scholarly journals Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cell.

1984 ◽  
Vol 81 (13) ◽  
pp. 3978-3982 ◽  
Author(s):  
J. R. White ◽  
T. Ishizaka ◽  
K. Ishizaka ◽  
R. Sha'afi
Keyword(s):  
Mast Cell ◽  
Intracellular Concentration ◽  
Peritoneal Mast Cell ◽  
Free Calcium ◽  
Direct Demonstration

scholarly journals Mechanism for Increase in Intracellular Concentration of Free Calcium in Fertilized Sea Urchin Egg

1974 ◽  
Vol 63 (3) ◽  
pp. 374-388 ◽  
Author(s):  
Masahisa Nakamura ◽  
Ikuo Yasumasu
Keyword(s):  
Dissociation Constant ◽  
Sea Urchin ◽  
Calcium Ion ◽  
Calcium Binding ◽  
Intracellular Concentration ◽  
Sodium Ion ◽  
Free Calcium ◽  
Binding Ability ◽  
Sea Urchin Egg ◽  
Fertilized Egg

Intracellular free calcium concentration in the sea urchin egg was calculated to increase from 0.1 mM in an unfertilized egg to 1 mM in a fertilized egg 10 min after fertilization, based on measurement of the dissociation constant between free calcium and sea urchin egg homogenate. The dissociation constant between free calcium (dialyzable calcium) and homogenate of sea urchin eggs was measured by means of dialysis equilibrium. The dissociation constant of the unfertilized egg was about 10–4 M and that of the fertilized egg was about 10–3 M in three species of sea urchin, Hemicentrotus pulcherrimus, Anthocidaris crassispina, and Pseudocentrotus depressus. An increase in the dissociation constant of the unfertilized egg homogenate was observed after the addition of calcium ion at a concentration above 0.3 mM, the dissociation constant becoming the same as that observed in the fertilized egg homogenate after the administration of CaCl2 at a concentration above 1 mM. Sodium ion also caused a decrease in the calcium-binding ability of the unfertilized egg homogenate. Therefore, penetration of calcium ion or sodium ion upon fertilization might induce an increase in the dissociation constant and then intracellular concentration of free calcium would increase at fertilization. Almost all calcium-binding ability of the egg homogenate was found in the microsomal fraction, and the substance which bound calcium was thought to be protein in nature, since trypsin could decrease the level of calcium-binding substance in the homogenate of the eggs.

scholarly journals Peritoneal Mast Cell Stabilization and Free radical Scavenging activity of Yucca gloriosa L

2015 ◽  
Vol 7 (4s) ◽  
pp. 470-479 ◽  
Author(s):  
Saurabh Gupta ◽  
Satish Kumar Muthureddy Nataraj ◽  
K. Rama Satyanarayana Raju ◽  
Shashank Mulukutla ◽  
Nilesh Ambore ◽  
...  
Keyword(s):  
Mast Cell ◽  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Peritoneal Mast Cell ◽  
Scavenging Activity ◽  
Mast Cell Stabilization ◽  
Cell Stabilization

SKIN HEPARIN LEVELS AND POSTHEPARIN PLASMA CLEARING FACTOR LIPASE LEVELS IN IRRADIATED ADRENALECTOMIZED AND CORTISONE-TREATED RATS

1963 ◽  
Vol 41 (1) ◽  
pp. 1901-1906
Author(s):  
Frank C. Monkhouse ◽  
Donald G. Baker
Keyword(s):  
Mast Cell ◽  
Plasma Levels ◽  
Peritoneal Mast Cell ◽  
Rat Tissues ◽  
Cell Counts ◽  
X Irradiation ◽  
Mast Cell Count ◽  
Significant Change ◽  
Total Body ◽  
Adrenalectomized Rats

Experiments were carried out to determine whether changes in endogenous heparin in rat tissues were related to changes in plasma levels of clearing factor lipase (CFL). Skin heparin levels, postheparin plasma CFL levels, and peritoneal mast cell counts were measured in normal, adrenalectomized, cortisone-treated, and X-irradiated rats. Total body X-irradiation in doses of 400 r to 600 r decreased skin heparin levels. The postheparin CFL levels of irradiated rats remained high while that of pair-fed controls dropped significantly. Cortisone treatment for 6 days caused an increase in plasma CFL levels in both intact and adrenalectomized rats but no significant change in skin heparin levels or mast cell count. Adrenalectomy caused no significant change in either postheparin CFL or skin heparin levels.

scholarly journals Peritoneal mast cell stabilization potential of Pothos scandens L.

