Evaluation of a Commercial Enzyme Immunoassay Kit for Serum Carcinoembryonic Antigen

1981 ◽  
Vol 18 (4) ◽  
pp. 248-251 ◽  
Author(s):  
Dickran Fabricatorian ◽  
Neil D Gallagher
Keyword(s):  
Horseradish Peroxidase ◽  
Guinea Pig ◽  
Carcinoembryonic Antigen ◽  
Enzyme Immunoassay ◽  
Perchloric Acid ◽  
Acid Extraction ◽  
Commercial Enzyme ◽  
Serum Carcinoembryonic Antigen ◽  
Commercial Enzyme Immunoassay

An enzyme immunoassay kit for carcinoembryonic antigen (CEA) that uses plastic beads coated with guinea-pig anti-CEA as first antibody and goat anti-CEA conjugated with horseradish peroxidase as second antibody has been evaluated. The method does not involve perchloric acid extraction and therefore avoids a dialysis procedure. It is accurate, sensitive, and inexpensive to operate, and provides values comparable to those obtained with the Roche-CEA Z-Gel radioimmunoassay.

Extraction of human plasma or sera by heat treatment for a solid-phase radioimmunoassay of carcinoembryonic antigen.

1979 ◽  
Vol 25 (5) ◽  
pp. 773-776 ◽  
Author(s):  
Y D Kim ◽  
J T Tomita ◽  
J R Schenck ◽  
C Moeller ◽  
G F Weber ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Carcinoembryonic Antigen ◽  
Perchloric Acid ◽  
Plasma Proteins ◽  
Solid Phase ◽  
Precipitation Method ◽  
Acid Extraction ◽  
Serum Samples ◽  
Solid Phase Radioimmunoassay ◽  
Method Of Extraction

Abstract Heat treatment and a solid-phase radioimmunoassay are combined to give a relatively simple and rapid procedure for assay of carcinoembryonic antigen in plasma or serum. The new way we describe to extract this antigen is an alternative to the conventional method of extraction with perchloric acid. Heating plasma or serum samples in acetate buffer (0.16 mol/L, pH 5.0) at 70 degrees C for 15 min precipitates out most of the heat-labile, nonspecific plasma proteins, but leaves most of the antigen in solution, with its immunochemical properties apparently unaffected. Comparison between the heat treatment and the perchloric acid extraction yielded comparable values when tested either by solid-phase radioimmunoassay or by the zirconyl phosphate precipitation method. An added advantage of our method is that it gives the same assay values for both plasma and serum. Results for a group of pathological plasma samples, assayed by both our method and the perchloric acid-zirconyl phosphate precipitation method, gave a correlation coefficient of 0.90.

Carcinoembryonic Antigen and Cancer: A Comparative Study

1978 ◽  
Vol 64 (2) ◽  
pp. 151-160
Author(s):  
Luigi S. Azzolina ◽  
Girolamo Fracastoro ◽  
Nicola Pisarri ◽  
Renato Lievore ◽  
Claudio Cordiano ◽  
...  
Keyword(s):  
Comparative Study ◽  
Carcinoembryonic Antigen ◽  
Perchloric Acid ◽  
Clinical Usefulness ◽  
Precipitation Method ◽  
Acid Extraction ◽  
Malignant Diseases ◽  
Gastrointestinal Cancers ◽  
Antibody Method ◽  
Positive Results

The sera of 212 patients with malignant and non-malignant diseases have been radio-immunoassayed for the presence of the carcinoembryonic antigen (CEA) using 3 different kits produced by Hoffman La Roche, Switzerland (RCK), by Sorin-IRE, Italy and Belgium (SCK), and by the Istituto Sieroterapico Milanese, Italy (ICK). In the presence of endodermically-derived system carcinomas, the RCK gave more positive results (72.6%) than did the SCK (63.1%) or ICK (56.2%). With regard to other carcinomas, ICK (50.0%) and SCK (47.1%) gave better results than did RCK (30.6%). The results are discussed in terms of clinical usefulness of the CEA assay and as regards reproducibility, procedural advantages, and economical cost of each kit. It is concluded that the CEA assay cannot be used for the diagnosis of gastrointestinal cancers, although it is useful as a measure of « cancerosity » for prognostic purposes. In this sense the double antibody method employed by SCK and ICK is clinically more advantageous than is the perchloric acid extraction-zirconyl phosphate gel precipitation method of RCK.

Evaluation of a commercial enzyme immunoassay for HIV screening in urine

2004 ◽  
Vol 23 (11) ◽  
pp. 831-835 ◽  
Author(s):  
J. Almeda ◽  
J. Casabona ◽  
L. Matas ◽  
V. Gonz�lez ◽  
R. Muga ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Hiv Screening ◽  
Commercial Enzyme ◽  
Commercial Enzyme Immunoassay

scholarly journals Analysis of Abused Drugs in Human Urine with Commercial Enzyme Immunoassay Reagents.

Eisei kagaku ◽  
1996 ◽  
Vol 42 (2) ◽  
pp. 159-166 ◽  
Author(s):  
MUNEHIRO KATAGI ◽  
HIROSHI NISHIOKA ◽  
KUNIO NAKAJIMA ◽  
HITOSHI TSUCHIHASHI
Keyword(s):  
Enzyme Immunoassay ◽  
Human Urine ◽  
Commercial Enzyme ◽  
Abused Drugs ◽  
Commercial Enzyme Immunoassay
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