scholarly journals ON THE MECHANISM OF INHIBITION OF DEOXYRIBONUCLEIC ACID SYNTHESIS IN EHRLICH ASCITES TUMOR CELLS BY DEOXYADENOSINE IN VITRO

1961 ◽  
Vol 47 (5) ◽  
pp. 680-686 ◽  
Author(s):  
K. Overgaard-Hansen ◽  
H. Klenow
Keyword(s):  
Tumor Cells ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Mechanism Of Inhibition ◽  
Deoxyribonucleic Acid Synthesis ◽  
Ehrlich Ascites

Effects of Actinomycin D on Purine Ribonucleotide Metabolism in Ehrlich Ascites Tumor Cells In Vitro

1974 ◽  
Vol 52 (3) ◽  
pp. 263-267 ◽  
Author(s):  
Floyd F. Snyder ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Actinomycin D ◽  
De Novo ◽  
Acid Synthesis ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

Actinomycin D treatment of Ehrlich ascites tumor cells in vitro causes slight to moderate inhibition of purine ribonucleotide synthesis de novo and from purine bases, and strong inhibition of inosinate dehydrogenase activity. These effects have the same dose–response relationship as inhibition of RNA synthesis by this drug. Daunomycin has similar effects on purine metabolism at a concentration that substantially inhibits nucleic acid synthesis. Actinomycin D treatment leads to elevated intracellular concentrations of ATP and GTP, and the effects of this drug on purine metabolism are believed to be mediated by these purine ribonucleoside triphosphates.

Effects of Bikaverin on purine nucleotide synthesis and catabolism in ehrlich ascites tumor cells in vitro

1977 ◽  
Vol 26 (21) ◽  
pp. 1973-1977 ◽  
Author(s):  
J.Frank Henderson ◽  
Mary L. Battell ◽  
George Zombor ◽  
Jan Fuska ◽  
P. Nemec
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Purine Nucleotide ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Nucleotide Synthesis ◽  
Ehrlich Ascites

NUCLEIC ACTD METABOLISM IN INTESTINAL MUCOSA: IV. THE EFFECT OF GLUCOSE ON INCORPORATION OF C14-FORMATE INTO PURINES AND PYRIMIDINES IN VITRO

1963 ◽  
Vol 41 (1) ◽  
pp. 1557-1564
Author(s):  
Beryl E. Stewart ◽  
S. H. Zbarsky
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Rat Intestine ◽  
Maximal Stimulation ◽  
Ehrlich Ascites ◽  
Effect Of Glucose ◽  
Rna And Dna

Slices of rat intestine were incubated in Krebs–Ringer phosphate buffer in the presence of C14-formate. The addition of glucose to the buffer stimulated the incorporation of radioactivity into the purines and, in some instances, the pyrimidines of the acid-soluble fraction and nucleic acids of the tissue. With Ehrlich ascites tumor cells studied under similar conditions, the increase in uptake of C14-formate into the purines was somewhat greater. The data suggest also that maximal stimulation with slices of intestine is obtained at glucose concentrations somewhat higher than that required with Ehrlich ascites tumor cells. A finding of interest was the increased incorporation of C14-formate into the thymine of the intestinal DNA in the presence of glucose. This result has not been observed with Ehrlich ascites tumor cells. Slices of rat intestine incubated for 2 hours in the presence of glucose had a higher content of RNA and DNA than tissue not exposed to added glucose.

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