Effects of Actinomycin D on Purine Ribonucleotide Metabolism in Ehrlich Ascites Tumor Cells In Vitro

1974 ◽  
Vol 52 (3) ◽  
pp. 263-267 ◽  
Author(s):  
Floyd F. Snyder ◽  
J. Frank Henderson
Keyword(s):  
Tumor Cells ◽  
Actinomycin D ◽  
De Novo ◽  
Acid Synthesis ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

Actinomycin D treatment of Ehrlich ascites tumor cells in vitro causes slight to moderate inhibition of purine ribonucleotide synthesis de novo and from purine bases, and strong inhibition of inosinate dehydrogenase activity. These effects have the same dose–response relationship as inhibition of RNA synthesis by this drug. Daunomycin has similar effects on purine metabolism at a concentration that substantially inhibits nucleic acid synthesis. Actinomycin D treatment leads to elevated intracellular concentrations of ATP and GTP, and the effects of this drug on purine metabolism are believed to be mediated by these purine ribonucleoside triphosphates.

Effects of misonidazole on purine metabolism in ehrlich ascites tumor cells in vitro

1980 ◽  
Vol 29 (18) ◽  
pp. 2533-2536 ◽  
Author(s):  
J.Frank Henderson ◽  
George Zombor
Keyword(s):  
Tumor Cells ◽  
Purine Metabolism ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites

The Degradation of Newly Synthesized RNA in Ehrlich Ascites Tumor Cells

1973 ◽  
Vol 51 (5) ◽  
pp. 495-505 ◽  
Author(s):  
Richard G. von Tigerstrom
Keyword(s):  
Tumor Cells ◽  
Rna Synthesis ◽  
Actinomycin D ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites ◽  
Ehrlich Ascites Cells ◽  
The Stability

The stability of rapidly labelled RNA in Ehrlich ascites tumor cells under in vitro conditions was investigated. [2-14C]Uridine was effectively incorporated into RNA and 90% of the acid-insoluble isotope was associated with the nucleus after 30 min labelling. When RNA synthesis was inhibited by actinomycin D or when [14C]uridine was chased with [12C]uridine at this time, a rapid degradation of approximately 50% of the labelled RNA could be observed. The rates of degradation, the extent of degradation under certain incubation conditions, and the classes of RNA from which the isotope was released were almost identical when the two chase techniques were compared. The concentration of uridine used during the chase did not inhibit RNA synthesis significantly as judged from incorporation of [8-14C]guanine. The results indicated that the degradation of the newly synthesized RNA in Ehrlich ascites cells occurred naturally and was not induced by actinomycin D.

Extramitochondrial Fatty Acid Synthesis in Ehrlich Ascites Tumor Cells Propagated in Vitro and in Vivo

1972 ◽  
Vol 141 (2) ◽  
pp. 506-509 ◽  
Author(s):  
B. N. Pedersen ◽  
A. Gromek ◽  
J. L. Daehnfeldt
Keyword(s):  
Fatty Acid ◽  
Tumor Cells ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Ehrlich Ascites Tumor ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Ehrlich Ascites
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