scholarly journals RNA-binding protein TIAR is essential for primordial germ cell development

1998 ◽  
Vol 95 (5) ◽  
pp. 2331-2336 ◽  
Author(s):  
A. R. P. Beck ◽  
I. J. Miller ◽  
P. Anderson ◽  
M. Streuli
Keyword(s):  
Germ Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Primordial Germ Cell ◽  
Rna Binding Protein ◽  
Cell Development ◽  
Germ Cell Development

RNA binding protein Musashi‐1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus

2015 ◽  
Vol 29 (7) ◽  
pp. 2759-2768 ◽  
Author(s):  
Jessie M. Sutherland ◽  
Alexander P. Sobinoff ◽  
Barbara A. Fraser ◽  
Kate A. Redgrove ◽  
Tara‐Lynne Davidson ◽  
...  
Keyword(s):  
Germ Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Cell Development ◽  
Germ Cell Development

nos-1 and nos-2, two genes related to Drosophila nanos, regulate primordial germ cell development and survival in Caenorhabditis elegans

Development ◽  
1999 ◽  
Vol 126 (21) ◽  
pp. 4861-4871 ◽  
Author(s):  
K. Subramaniam ◽  
G. Seydoux
Keyword(s):  
Caenorhabditis Elegans ◽  
Germ Cell ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Primordial Germ Cell ◽  
Gene Families ◽  
Cell Development ◽  
Embryonic Patterning ◽  
Germ Cell Development ◽  
C Elegans

In Drosophila, the posterior determinant nanos is required for embryonic patterning and for primordial germ cell (PGC) development. We have identified three genes in Caenorhabditis elegans that contain a putative zinc-binding domain similar to the one found in nanos, and show that two of these genes function during PGC development. Like Drosophila nanos, C. elegans nos-1 and nos-2 are not generally required for PGC fate specification, but instead regulate specific aspects of PGC development. nos-2 is expressed in PGCs around the time of gastrulation from a maternal RNA associated with P granules, and is required for the efficient incorporation of PGCs into the somatic gonad. nos-1 is expressed in PGCs after gastrulation, and is required redundantly with nos-2 to prevent PGCs from dividing in starved animals and to maintain germ cell viability during larval development. In the absence of nos-1 and nos-2, germ cells cease proliferation at the end of the second larval stage, and die in a manner that is partially dependent on the apoptosis gene ced-4. Our results also indicate that putative RNA-binding proteins related to Drosophila Pumilio are required for the same PGC processes as nos-1 and nos-2. These studies demonstrate that evolutionarily distant organisms utilize conserved factors to regulate early germ cell development and survival, and that these factors include members of the nanos and pumilio gene families.

IGF-2 mRNA binding protein 2 regulates primordial germ cell development in zebrafish

2021 ◽  
pp. 113875
Author(s):  
Mingyu Li ◽  
Xiaozhi Rong ◽  
Ling Lu ◽  
Yun Li ◽  
Kai Yao ◽  
...  
Keyword(s):  
Germ Cell ◽  
Binding Protein ◽  
Primordial Germ Cell ◽  
Cell Development ◽  
Germ Cell Development ◽  
Mrna Binding Protein ◽  
Mrna Binding

scholarly journals Persistent Requirement and Alteration of the Key Targets of PRDM1 During Primordial Germ Cell Development in Mice1

2016 ◽  
Vol 94 (1) ◽  
Author(s):  
Chika Yamashiro ◽  
Takayuki Hirota ◽  
Kazuki Kurimoto ◽  
Tomonori Nakamura ◽  
Yukihiro Yabuta ◽  
...  
Keyword(s):  
Germ Cell ◽  
Primordial Germ Cell ◽  
Cell Development ◽  
Germ Cell Development

scholarly journals hnRNPH1 recruits PTBP2 and SRSF3 to cooperatively modulate alternative pre-mRNA splicing in germ cells and is essential for spermatogenesis and oogenesis

