scholarly journals Structure of the Type IVa Major Pilin from the Electrically Conductive Bacterial Nanowires ofGeobacter sulfurreducens

2013 ◽  
Vol 288 (41) ◽  
pp. 29260-29266 ◽  
Author(s):  
Patrick N. Reardon ◽  
Karl T. Mueller
Keyword(s):  
Electrically Conductive ◽  
Bacterial Nanowires

scholarly journals Structure of a cytochrome-based bacterial nanowire

2018 ◽  
Author(s):  
David J. Filman ◽  
Stephen F. Marino ◽  
Joy E. Ward ◽  
Lu Yang ◽  
Zoltán Mester ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Electron Transport ◽  
Long Range ◽  
Biological Significance ◽  
New Materials ◽  
Electrically Conductive ◽  
Cryo Electron Microscopy ◽  
Novel Structure ◽  
Bacterial Nanowires

AbstractElectrically conductive pili from Geobacter species, termed bacterial “nanowires”, are intensely studied for their biological significance and potential in the development of new materials. We have characterized a unique nanowire from conductive G. sulfurreducens pili preparations by cryo-electron microscopy composed solely of the c-type cytochrome OmcS. We present here, at 3.4 Å resolution, a novel structure of a cytochrome-based filament and discuss its possible role in long-range biological electron transport.Summary sentenceCryo-electron microscopy reveals the remarkable assembly of a c-type cytochrome into filaments comprising a heme-based bacterial nanowire.

scholarly journals Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms

2006 ◽  
Vol 103 (30) ◽  
pp. 11358-11363 ◽  
Author(s):  
Y. A. Gorby ◽  
S. Yanina ◽  
J. S. McLean ◽  
K. M. Rosso ◽  
D. Moyles ◽  
...  
Keyword(s):  
Shewanella Oneidensis ◽  
Electrically Conductive ◽  
Bacterial Nanowires

Electrically conductive bacterial nanowires in bisphosphonate-related osteonecrosis of the jaw biofilms

2013 ◽  
Vol 115 (1) ◽  
pp. 71-78 ◽  
Author(s):  
Greg Wanger ◽  
Yuri Gorby ◽  
Mohamed Y. El-Naggar ◽  
Thomas D. Yuzvinsky ◽  
Christoph Schaudinn ◽  
...  
Keyword(s):  
Osteonecrosis Of The Jaw ◽  
Electrically Conductive ◽  
Bacterial Nanowires

Cryogenic atomic force microscopy

1991 ◽  
Vol 49 ◽  
pp. 54-55
Author(s):  
K. A. Fisher ◽  
M. G. L. Gustafsson ◽  
M. B. Shattuck ◽  
J. Clarke
Keyword(s):  
Room Temperature ◽  
Rapid Freezing ◽  
Cryogenic Temperatures ◽  
Soft Or ◽  
Electrically Conductive ◽  
Force Microscopy ◽  
Flexible Molecules ◽  
Advantages And Disadvantages ◽  
Atomic Force ◽  
Chemical Perturbation

The atomic force microscope (AFM) is capable of imaging electrically conductive and non-conductive surfaces at atomic resolution. When used to image biological samples, however, lateral resolution is often limited to nanometer levels, due primarily to AFM tip/sample interactions. Several approaches to immobilize and stabilize soft or flexible molecules for AFM have been examined, notably, tethering coating, and freezing. Although each approach has its advantages and disadvantages, rapid freezing techniques have the special advantage of avoiding chemical perturbation, and minimizing physical disruption of the sample. Scanning with an AFM at cryogenic temperatures has the potential to image frozen biomolecules at high resolution. We have constructed a force microscope capable of operating immersed in liquid n-pentane and have tested its performance at room temperature with carbon and metal-coated samples, and at 143° K with uncoated ferritin and purple membrane (PM).

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