scholarly journals Isolation and characterization of a novel insecticidal crystal protein gene from Bacillus thuringiensis subsp. aizawai.

1991 ◽  
Vol 173 (13) ◽  
pp. 3966-3976 ◽  
Author(s):  
J A Chambers ◽  
A Jelen ◽  
M P Gilbert ◽  
C S Jany ◽  
T B Johnson ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Protein Gene ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein ◽  
Bacillus Thuringiensis Subsp ◽  
Isolation And Characterization

Expression and Characterization of a Recombinant Cry 1 Ac Crystal Protein Fused with Cry11A in Bacillus thuringiensis subsp. kurstaki Cry−B

2004 ◽  
Vol 7 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Jong Yul Roh ◽  
Ming Shun Li ◽  
Jin Hee Chang ◽  
Sang Chul Shin ◽  
Kyung Saeng Boo ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Crystal Protein ◽  
Bacillus Thuringiensis Subsp

Expression of a Bacillus thuringiensis crystal protein gene in Pseudomonas cepacia 526

1990 ◽  
Vol 36 (12) ◽  
pp. 879-884 ◽  
Author(s):  
Carolyn A. Stock ◽  
Thomas J. McLoughlin ◽  
Janet A. Klein ◽  
Michael J. Adang
Keyword(s):  
Bacillus Thuringiensis ◽  
Manduca Sexta ◽  
Protein Gene ◽  
Crystal Protein ◽  
Pseudomonas Cepacia ◽  
Broad Host Range ◽  
Cry Protein ◽  
Insecticidal Crystal Protein ◽  
Tobacco Plants ◽  
Range Vector

An insecticidal crystal protein gene, cryIA(C), from Bacillus thuringiensis HD-1 was cloned into a broad host range vector, pSUP204, and the resulting plasmid, pSUP89A, was conjugated into a plant-colonizing bacterium, Pseudomonas cepacia 526. Southern blot analysis detected the presence of the cry gene in Pseudomonas cepacia 526 transconjugants. Production of a truncated insecticidal crystal protein in Pseudomonas cepacia was detected by Western blotting and ELISA techniques and was found to have insecticidal activity against the tobacco hornworm, Manduca sexta, in an artificial diet assay. We also followed the establishment of the Pseudomonas cepacia 526/Bt transconjugant on axenically grown tobacco plants and found that approximately 1% of the Pseudomonas cells retained pSUP89A 3 days after application. In spite of this instability, treated tobacco plants were protected from Manduca sexta infestation. Key words: Bacillus thuringiensis, Pseudomonas cepacia, insecticidal crystal protein, Cry protein.

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