scholarly journals The Endocannabinoid, Anandamide, Augments Notch-1 Signaling in Cultured Cortical Neurons Exposed to Amyloid-β and in the Cortex of Aged Rats

2012 ◽  
Vol 287 (41) ◽  
pp. 34709-34721 ◽  
Author(s):  
Riffat Tanveer ◽  
Aoife Gowran ◽  
Janis Noonan ◽  
Sinead E. Keating ◽  
Andrew G. Bowie ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Amyloid Β ◽  
Aged Rats ◽  
Notch 1 ◽  
Cultured Cortical Neurons

scholarly journals The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons

Toxins ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 262
Author(s):  
Qin-Wei Wu ◽  
Josef P. Kapfhammer
Keyword(s):  
Rna Editing ◽  
Cortical Neurons ◽  
High Efficiency ◽  
Primary Cultures ◽  
Cultured Neurons ◽  
Therapeutic Tool ◽  
Bacterial Enzyme ◽  
Rna Targeting ◽  
Cultured Cortical Neurons ◽  
New Generation

The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is currently being developed as a new therapeutic tool for the treatment of neurological and other diseases. However, the safety of this new generation of RNA therapies is still unclear. In this study, we constructed a vector expressing CRISPR-Cas13 under a constitutive neuron-specific promoter. CRISPR-Cas13 from Leptotrichia wadei was expressed in primary cultures of mouse cortical neurons. We found that the presence of CRISPR-Cas13 impedes the development of cultured neurons. These results show a neurotoxic action of Cas13 and call for more studies to test for and possibly mitigate the toxic effects of Cas13 enzymes in order to improve CRISPR-Cas13-based tools for RNA targeting.

Protection of amyloid β protein (25–35)-induced neurotoxicity by methanol extract of Smilacis chinae rhizome in cultured rat cortical neurons

2006 ◽  
Vol 106 (2) ◽  
pp. 230-237 ◽  
Author(s):  
Ju Yeon Ban ◽  
Soon Ock Cho ◽  
Sang Bum Koh ◽  
Kyung-Sik Song ◽  
KiWhan Bae ◽  
...  
Keyword(s):  
Cortical Neurons ◽  
Methanol Extract ◽  
Amyloid Β ◽  
Amyloid Β Protein ◽  
Β Protein ◽  
Rat Cortical Neurons
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