scholarly journals Requirement for an Interaction of XRCC4 with DNA Ligase IV for Wild-type V(D)J Recombination and DNA Double-strand Break Repairin Vivo

1998 ◽  
Vol 273 (38) ◽  
pp. 24708-24714 ◽  
Author(s):  
Ulf Grawunder ◽  
David Zimmer ◽  
Peter Kulesza ◽  
Michael R. Lieber
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Dna Ligase ◽  
Wild Type ◽  
Dna Ligase Iv ◽  
Dna Double Strand Break ◽  
Ligase Iv ◽  
Type V

Tetramerization and DNA Ligase IV Interaction of the DNA Double-strand Break Repair Protein XRCC4 are Mutually Exclusive

2003 ◽  
Vol 334 (2) ◽  
pp. 215-228 ◽  
Author(s):  
Mauro Modesti ◽  
Murray S. Junop ◽  
Rodolfo Ghirlando ◽  
Mandy van de Rakt ◽  
Martin Gellert ◽  
...  
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Ligase ◽  
Dna Ligase Iv ◽  
Dna Double Strand Break ◽  
Ligase Iv ◽  
Repair Protein ◽  
Break Repair

scholarly journals DNA Ligase IV Is Essential for V(D)J Recombination and DNA Double-Strand Break Repair in Human Precursor Lymphocytes

1998 ◽  
Vol 2 (4) ◽  
pp. 477-484 ◽  
Author(s):  
Ulf Grawunder ◽  
David Zimmer ◽  
Sebastian Fugmann ◽  
Klaus Schwarz ◽  
Michael R. Lieber
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Ligase ◽  
Dna Ligase Iv ◽  
Dna Double Strand Break ◽  
Ligase Iv ◽  
Break Repair

scholarly journals NAD+ is not utilized as a co-factor for DNA ligation by human DNA ligase IV

2020 ◽  
Vol 48 (22) ◽  
pp. 12746-12750
Author(s):  
Bailin Zhao ◽  
Tasmin Naila ◽  
Michael R Lieber ◽  
Alan E Tomkinson
Keyword(s):  
Strand Break ◽  
Dna Ligase ◽  
Unexpected Finding ◽  
Dna Ligases ◽  
Dna Ligase Iv ◽  
Human Dna ◽  
Dna Double Strand Break ◽  
Ligase Iv ◽  
Eukaryotic Dna ◽  
Catalytic Cycles

Abstract As nucleotidyl transferases, formation of a covalent enzyme-adenylate intermediate is a common first step of all DNA ligases. While it has been shown that eukaryotic DNA ligases utilize ATP as the adenylation donor, it was recently reported that human DNA ligase IV can also utilize NAD+ and, to a lesser extent ADP-ribose, as the source of the adenylate group and that NAD+, unlike ATP, enhances ligation by supporting multiple catalytic cycles. Since this unexpected finding has significant implications for our understanding of the mechanisms and regulation of DNA double strand break repair, we attempted to confirm that NAD+ and ADP-ribose can be used as co-factors by human DNA ligase IV. Here, we provide evidence that NAD+ does not enhance ligation by pre-adenylated DNA ligase IV, indicating that this co-factor is not utilized for re-adenylation and subsequent cycles of ligation. Moreover, we find that ligation by de-adenylated DNA ligase IV is dependent upon ATP not NAD+ or ADP-ribose. Thus, we conclude that human DNA ligase IV cannot use either NAD+ or ADP-ribose as adenylation donor for ligation.

scholarly journals Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF

2008 ◽  
Vol 36 (18) ◽  
pp. 5773-5786 ◽  
Author(s):  
Sumithra Jayaram ◽  
Gary Ketner ◽  
Noritaka Adachi ◽  
Les A. Hanakahi
Keyword(s):  
Strand Break ◽  
Double Strand Break ◽  
Double Strand Break Repair ◽  
Dna Ligase ◽  
Dna Ligase Iv ◽  
Ligase Iv ◽  
Break Repair
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