scholarly journals Immunoaffinity Purification and Functional Characterization of Human Transcription Factor IIH and RNA Polymerase II from Clonal Cell Lines That Conditionally Express Epitope-tagged Subunits of the Multiprotein Complexes

1998 ◽  
Vol 273 (51) ◽  
pp. 34444-34453 ◽  
Author(s):  
Eric Kershnar ◽  
Shwu-Yuan Wu ◽  
Cheng-Ming Chiang
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cell Lines ◽  
Functional Characterization ◽  
Clonal Cell ◽  
Immunoaffinity Purification ◽  
Clonal Cell Lines ◽  
Human Transcription Factor

ESTABLISHMENT AND CHARACTERIZATION OF CLONAL CELL LINES FROM THE VAGINA OF p53-DEFICIENT YOUNG MICE

2002 ◽  
Vol 38 (10) ◽  
pp. 547 ◽  
Author(s):  
KAYO TANAHASHI ◽  
SHINOBU SHIBAHARA ◽  
MINAKO OGAWA ◽  
MAKOTO HANAZONO ◽  
SHINICHI AIZAWA ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines ◽  
Young Mice

Establishment and Characterization of Clonal Cell Lines Derived from a Fibrosarcoma of the H2-K/v-jun Transgenic Mouse

Tumor Biology ◽  
2003 ◽  
Vol 24 (4) ◽  
pp. 176-184 ◽  
Author(s):  
Jiří Hatina ◽  
Lucie Hájková ◽  
Jan Peychl ◽  
Emil Rudolf ◽  
Jindřich Fínek ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines

scholarly journals A Region within the RAP74 Subunit of Human Transcription Factor IIF Is Critical for Initiation but Dispensable for Complex Assembly

1999 ◽  
Vol 19 (11) ◽  
pp. 7377-7387 ◽  
Author(s):  
Delin Ren ◽  
Lei Lei ◽  
Zachary F. Burton
Keyword(s):  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Residual Activity ◽  
Dna Supercoiling ◽  
Single Amino Acid ◽  
Phosphodiester Bond ◽  
Promoter Escape ◽  
Transcription Factor Iif ◽  
Human Transcription Factor

ABSTRACT Human transcription factor IIF (TFIIF) is an α2β2 heterotetramer of RNA polymerase II-associating 74 (RAP74) and RAP30 subunits. Mutagenic analysis shows that the N-terminal region of RAP74 between L155 (leucine at codon 155) and M177 is important for initiation. Mutants in this region have reduced activity in transcription, but none are inactive. Single amino acid substitutions at hydrophobic residues L155, W164, I176, and M177 have similar activity to RAP74(1–158), from which all but three amino acids of this region are deleted. Residual activity can be explained because each of these mutants forms a complex with RAP30 and recruits RNA polymerase II into the preinitiation complex. Mutants are defective for formation of the first phosphodiester bond from the adenovirus major late promoter but do not appear to have an additional significant defect in promoter escape. Negative DNA supercoiling partially compensates for the defects of TFIIF mutants in initiation, indicating that TFIIF may help to untwist the DNA helix for initiation.

Establishment and Characterization of Clonal Cell Lines from the Vagina of p53-Deficient Young Mice

2003 ◽  
Author(s):  
Kayo Tanahashi ◽  
Shinobu Shibahara ◽  
Minako Ogawa ◽  
Makoto Hanazono ◽  
Shinichi Aizawa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Clonal Cell ◽  
Clonal Cell Lines ◽  
Young Mice

scholarly journals Human Transcription Factor hTAFII150 (CIF150) Is Involved in Transcriptional Regulation of Cell Cycle Progression

1999 ◽  
Vol 19 (8) ◽  
pp. 5548-5556 ◽  
Author(s):  
Jay Martin ◽  
Robert Halenbeck ◽  
Jörg Kaufmann
Keyword(s):  
Cell Cycle ◽  
Transcription Factor ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cell Cycle Progression ◽  
Core Promoter ◽  
Cyclin B1 ◽  
Cycle Progression ◽  
Mammalian Cell Lines ◽  
Human Transcription Factor

ABSTRACT Here we present evidence that CIF150 (hTAFII150), the human homolog of Drosophila TAFII150, plays an important and selective role in establishing gene expression patterns necessary for progression through the cell cycle. Gel filtration experiments demonstrate that CIF150 (hTAFII150) seems to be less tightly associated with human transcription factor IID than hTAFII130 is associated with hTAFII250. The transient functional knockout of CIF150 (hTAFII150) protein led to cell cycle arrest at the G2/M transition in mammalian cell lines. PCR display analysis with the RNA derived from CIF150-depleted cells indicated that CIF150 (hTAFII150) is required for the transcription of only a subset of RNA polymerase II genes. CIF150 (hTAFII150) directly stimulated cyclin B1 and cyclin A transcription in cotransfection assays and in vitro assays, suggesting that the expression of these genes is dependent on CIF150 (hTAFII150) function. We defined a CIF150 (hTAFII150) consensus binding site and demonstrated that a CIF150-responsive cis element is present in the cyclin B1 core promoter. These results suggest that one function of CIF150 (hTAFII150) is to select specific RNA polymerase II core promoter elements involved in cell cycle progression.

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