2013 ◽  
Vol 45 (1) ◽  
pp. 83 ◽  
Author(s):  
Saurabh Gupta ◽  
B Duraiswamy ◽  
MN Satishkumar
Keyword(s):  
Mast Cell ◽  
Peritoneal Mast Cell ◽  
Mast Cell Stabilization ◽  
Cell Stabilization

Dural mast cell degranulation is a putative mechanism for headache induced by PACAP-38

Cephalalgia ◽  
2012 ◽  
Vol 32 (4) ◽  
pp. 337-345 ◽  
Author(s):  
Michael Baun ◽  
Martin Holst Friborg Pedersen ◽  
Jes Olesen ◽  
Inger Jansen-Olesen
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Adenylate Cyclase ◽  
Phospholipase C ◽  
Dura Mater ◽  
Mast Cell Degranulation ◽  
Peritoneal Mast Cell ◽  
Selective Blockade ◽  
Peritoneal Mast Cells

Background: Pituitary adenylate cyclase activating peptide-38 (PACAP-38) has been shown to induce migraine in migraineurs, whereas the related peptide vasoactive intestinal peptide (VIP) does not. In the present study we examine the hypothesis that PACAP-38 and its truncated version PACAP-27 but not VIP cause degranulation of mast cells in peritoneum and in dura mater. Methods: The degranulatory effects of PACAP-38, PACAP-27 and VIP were investigated by measuring the amount of N-acetyl-β-hexosaminidase released from isolated peritoneal mast cells and from dura mater attached to the skull of the rat in vitro. In peritoneal mast cells N-truncated fragments of PACAP-38 (PACAP(6–38), PACAP(16–38) and PACAP(28–38)) were also studied. To investigate transduction pathways involved in mast cell degranulation induced by PACAP-38, PACAP-27 and VIP, the phospholipase C inhibitor U-73122 and the adenylate cyclase inhibitor SQ 22536 were used. Results: The peptides induced degranulation of isolated peritoneal mast cells of the rat with the following order of potency: PACAP-38 = PACAP(6–38) = PACAP(16–38) » PACAP-27 = VIP = PACAP(28–38). In the dura mater we found that 10−5 M PACAP-38 was significantly more potent in inducing mast cell degranulation than the same concentration of PACAP-27 or VIP. Inhibition of intracellular mechanisms demonstrated that PACAP-38-induced degranulation is mediated by the phospholipase C pathway. Selective blockade of the PAC1 receptor did not attenuate degranulation. Conclusion: These findings correlate with clinical studies and support the hypothesis that mast cell degranulation is involved in PACAP-induced migraine. PACAP-38 has a much stronger degranulatory effect on rat peritoneal and dural mast cells than VIP and PACAP-27. The difference in potency between PACAP-38- and PACAP-27/VIP-induced peritoneal mast cell degranulation is probably not related to the PAC1 receptor but is caused by a difference in efficacy on phospholipase C.

Nedocromil sodium modulates the function of long-term rat peritoneal mast cell cultures

1992 ◽  
Vol 26 (3) ◽  
pp. 293-303 ◽  
Author(s):  
Meir Shalit ◽  
Naomi Riesel-Yaron ◽  
Francesca Levi-Schaffer
Keyword(s):  
Mast Cell ◽  
Cell Cultures ◽  
Nedocromil Sodium ◽  
Peritoneal Mast Cell
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