scholarly journals Type XVII Collagen Regulates Lamellipod Stability, Cell Motility, and Signaling to Rac1 by Targeting Bullous Pemphigoid Antigen 1e to α6β4 Integrin

2011 ◽  
Vol 286 (30) ◽  
pp. 26768-26780 ◽  
Author(s):  
Kevin J. Hamill ◽  
Susan B. Hopkinson ◽  
Marcel F. Jonkman ◽  
Jonathan C. R. Jones
Keyword(s):  
Cell Motility ◽  
Bullous Pemphigoid ◽  
Bullous Pemphigoid Antigen ◽  
Α6β4 Integrin ◽  
Xvii Collagen

scholarly journals BPAG1e Maintains Keratinocyte Polarity through β4 Integrin–mediated Modulation of Rac 1 and Cofilin Activities

2009 ◽  
Vol 20 (12) ◽  
pp. 2954-2962 ◽  
Author(s):  
Kevin J. Hamill ◽  
Susan B. Hopkinson ◽  
Philip DeBiase ◽  
Jonathan C.R. Jones
Keyword(s):  
Signal Transduction ◽  
Cell Surface ◽  
Bullous Pemphigoid ◽  
Wild Type ◽  
Expression Levels ◽  
Bullous Pemphigoid Antigen ◽  
Affect Expression ◽  
Α6β4 Integrin ◽  
And Migration ◽  
Β4 Integrin

α6β4 integrin, a component of hemidesmosomes, also plays a role in keratinocyte migration via signaling through Rac1 to the actin-severing protein cofilin. Here, we tested the hypothesis that the β4 integrin-associated plakin protein, bullous pemphigoid antigen 1e (BPAG1e) functions as a scaffold for Rac1/cofilin signal transduction. We generated keratinocyte lines exhibiting a stable knockdown in BPAG1e expression. Knockdown of BPAG1e does not affect expression levels of other hemidesmosomal proteins, nor the amount of β4 integrin expressed at the cell surface. However, the amount of Rac1 associating with β4 integrin and the activity of both Rac1 and cofilin are significantly lower in BPAG1e-deficient cells compared with wild-type keratinocytes. In addition, keratinocytes deficient in BPAG1e exhibit loss of front-to-rear polarity and display aberrant motility. These defects are rescued by inducing expression of constitutively active Rac1 or active cofilin. These data indicate that the BPAG1e is required for efficient regulation of keratinocyte polarity and migration by determining the activation of Rac1.

scholarly journals The 97-kDa (LABD97) and 120-kDa (LAD-1) Fragments of Bullous Pemphigoid Antigen 180/Type XVII Collagen Have Different N-Termini

2003 ◽  
Vol 121 (6) ◽  
pp. 1554-1556 ◽  
Author(s):  
Yuji Nishizawa ◽  
Katrin Marcus ◽  
Helmut E. Meyer ◽  
Elke Butt ◽  
Katsushi Owaribe ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Bullous Pemphigoid Antigen ◽  
Xvii Collagen

scholarly journals IgG from Patients with Bullous Pemphigoid Depletes Cultured Keratinocytes of the 180-kDa Bullous Pemphigoid Antigen (Type XVII Collagen) and Weakens Cell Attachment

2009 ◽  
Vol 129 (4) ◽  
pp. 919-926 ◽  
Author(s):  
Hiroaki Iwata ◽  
Naoko Kamio ◽  
Yumi Aoyama ◽  
Yukari Yamamoto ◽  
Yoshiaki Hirako ◽  
...  
Keyword(s):  
Bullous Pemphigoid ◽  
Cell Attachment ◽  
Cultured Keratinocytes ◽  
Bullous Pemphigoid Antigen ◽  
Xvii Collagen
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