Effects of Bikaverin on purine nucleotide synthesis and catabolism in ehrlich ascites tumor cells in vitro

1977 ◽  
Vol 26 (21) ◽  
pp. 1973-1977 ◽  
Author(s):  
J.Frank Henderson ◽  
Mary L. Battell ◽  
George Zombor ◽  
Jan Fuska ◽  
P. Nemec
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Purine Nucleotide ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Nucleotide Synthesis ◽  
Ehrlich Ascites

NUCLEIC ACTD METABOLISM IN INTESTINAL MUCOSA: IV. THE EFFECT OF GLUCOSE ON INCORPORATION OF C14-FORMATE INTO PURINES AND PYRIMIDINES IN VITRO

1963 ◽  
Vol 41 (1) ◽  
pp. 1557-1564
Author(s):  
Beryl E. Stewart ◽  
S. H. Zbarsky
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Rat Intestine ◽  
Maximal Stimulation ◽  
Ehrlich Ascites ◽  
Effect Of Glucose ◽  
Rna And Dna

Slices of rat intestine were incubated in Krebs–Ringer phosphate buffer in the presence of C14-formate. The addition of glucose to the buffer stimulated the incorporation of radioactivity into the purines and, in some instances, the pyrimidines of the acid-soluble fraction and nucleic acids of the tissue. With Ehrlich ascites tumor cells studied under similar conditions, the increase in uptake of C14-formate into the purines was somewhat greater. The data suggest also that maximal stimulation with slices of intestine is obtained at glucose concentrations somewhat higher than that required with Ehrlich ascites tumor cells. A finding of interest was the increased incorporation of C14-formate into the thymine of the intestinal DNA in the presence of glucose. This result has not been observed with Ehrlich ascites tumor cells. Slices of rat intestine incubated for 2 hours in the presence of glucose had a higher content of RNA and DNA than tissue not exposed to added glucose.

Growth acceleration of Ehrlich ascites tumor cells treated by proteinase in vitro

1989 ◽  
Vol 25 (12) ◽  
pp. 1837-1841 ◽  
Author(s):  
Irenäus A. Adamietz ◽  
Fritz Kurfürst ◽  
Ulrich Müller ◽  
Karlheinz Renner ◽  
Manfred Rimpler
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

Effects of Actinomycin D on Purine Ribonucleotide Metabolism in Ehrlich Ascites Tumor Cells In Vitro

1974 ◽  
Vol 52 (3) ◽  
pp. 263-267 ◽  
Author(s):  
Floyd F. Snyder ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Actinomycin D ◽  
De Novo ◽  
Acid Synthesis ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

Actinomycin D treatment of Ehrlich ascites tumor cells in vitro causes slight to moderate inhibition of purine ribonucleotide synthesis de novo and from purine bases, and strong inhibition of inosinate dehydrogenase activity. These effects have the same dose–response relationship as inhibition of RNA synthesis by this drug. Daunomycin has similar effects on purine metabolism at a concentration that substantially inhibits nucleic acid synthesis. Actinomycin D treatment leads to elevated intracellular concentrations of ATP and GTP, and the effects of this drug on purine metabolism are believed to be mediated by these purine ribonucleoside triphosphates.

Relative importance of alternative pathways of purine nucleotide biosynthesis in Ehrlich ascites tumor cells in vivo

1976 ◽  
Vol 54 (4) ◽  
pp. 341-349 ◽  
Author(s):  
Camilla M. Smith ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Tumor ◽  
Relative Importance ◽  
Purine Nucleotide ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Alternative Pathways ◽  
Ehrlich Ascites ◽  
In Cells

Several alternative pathways of purine nucleotide synthesis coexist in cells and the relative importance of each pathway for maintaining purine nucleotide concentrations in cells has been studied. Specific inhibitors were used to block these synthetic routes in Ehrlich ascites tumor cells in vivo and the effect of inhibiting each pathway was evaluated by measuring intracellular purine nucleotide concentrations by high-speed liquid chromatography. The results of this study indicate that adenosine triphosphate and guanosine triphosphate concentrations of Ehrlich ascites tumor cells are maintained primarily by purine biosynthesis de novo although other pathways do make significant contributions.

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