scholarly journals Molecular cloning and characterization of the insecticidal crystal protein gene of Bacillus thuringiensis var. tenebrionis

1987 ◽  
Vol 84 (20) ◽  
pp. 7036-7040 ◽  
Author(s):  
V. Sekar ◽  
D. V. Thompson ◽  
M. J. Maroney ◽  
R. G. Bookland ◽  
M. J. Adang
Keyword(s):  
Bacillus Thuringiensis ◽  
Molecular Cloning ◽  
Protein Gene ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein

Expression of a Bacillus thuringiensis crystal protein gene in Pseudomonas cepacia 526

1990 ◽  
Vol 36 (12) ◽  
pp. 879-884 ◽  
Author(s):  
Carolyn A. Stock ◽  
Thomas J. McLoughlin ◽  
Janet A. Klein ◽  
Michael J. Adang
Keyword(s):  
Bacillus Thuringiensis ◽  
Manduca Sexta ◽  
Protein Gene ◽  
Crystal Protein ◽  
Pseudomonas Cepacia ◽  
Broad Host Range ◽  
Cry Protein ◽  
Insecticidal Crystal Protein ◽  
Tobacco Plants ◽  
Range Vector

An insecticidal crystal protein gene, cryIA(C), from Bacillus thuringiensis HD-1 was cloned into a broad host range vector, pSUP204, and the resulting plasmid, pSUP89A, was conjugated into a plant-colonizing bacterium, Pseudomonas cepacia 526. Southern blot analysis detected the presence of the cry gene in Pseudomonas cepacia 526 transconjugants. Production of a truncated insecticidal crystal protein in Pseudomonas cepacia was detected by Western blotting and ELISA techniques and was found to have insecticidal activity against the tobacco hornworm, Manduca sexta, in an artificial diet assay. We also followed the establishment of the Pseudomonas cepacia 526/Bt transconjugant on axenically grown tobacco plants and found that approximately 1% of the Pseudomonas cells retained pSUP89A 3 days after application. In spite of this instability, treated tobacco plants were protected from Manduca sexta infestation. Key words: Bacillus thuringiensis, Pseudomonas cepacia, insecticidal crystal protein, Cry protein.

Characterization and expression of a novel holotype insecticidal crystal protein gene from nativeBacillus thuringiensisBM59-2

2010 ◽  
Vol 56 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Aiping Zheng ◽  
Jun Zhu ◽  
Lingxia Wang ◽  
Shuangcheng Li ◽  
Qiming Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Protein Gene ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein ◽  
Escherichia Coli Bl21

We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740–3.499 µg/mL).

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