and a compliment binding fragment, Fc. Structures of intact immunoglobulin, Fab and Fc from various sources have been solved by X-ray crystallography. Rabbit Fc can be crystallized as thin platelets suitable for high resolution electron microscopy. The structure of rabbit Fc can be expected to be similar to the known structure of human Fc, making it an ideal specimen for comparing the X-ray and electron crystallographic techniques and for the application of the molecular replacement technique to electron crystallography. Thin protein crystals embedded in ice diffract to high resolution. A low resolution image of a frozen, hydrated crystal can be expected to have a better contrast than a glucose embedded crystal due to the larger density difference between protein and ice compared to protein and glucose. For these reasons we are using an ice embedding technique to prepare the rabbit Fc crystals for molecular structure analysis by electron microscopy.


Author(s):  
Y. Y. Wang ◽  
H. Zhang ◽  
V. P. Dravid ◽  
H. Zhang ◽  
L. D. Marks ◽  
...  

Azuma et al. observed planar defects in a high pressure synthesized infinitelayer compound (i.e. ACuO2 (A=cation)), which exhibits superconductivity at ~110 K. It was proposed that the defects are cation deficient and that the superconductivity in this material is related to the planar defects. In this report, we present quantitative analysis of the planar defects utilizing nanometer probe xray microanalysis, high resolution electron microscopy, and image simulation to determine the chemical composition and atomic structure of the planar defects. We propose an atomic structure model for the planar defects.Infinite-layer samples with the nominal chemical formula, (Sr1-xCax)yCuO2 (x=0.3; y=0.9,1.0,1.1), were prepared using solid state synthesized low pressure forms of (Sr1-xCax)CuO2 with additions of CuO or (Sr1-xCax)2CuO3, followed by a high pressure treatment.Quantitative x-ray microanalysis, with a 1 nm probe, was performed using a cold field emission gun TEM (Hitachi HF-2000) equipped with an Oxford Pentafet thin-window x-ray detector. The probe was positioned on the planar defects, which has a 0.74 nm width, and x-ray emission spectra from the defects were compared with those obtained from vicinity regions.


Sign in / Sign up

Export Citation Format

Share Document