2021 ◽  
Author(s):  
Shuiqiao Yuan ◽  
Shenglei Feng ◽  
Jinmei Li ◽  
Hui Wen ◽  
Kuan Liu ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Germ Cell ◽  
Germ Cells ◽  
Rna Binding ◽  
Cell Development ◽  
Mrna Splicing ◽  
Conditional Knockout ◽  
Cell Junction ◽  
Germ Cell Development ◽  
Splicing Regulators

Abstract Coordinated regulation of alternative pre-mRNA splicing is essential for germ cell development. However, the molecular mechanism underlying that control alternative mRNA expression during germ cell development remains poorly understood. Herein, we showed that hnRNPH1, an RNA-binding protein, is highly expressed in the reproductive system and localized in the chromosomes of meiotic cells but excluded from the XY body in pachytene spermatocytes and recruits the splicing regulators PTBP2 and SRSF3 and cooperatively regulates the alternative splicing of the critical genes that are required for spermatogenesis. Conditional knockout Hnrnph1 in spermatogenic cells caused many abnormal splicing events that affect genes related to meiosis and communication between germ cells and Sertoli cells, characterized by asynapsis of chromosomes and impairment of germ-Sertoli communications, ultimately leading to male sterility. We further showed that hnRNPH1 could directly bind to SPO11 and recruit the splicing regulators PTBP2 and SRSF3 to regulate the alternative splicing of the target genes cooperatively. Strikingly, Hnrnph1 germline-specific mutant female mice were also infertile, and Hnrnph1-deficient oocytes exhibited a similar defective synapsis and cell-cell junction as shown in Hnrnph1-deficient male germ cells. Collectively, our data reveal an essential role for hnRNPH1 in regulating pre-mRNA splicing during spermatogenesis and oogenesis and support a molecular model whereby hnRNPH1 governs a network of alternative splicing events in germ cells via recruiting PTBP2 and SRSF3.

RNA-binding protein LIN28 is a sensitive marker of ovarian primitive germ cell tumours

2011 ◽  
Vol 59 (3) ◽  
pp. 452-459 ◽  
Author(s):  
Debin Xue ◽  
Yan Peng ◽  
Fenghua Wang ◽  
Robert W Allan ◽  
Dengfeng Cao
Keyword(s):  
Germ Cell ◽  
Rna Binding ◽  
Binding Protein ◽  
Rna Binding Protein ◽  
Germ Cell Tumours ◽  
Sensitive Marker

Dynamic changes in the subnuclear organisation of pre-mRNA splicing proteins and RBM during human germ cell development

1998 ◽  
Vol 111 (9) ◽  
pp. 1255-1265 ◽  
Author(s):  
D.J. Elliott ◽  
K. Oghene ◽  
G. Makarov ◽  
O. Makarova ◽  
T.B. Hargreave ◽  
...  
Keyword(s):  
Germ Cell ◽  
Cell Biology ◽  
Rna Binding ◽  
Spatial Association ◽  
Cell Types ◽  
Cell Development ◽  
Mrna Splicing ◽  
Human Testis ◽  
Germ Cell Development ◽  
Nuclear Regions

RBM is a germ-cell-specific RNA-binding protein encoded by the Y chromosome in all mammals, implying an important and evolutionarily conserved (but as yet unidentified) function during male germ cell development. In order to address this function, we have developed new antibody reagents to immunolocalise RBM in the different cell types in the human testis. We find that RBM has a different expression profile from its closest homologue hnRNPG. Despite its ubiquitous expression in all transcriptionally active germ cell types, RBM has a complex and dynamic cell biology in human germ cells. The ratio of RBM distributed between punctate nuclear structures and the remainder of the nucleoplasm is dynamically modulated over the course of germ cell development. Moreover, pre-mRNA splicing components are targeted to the same punctate nuclear regions as RBM during the early stages of germ cell development but late in meiosis this spatial association breaks down. After meiosis, pre-mRNA splicing components are differentially targeted to a specific region of the nucleus. While pre-mRNA splicing components undergo profound spatial reorganisations during spermatogenesis, neither heterogeneous ribonucleoproteins nor the transcription factor Sp1 show either developmental spatial reorganisations or any specific co-localisation with RBM. These results suggest dynamic and possibly multiple functions for RBM in germ cell development.

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