scholarly journals Production of avian leukosis virus particles in mammalian cells can be mediated by the interaction of the human immunodeficiency virus protein Rev and the Rev-responsive element.

1995 ◽  
Vol 92 (25) ◽  
pp. 11940-11944 ◽  
Author(s):  
G. Nasioulas ◽  
S. H. Hughes ◽  
B. K. Felber ◽  
J. M. Whitcomb
Keyword(s):  
Human Immunodeficiency Virus ◽  
Mammalian Cells ◽  
Avian Leukosis Virus ◽  
Virus Protein ◽  
Human Immunodeficiency Virus Protein ◽  
Virus Particles ◽  
Immunodeficiency Virus ◽  
Responsive Element

scholarly journals Mutation of the Rev-binding loop in the human immunodeficiency virus 1 leader causes a replication defect characterized by altered RNA trafficking and packaging

2006 ◽  
Vol 87 (10) ◽  
pp. 3039-3044 ◽  
Author(s):  
Jane S. Greatorex ◽  
Elizabeth A. Palmer ◽  
Roger J. Pomerantz ◽  
John A. Dangerfield ◽  
Andrew M. L. Lever
Keyword(s):  
Human Immunodeficiency Virus ◽  
Nuclear Export ◽  
Rna Packaging ◽  
Packaging Signal ◽  
Virus Particles ◽  
Immunodeficiency Virus ◽  
Responsive Element ◽  
Viral Genomic Rna ◽  
Human Immunodeficiency Virus 1

An internal RNA loop, located within the packaging signal of human immunodeficiency virus 1, that resembles the Rev-responsive element (RRE) closely was identified previously. Subsequent in vitro studies confirmed that the loop, termed loop A, could bind Rev protein specifically. Its proximity to the major splice donor has suggested a role for Rev–loop A interaction supplementary to or preceding that of the Rev–RRE interaction. To investigate this further in a replication-competent provirus, loop A was mutated to decrease its affinity for Rev. Impairing the Rev–loop A interaction led to reduced nuclear export of viral genomic RNA. RNA packaging decreased by approximately 30 %. Viral protein production and export of virus particles appeared normal; however, the virus was severely replication-deficient. The loop A sequence, which is 98 % conserved amongst viral isolates, is implicated in several cis-acting functions critical to virus viability.

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