A monoclonal antibody-enzyme immunoassay for serum carcinoembryonic antigen with increased specificity for carcinomas.

An enzyme immunoassay kit for carcinoembryonic antigen (CEA) that uses plastic beads coated with guinea-pig anti-CEA as first antibody and goat anti-CEA conjugated with horseradish peroxidase as second antibody has been evaluated. The method does not involve perchloric acid extraction and therefore avoids a dialysis procedure. It is accurate, sensitive, and inexpensive to operate, and provides values comparable to those obtained with the Roche-CEA Z-Gel radioimmunoassay.

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Human anti-murine antibody interference in measurement of carcinoembryonic antigen assessed with a double-antibody enzyme immunoassay

Clinical Chemistry ◽

10.1093/clinchem/37.1.51 ◽

1991 ◽

Vol 37 (1) ◽

pp. 51-57 ◽

Cited By ~ 18

Author(s):

Theresa Price ◽

Barbara G Beatty ◽

J David Beatty ◽

Alan J McNally

Keyword(s):

Monoclonal Antibody ◽

Plasma Concentration ◽

Carcinoembryonic Antigen ◽

Enzyme Immunoassay ◽

Surgical Resection ◽

Clinical Evidence ◽

Murine Mabs ◽

Murine Antibody

Abstract Fifty-eight plasma specimens from 30 patients who had undergone presurgical radioimmunoscintigraphy with 111In-labeled anti-carcinoembryonic antigen (CEA) murine monoclonal antibody (Mab) and who had no clinical evidence of disease after surgical resection showed increased concentrations of CEA (greater than or equal to 5 micrograms/L) in plasma when studied with the previously available commercial CEA enzyme immunoassay (EIA) from Roche. The possible role of anti-murine antibody (HAMA) interference was addressed by adding mouse IgG (mIgG) to the plasma (2 g/L) before assay. Fifteen specimens (26%) showed no change in CEA (reflecting a true increase as shown by the original results), 22 (38%) showed a decrease in CEA of greater than 15% but remained positive (reflecting an artefactual increase), and 21 (36%) became CEA-negative (less than or equal to 5 micrograms/L; reflecting a false increase). Subsequently, we assayed the same samples with a modified version of this CEA EIA kit and 47 specimens remained CEA positive (greater than 5 micrograms/L): 25 (53%) were truly increased, 12 (26%) remained artefactually increased, and 10 (21%) continued to show a false increase. The degree of interference in the original EIA kit correlated with the plasma concentration of HAMA (P less than 0.005). All artefactually and falsely increased CEA values observed in both kits were corrected by addition of polyclonal mIgG or of a mixture of IgG1, IgG2a, and IgG2b Mabs before assay. This correction is important in the follow-up of patients who receive murine Mabs for treatment or diagnosis.

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Monoclonal antibody imaging in patients with colorectal cancer and increasing levels of serum carcinoembryonic antigen. Experience with ZCE-025 and IMMU-4 monoclonal antibodies and proposed directions for clinical trials

Cancer ◽

10.1002/1097-0142(19930615)71:12+<4293::aid-cncr2820711818>3.0.co;2-r ◽

1993 ◽

Vol 71 (S12) ◽

pp. 4293-4297 ◽

Cited By ~ 10

Author(s):

Yehuda Z. Patt ◽

Donald A. Podoloff ◽

Stephen Curley ◽

Ruth Smith ◽

Vijaya A. Badkhamkar ◽

...

Keyword(s):

Colorectal Cancer ◽

Monoclonal Antibody ◽

Clinical Trials ◽

Monoclonal Antibodies ◽

Carcinoembryonic Antigen ◽

Antibody Imaging ◽

Serum Carcinoembryonic Antigen ◽

Monoclonal Antibody Imaging

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An enhanced chemiluminescent enzyme immunoassay for serum carcinoembryonic antigen based on a modification of a commercial kit

Journal of Bioluminescence and Chemiluminescence ◽

10.1002/bio.1170010106 ◽

1986 ◽

Vol 1 (1) ◽

pp. 29-34 ◽

Cited By ~ 7

Author(s):

R. Haggart ◽

G. H. G. Thorpe ◽

S. B. Moseley ◽

L. J. Kricka ◽

T. P. Whitehead

Keyword(s):

Carcinoembryonic Antigen ◽

Enzyme Immunoassay ◽

Serum Carcinoembryonic Antigen ◽

Commercial Kit ◽

Chemiluminescent Enzyme Immunoassay

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Technetium 99m-labeled IMMU-4, a monoclonal antibody against carcinoembryonic antigen, for imaging of occult recurrent colorectal cancer in patients with rising serum carcinoembryonic antigen levels.

Journal of Clinical Oncology ◽

10.1200/jco.1994.12.3.489 ◽

1994 ◽

Vol 12 (3) ◽

pp. 489-495 ◽

Cited By ~ 16

Author(s):

Y Z Patt ◽

D A Podoloff ◽

S Curley ◽

L Kasi ◽

R Smith ◽

...

Keyword(s):

Colorectal Cancer ◽

Monoclonal Antibody ◽

Carcinoembryonic Antigen ◽

Metastatic Cancer ◽

False Negative ◽

Exploratory Laparotomy ◽

Colorectal Disease ◽

Technetium 99M ◽

Serum Carcinoembryonic Antigen ◽

Sensitivity Specificity

PURPOSE We tested whether nuclear imaging with technetium 99m-labeled murine monoclonal antibody (MoAb) against carcinoembryonic antigen (CEA) IMMU-4 will detect recurrent colorectal disease in patients with a rising serum CEA level but negative abdominal and pelvic computed tomographic (CT) scan, chest radiograph, and colonoscopy, or barium enema. PATIENTS AND METHODS Sixteen patients with completely resected, CEA-producing colorectal cancer were given 1 mg of 99mTc-labeled IMMU-4 intravenously with no toxic side effects. Planar and single-photon emission CT (SPECT) scans were acquired at 6 hours. Fifteen patients underwent an exploratory laparotomy at 24 hours. Results of the scintigraphy were correlated with surgical findings. RESULTS Twelve of 15 patients (80%) had true-positive (TP) scans when correlated with surgery. Two of 15 (13%) had true-negative (TN) scans inasmuch as exploratory laparotomy failed to detect recurrent disease. A false-positive (FP) scan was obtained in one of 15 (7%). There were no false-negative (FN) scans. Sensitivity, specificity, accuracy, and the positive predictive value (PPV) were 100%, 67%, 93%, and 92%, respectively. Twenty-six histologically confirmed areas of malignancy were found and correlated with areas of increased activity seen on IMMU-4 scintigraphy. Twenty-one were TP; five were not detected by scintigraphy and were thus considered to be FN. There were five FP lesions and 25 TN regions. Sensitivity, specificity, accuracy, and the PPV in these 26 cancer tissues were 81%, 83%, 82%, and 81%, respectively. The median radioactivity ratio of tumorous tissue to normal tissue was 3.33, with a range of 0.89 to 17.16. CONCLUSIONS These results suggest that 99mTc IMMU-4 scintigraphy is an important addition to the armamentarium available for diagnostic imaging and may help detect occult metastatic cancer missed by abdominal and pelvic CT in patients with rising CEA levels.

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